| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,859,346 | C→T | 40.6% | S91S (AGC→AGT) | mutL → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,859,346 | 0 | C | T | 40.6% | 23.1 / 39.1 | 59 | S91S (AGC→AGT) | mutL | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base C (13/22); new base T (18/6); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.44e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
GTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCA > minE/2859294‑2859409 | gTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGTCTGGGCTTTCGCGGTg < 1:109382/69‑1 (MQ=255)gTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGTCTGGGCTTTCGCGGTg < 1:2859753/69‑1 (MQ=255)gTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGTCTGGGCTTTCGCGGTg < 1:1813651/69‑1 (MQ=255)gTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGTCTGGGCTTTCGCGGTg < 1:167439/69‑1 (MQ=255)gTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGTCTGGGCTTTCGCGGTg < 1:477559/69‑1 (MQ=255) tCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGTCTGGGCTTTCGCGGTg < 1:2847263/68‑1 (MQ=255) cATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGa < 1:2716746/68‑1 (MQ=255) cATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGa < 1:2293717/68‑1 (MQ=255) cATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAg > 1:1385042/1‑69 (MQ=255) gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCg > 1:1268085/1‑68 (MQ=255) gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCg > 1:3007808/1‑68 (MQ=255) gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCg > 1:1926160/1‑68 (MQ=255) gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCg > 1:1582871/1‑68 (MQ=255) gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCg > 1:1069780/1‑68 (MQ=255) gTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCg > 1:896506/1‑68 (MQ=255) aaaTCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGgcgc > 1:1446340/1‑60 (MQ=255) tGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGt < 1:1647570/67‑1 (MQ=255) ctcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTg > 1:1242402/1‑69 (MQ=255) ctcGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTg > 1:1910635/1‑69 (MQ=255) aGCCATTATCAGCCTGTGCTTTCTCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCt < 1:2775436/69‑1 (MQ=255) aGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGa < 1:3298165/65‑1 (MQ=255) aGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCt < 1:403987/69‑1 (MQ=255) aGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCt < 1:3323634/69‑1 (MQ=255) aGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCt < 1:3162531/69‑1 (MQ=255) cctttATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCa < 1:1506164/66‑1 (MQ=255) ccATTATCAGCCTGGGCTTTCGCTGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCa < 1:1106940/69‑1 (MQ=255) ccATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCa < 1:2812009/69‑1 (MQ=255) ccATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCa < 1:2622064/69‑1 (MQ=255) ccATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCa < 1:2944222/69‑1 (MQ=255) ccATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCa < 1:1777253/69‑1 (MQ=255) ccATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCa < 1:2406137/69‑1 (MQ=255) ccATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCa < 1:2117207/69‑1 (MQ=255) ccATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCa < 1:2061357/69‑1 (MQ=255) ccATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCa < 1:6081/69‑1 (MQ=255) ccATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCa < 1:1781180/69‑1 (MQ=255) ccATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGATTCCCGCCTGACGCTCa < 1:3340019/69‑1 (MQ=255) ttATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACtt < 1:2398249/69‑1 (MQ=255) ttATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACtt < 1:1168690/69‑1 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACtt > 1:972910/1‑68 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACtt > 1:479291/1‑68 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACtt > 1:2299066/1‑68 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:2648606/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:2566737/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:2541413/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:680930/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:2385618/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:3306242/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:2256897/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:1926655/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:1658183/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:456752/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:1625116/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:1559067/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:578349/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTc > 1:1547502/1‑69 (MQ=255) tATCAGTCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCAGCCTGACGCTCACTTc > 1:1243175/1‑69 (MQ=255) aTCAGCCTGGGCTTTCGCGGTGAGGGGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCa > 1:1322992/1‑69 (MQ=255) aTCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGt > 1:2456003/1‑42 (MQ=255) aTCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCa > 1:2644448/1‑69 (MQ=255) | GTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATTATCAGCCTGGGCTTTCGCGGTGAGGCGCTGGCGAGTATCAGTTCGGTTTCCCGCCTGACGCTCACTTCA > minE/2859294‑2859409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |