| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,856,279 | G→A | 28.1% | V184V (GTG→GTA) | yjeF → | predicted carbohydrate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,856,279 | 0 | G | A | 28.1% | 38.6 / 12.9 | 39 | V184V (GTG→GTA) | yjeF | predicted carbohydrate kinase |
| Reads supporting (aligned to +/- strand): ref base G (15/13); new base A (8/3); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTATTGCGCTGAAACCAGGCTTGCTCACTGGAAAAGCGCGGGA > minE/2856211‑2856347 | gcgcCGATTGTGGCGGTTGATATCCCTTCCGGCCTGGTGGCTGAAACTGGCGCTACGCCAGGCGCGGTg < 1:1493169/69‑1 (MQ=255)gcgcCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTg < 1:2492914/69‑1 (MQ=255) ccGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATc < 1:249248/69‑1 (MQ=255) ccGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATc < 1:3075717/69‑1 (MQ=255) ccGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATc < 1:3132028/69‑1 (MQ=255) cGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATc < 1:1144937/68‑1 (MQ=255) ggTTGATATCCCTTCCGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATca < 1:1560561/69‑1 (MQ=255) ggTTGATATCCCTTCCGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATca < 1:3174994/69‑1 (MQ=255) ggTTGATATCCCTTCCGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATca < 1:2085929/69‑1 (MQ=255) ggTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATca < 1:1731322/69‑1 (MQ=255) ggTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATca < 1:2548041/69‑1 (MQ=255) ggTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATca < 1:181512/69‑1 (MQ=255) ggTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATca < 1:2413889/69‑1 (MQ=255) ggTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATca < 1:210930/69‑1 (MQ=255) tGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCacac < 1:2564684/69‑1 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATc > 1:2105390/1‑49 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:307571/1‑69 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:2676173/1‑69 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:3364749/1‑69 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:416763/1‑69 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:862682/1‑69 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:2637709/1‑69 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:3108754/1‑69 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:3258970/1‑69 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:2516643/1‑69 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:1283409/1‑69 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:145180/1‑69 (MQ=255) cccTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCAc > 1:1520773/1‑69 (MQ=255) cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACg > 1:112088/1‑47 (MQ=37) cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt > 1:3081678/1‑69 (MQ=255) cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt > 1:3270839/1‑69 (MQ=255) cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt > 1:2050734/1‑69 (MQ=255) cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt > 1:403633/1‑69 (MQ=255) cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt > 1:855165/1‑69 (MQ=255) cGGCCTTCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt > 1:236725/1‑69 (MQ=255) cGGCCTGCTGGCTGAAACCGGCGCTACGCCAGGCGCGGTAATCAACGCCGATCACACCATCACTTTTAt > 1:435836/1‑69 (MQ=255) tggctgAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTAt < 1:3119278/61‑1 (MQ=255) gAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTATTGCGCTGAAACCa < 1:1721865/69‑1 (MQ=255) gAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTATTGCGCTGAAACCa < 1:3025660/69‑1 (MQ=255) ggCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTATTGCGCTGAAACCAGGCtt > 1:1479019/1‑68 (MQ=255) ggCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTATTGCGCTGAAACCAGGCTTg > 1:1786658/1‑69 (MQ=255) gATCAACGCCGATCACACCATCACTTTTATTGCGCTGAAACCAGGCTTGCTCACTGGAAAAGCGCGGGa < 1:1816412/69‑1 (MQ=255) | GCGCCGATTGTGGCGGTTGATATCCCTTCCGGCCTGCTGGCTGAAACTGGCGCTACGCCAGGCGCGGTGATCAACGCCGATCACACCATCACTTTTATTGCGCTGAAACCAGGCTTGCTCACTGGAAAAGCGCGGGA > minE/2856211‑2856347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |