Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,856,258 T→C 20.2% T177T (ACT→ACC yjeF → predicted carbohydrate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,856,2580TC20.2% 75.8 / 11.7 54T177T (ACT→ACCyjeFpredicted carbohydrate kinase
Reads supporting (aligned to +/- strand):  ref base T (22/21);  new base C (8/3);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 3.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTATTGCGCTGAAACCA  >  minE/2856199‑2856321
                                                           |                                                               
aaTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTAc                                                         >  1:728795/1‑68 (MQ=255)
aaTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTAc                                                         >  1:2281798/1‑68 (MQ=255)
aaTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTAc                                                         >  1:2148915/1‑68 (MQ=255)
aaTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTAc                                                         >  1:2453345/1‑68 (MQ=255)
aaTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTAc                                                         >  1:665332/1‑68 (MQ=255)
aaTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTAc                                                         >  1:3377064/1‑68 (MQ=255)
aaTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACg                                                        <  1:2283987/69‑1 (MQ=255)
aaTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACg                                                        <  1:1430144/69‑1 (MQ=255)
aaTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACg                                                        <  1:715750/69‑1 (MQ=255)
aaTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACg                                                        <  1:3005301/69‑1 (MQ=255)
 aTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACg                                                        <  1:938684/68‑1 (MQ=255)
 aTTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACg                                                        <  1:1381210/68‑1 (MQ=255)
    cccATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAg                                                    >  1:60958/1‑69 (MQ=255)
    cccATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAg                                                    >  1:1476236/1‑69 (MQ=255)
    cccATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAg                                                    >  1:1369430/1‑69 (MQ=255)
            gcgcCGATTGTGGCGGTTGATATCCCTTCCGGCCTGGTGGCTGAAACTGGCGCTACGCCAGGCGCGGTg                                            <  1:1493169/69‑1 (MQ=255)
            gcgcCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTg                                            <  1:2492914/69‑1 (MQ=255)
               ccGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATc                                         <  1:3132028/69‑1 (MQ=255)
               ccGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATc                                         <  1:3075717/69‑1 (MQ=255)
               ccGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATc                                         <  1:249248/69‑1 (MQ=255)
                cGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATc                                         <  1:1144937/68‑1 (MQ=255)
                          ggTTGATATCCCTTCCGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATca                              <  1:2085929/69‑1 (MQ=255)
                          ggTTGATATCCCTTCCGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATca                              <  1:1560561/69‑1 (MQ=255)
                          ggTTGATATCCCTTCCGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATca                              <  1:3174994/69‑1 (MQ=255)
                          ggTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATca                              <  1:2413889/69‑1 (MQ=255)
                          ggTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATca                              <  1:210930/69‑1 (MQ=255)
                          ggTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATca                              <  1:2548041/69‑1 (MQ=255)
                          ggTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATca                              <  1:1731322/69‑1 (MQ=255)
                          ggTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATca                              <  1:181512/69‑1 (MQ=255)
                             tGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCacac                           <  1:2564684/69‑1 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATc                                         >  1:2105390/1‑49 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:416763/1‑69 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:3364749/1‑69 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:3258970/1‑69 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:1520773/1‑69 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:3108754/1‑69 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:145180/1‑69 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:307571/1‑69 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:1283409/1‑69 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:862682/1‑69 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:2676173/1‑69 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:2637709/1‑69 (MQ=255)
                                   cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc                     >  1:2516643/1‑69 (MQ=255)
                                         cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACg                                     >  1:112088/1‑47 (MQ=37)
                                         cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt               >  1:403633/1‑69 (MQ=255)
                                         cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt               >  1:3270839/1‑69 (MQ=255)
                                         cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt               >  1:3081678/1‑69 (MQ=255)
                                         cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt               >  1:855165/1‑69 (MQ=255)
                                         cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt               >  1:2050734/1‑69 (MQ=255)
                                         cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt               >  1:236725/1‑69 (MQ=255)
                                         cGGCCTGCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt               >  1:435836/1‑69 (MQ=255)
                                                 tggctgAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTAt               <  1:3119278/61‑1 (MQ=255)
                                                      gAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTATTGCGCTGAAACCa  <  1:1721865/69‑1 (MQ=255)
                                                      gAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTATTGCGCTGAAACCa  <  1:3025660/69‑1 (MQ=255)
                                                           |                                                               
AATTCCCATCCTGCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTATTGCGCTGAAACCA  >  minE/2856199‑2856321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: