| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,863,467 | T→C | 26.3% | R378R (CGT→CGC) | hflX → | predicted GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,863,467 | 0 | T | C | 26.3% | 77.8 / 27.2 | 72 | R378R (CGT→CGC) | hflX | predicted GTPase |
| Reads supporting (aligned to +/- strand): ref base T (23/30); new base C (11/8); total (34/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.73e-01 | |||||||||||
GCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAG > minE/2863399‑2863535 | gCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGt > 1:1851809/1‑69 (MQ=255)gCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGt > 1:1862517/1‑69 (MQ=255)gCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGt > 1:1351995/1‑69 (MQ=255)gCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGt > 1:2417473/1‑69 (MQ=255) gAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGt < 1:1172859/69‑1 (MQ=255) gAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGt < 1:1955753/69‑1 (MQ=255) gAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGt < 1:2317449/69‑1 (MQ=255) gAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGt < 1:2690075/69‑1 (MQ=255) gAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGt < 1:1345896/69‑1 (MQ=255) gAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGt > 1:1370601/1‑54 (MQ=255) gAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGt > 1:2166537/1‑54 (MQ=255) gAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGt > 1:161298/1‑54 (MQ=255) gAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGt > 1:2728995/1‑54 (MQ=255) gAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGt > 1:3131461/1‑54 (MQ=255) gAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAACGGCGTCTgaga > 1:203482/1‑51 (MQ=255) gcagcaTACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCaa < 1:1430466/68‑1 (MQ=255) gcagcaTACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCaa < 1:2084463/68‑1 (MQ=255) gcaTACATTGCGTCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTa > 1:350162/1‑51 (MQ=39) gcaTACATTGCGTCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTAt > 1:971609/1‑52 (MQ=39) gcaTACATTGCGTCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTAt > 1:2437332/1‑52 (MQ=39) gcaTACATTGCGTCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTAt > 1:2691715/1‑52 (MQ=39) gcaTACATTGCGTCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTAt > 1:2281596/1‑52 (MQ=39) gcaTACATTGCGTCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTAt > 1:2739706/1‑52 (MQ=39) gcaTACATTGCGTCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTAt > 1:526767/1‑52 (MQ=39) gcaTACATTGCGTCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTAt > 1:1715876/1‑52 (MQ=39) gcaTACATTGCGTCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTAt > 1:1355996/1‑52 (MQ=39) gcaTACATTGCGTCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTAt > 1:1043125/1‑52 (MQ=39) gcaTACATTGCGTCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTAt > 1:1628336/1‑52 (MQ=39) gcaTACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGa < 1:2625580/69‑1 (MQ=255) caTACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGa < 1:589439/68‑1 (MQ=255) caTACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGa < 1:3191018/68‑1 (MQ=255) tACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGaaaa < 1:2019209/69‑1 (MQ=255) cATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGaaaaa > 1:2894471/1‑68 (MQ=255) cATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAag > 1:459042/1‑69 (MQ=255) cATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAag > 1:447038/1‑69 (MQ=255) cATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAag > 1:757783/1‑69 (MQ=255) cATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAag > 1:3346106/1‑69 (MQ=255) cATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAag > 1:3317135/1‑69 (MQ=255) cATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAag > 1:318484/1‑69 (MQ=255) cATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAag > 1:2931011/1‑69 (MQ=255) cATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAag > 1:1312595/1‑69 (MQ=255) gCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg < 1:2848947/69‑1 (MQ=255) gCGTCTGCCACAGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg > 1:1431696/1‑69 (MQ=255) tCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtgga < 1:3261932/68‑1 (MQ=255) tCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtgga < 1:2226142/68‑1 (MQ=255) tCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtgga < 1:2181522/68‑1 (MQ=255) tCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtgga < 1:1943021/68‑1 (MQ=255) tCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtgga < 1:634910/68‑1 (MQ=255) tCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtgga < 1:803378/68‑1 (MQ=255) tCTGCCGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtgga < 1:849140/68‑1 (MQ=255) tCTGACGCCGCAGGAAGGGCGCCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtgga < 1:229727/68‑1 (MQ=255) ccGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATggaggag < 1:125611/69‑1 (MQ=255) ccGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATggaggag < 1:2790894/69‑1 (MQ=255) ccGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATggaggag < 1:546713/69‑1 (MQ=255) ccGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATggaggag < 1:2578659/69‑1 (MQ=255) aaGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATggaggag < 1:1317458/62‑1 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGaa > 1:1673478/1‑43 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg > 1:1892409/1‑51 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg < 1:104012/51‑1 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg < 1:1625643/51‑1 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg < 1:3333273/51‑1 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg < 1:3318150/51‑1 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg > 1:2212987/1‑51 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg < 1:1038482/51‑1 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg < 1:3314114/51‑1 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg > 1:3154337/1‑51 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg > 1:1183226/1‑51 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg > 1:2965185/1‑51 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg < 1:2940876/51‑1 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg < 1:2644809/51‑1 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg < 1:224288/51‑1 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGtg < 1:2807002/51‑1 (MQ=255) aGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATACAAAAAGAGtg > 1:1742270/1‑51 (MQ=255) gCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATggaggag < 1:2491115/58‑1 (MQ=255) gCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATggaggag < 1:1275195/58‑1 (MQ=255) cGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATggaggag < 1:2235929/57‑1 (MQ=255) tCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAg < 1:2872836/69‑1 (MQ=255) tCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAg < 1:541108/69‑1 (MQ=255) tCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAg < 1:1621776/69‑1 (MQ=255) tCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAg < 1:1668146/69‑1 (MQ=255) tCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAg < 1:1556007/69‑1 (MQ=255) tCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAg < 1:3341340/69‑1 (MQ=255) | GCTTTGACGGAGCGGCTTTCCGGCGAGGTGGCGCAGCATACATTGCGTCTGCCACCGCAGGAAGGGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAG > minE/2863399‑2863535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |