Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,855,562 | T→C | 51.3% | A56A (GCA→GCG) | yjeS ← | predicted Fe‑S electron transport protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,855,562 | 0 | T | C | 51.3% | 2.8 / 71.4 | 82 | A56A (GCA→GCG) | yjeS | predicted Fe‑S electron transport protein |
Reads supporting (aligned to +/- strand): ref base T (22/18); new base C (27/15); total (49/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTGATCACGCGCAGCGTACCGGGCAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCT > minE/2855500‑2855630 | cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:448005/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:1834429/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:3157092/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:320041/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:2956647/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:2913820/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:1981507/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:3323941/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:2875527/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:2868119/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:1524430/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:3356041/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:351204/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:2114643/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:212050/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:634352/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:1235635/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:1223239/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:814945/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:817203/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:272140/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:1105908/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:2191906/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:2289477/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:1029644/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGGCAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCa > 1:987121/1‑67 (MQ=255) cTGATCACGCGCAGCGTACCGGCCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:2425370/1‑67 (MQ=255) cTGATCACGCGCAGCGTACAGGGCAATAATTCGTGAGGACGAGCACGCAGCATACCGTGACGCGCCa > 1:1552137/1‑67 (MQ=255) cACGCGCAGCGTACCGGGCAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTc > 1:3022753/1‑69 (MQ=255) cACGCGCAGCGTACCGGGCAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTc > 1:1756249/1‑69 (MQ=255) cACGCGCAGCGTACCGGGCAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTc > 1:3326531/1‑69 (MQ=255) cACGCGCAGCGTACCGGGCAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTc > 1:605929/1‑69 (MQ=255) cACGCGCAGCGTACCGGGCAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTc > 1:2550294/1‑69 (MQ=255) cACGCGCAGCGTACCGGGCAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTc > 1:9102/1‑69 (MQ=255) cAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTTGTAttg < 1:1835434/69‑1 (MQ=255) cAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttg < 1:301540/69‑1 (MQ=255) cAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttg < 1:1777711/69‑1 (MQ=255) cAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttg < 1:1774300/69‑1 (MQ=255) cAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttg < 1:611475/69‑1 (MQ=255) cAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttg < 1:1123981/69‑1 (MQ=255) cAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttg < 1:949609/69‑1 (MQ=255) cAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCAATCCAGTCCATTTCGCCGTGGTAttg < 1:2281555/69‑1 (MQ=255) aaCTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTg > 1:1637824/1‑59 (MQ=255) aaCTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttgtttg > 1:2269928/1‑69 (MQ=255) aaCTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttgtttg > 1:1076416/1‑69 (MQ=255) aaCTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttgtttg > 1:1168397/1‑69 (MQ=255) aaCTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttgtttg > 1:3198122/1‑69 (MQ=255) aaCTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttgtttg > 1:3186475/1‑69 (MQ=255) aaCTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttgtttg > 1:1885459/1‑69 (MQ=255) aaCTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttgtttg > 1:209813/1‑69 (MQ=255) aaCTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTAttgtttg > 1:2226114/1‑69 (MQ=255) cTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGt > 1:2237174/1‑59 (MQ=255) cTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGt > 1:2813615/1‑59 (MQ=255) cTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGt > 1:3026730/1‑59 (MQ=255) cTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGt > 1:221195/1‑59 (MQ=255) gAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGcc > 1:649360/1‑69 (MQ=255) gcgcgcAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGc < 1:1801211/69‑1 (MQ=255) cgcgcAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCa < 1:1495813/69‑1 (MQ=255) cgcgcAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCa < 1:1103648/69‑1 (MQ=255) cgcgcAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCa < 1:888653/69‑1 (MQ=255) cgcgcAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCa < 1:847136/69‑1 (MQ=255) cgcgcAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCa < 1:608478/69‑1 (MQ=255) cgcgcAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCa < 1:3249761/69‑1 (MQ=255) cgcgcAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCa < 1:2176169/69‑1 (MQ=255) tACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt > 1:983713/1‑69 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:1794150/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:2113178/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:2136284/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:2905078/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:1962012/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:1143397/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:1958103/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:73458/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:3010836/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:1397191/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:50802/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:2214738/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:3167763/69‑1 (MQ=255) tACCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:3241118/69‑1 (MQ=255) aCCGTGACGCGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCt < 1:195257/68‑1 (MQ=255) gACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACt < 1:1331621/69‑1 (MQ=255) aCGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACt < 1:1447746/68‑1 (MQ=255) tGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATTCTTGCAGTTTGGGCTCGGACTCGCt < 1:1632606/69‑1 (MQ=255) tGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCt < 1:1327879/69‑1 (MQ=255) tGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCt < 1:691276/69‑1 (MQ=255) tGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCt < 1:1438826/69‑1 (MQ=255) tGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCt < 1:1094243/69‑1 (MQ=255) tGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCt < 1:2301068/69‑1 (MQ=255) | CTGATCACGCGCAGCGTACCGGGCAATAACTCATGAGGGCGAGCGCGCAGCATACCGTGACGTGCCATCCAGTCCATTTCGCCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCT > minE/2855500‑2855630 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |