| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,869,590 | T→C | 39.3% | G425G (GGT→GGC) | rnr → | exoribonuclease R, RNase R |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,869,590 | 0 | T | C | 39.3% | 56.6 / 84.5 | 122 | G425G (GGT→GGC) | rnr | exoribonuclease R, RNase R |
| Reads supporting (aligned to +/- strand): ref base T (30/44); new base C (15/33); total (45/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-04 | |||||||||||
Only 100 of 123 total aligned reads displayed.
TAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGT > minE/2869527‑2869653 | tAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTggg < 1:1582199/67‑1 (MQ=255)tAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTggg < 1:783846/67‑1 (MQ=255)tAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTggg < 1:2302205/67‑1 (MQ=255)tAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTggg < 1:614968/67‑1 (MQ=255)tAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTggg < 1:2530817/67‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:1222832/68‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:2703456/68‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:137895/68‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:2879571/68‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:2951362/68‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:3287603/68‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:3373778/68‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:2050331/68‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:1237159/68‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:695021/68‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:793540/68‑1 (MQ=255) cATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:793702/68‑1 (MQ=255) tcGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCAt > 1:2291832/1‑66 (MQ=255) tcGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCAt > 1:2299101/1‑66 (MQ=255) tcGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCAt > 1:2814573/1‑66 (MQ=255) tcGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCAt > 1:216352/1‑66 (MQ=255) gagTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGAtctc < 1:713458/59‑1 (MQ=255) tGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCgaag < 1:150396/69‑1 (MQ=255) tGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCgaag < 1:894516/69‑1 (MQ=255) aCCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGa > 1:2424533/1‑69 (MQ=255) aCCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGa > 1:1926868/1‑69 (MQ=255) aCCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGa > 1:3038893/1‑69 (MQ=255) aCCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGa > 1:592728/1‑69 (MQ=255) aCCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGa > 1:823712/1‑69 (MQ=255) aCCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGa > 1:1452588/1‑69 (MQ=255) aCCTCTATAAAGTCCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGa > 1:1716197/1‑69 (MQ=255) taAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGaagaag > 1:3320427/1‑59 (MQ=255) taAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCa > 1:1308331/1‑68 (MQ=255) taAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCa > 1:1588773/1‑68 (MQ=255) taAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAt > 1:353624/1‑69 (MQ=255) taAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAt > 1:2884680/1‑69 (MQ=255) taAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAt > 1:3205369/1‑69 (MQ=255) taAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAt > 1:1016558/1‑69 (MQ=255) taAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAt > 1:1021067/1‑69 (MQ=255) taAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAt > 1:1988325/1‑69 (MQ=255) aaGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCa < 1:3307441/66‑1 (MQ=255) aaGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCa > 1:716173/1‑66 (MQ=255) aaGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCa < 1:3387492/66‑1 (MQ=255) aaGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtt > 1:1737831/1‑68 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:2548154/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:765191/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:1038790/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:1439690/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:307993/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:1065382/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:268170/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:2638645/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:3165069/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:699137/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:1859578/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:2153271/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:1963595/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:1090545/68‑1 (MQ=255) gTGCTGGATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGAGAAGAAGCGAAGTTCAtttt < 1:112503/68‑1 (MQ=255) gCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCaa < 1:1269001/69‑1 (MQ=255) cTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCa < 1:2612216/61‑1 (MQ=255) gATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACg > 1:736279/1‑67 (MQ=255) gATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACg > 1:2886604/1‑67 (MQ=255) gATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGc > 1:1743196/1‑68 (MQ=255) gATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGc > 1:3007625/1‑68 (MQ=255) gATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGc > 1:3023564/1‑68 (MQ=255) gATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGc > 1:2709341/1‑68 (MQ=255) gATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGc > 1:2632937/1‑68 (MQ=255) gATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGc > 1:2135834/1‑68 (MQ=255) gATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGc > 1:1824635/1‑68 (MQ=255) gATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGc > 1:2386613/1‑68 (MQ=255) gATAAAGCCCGTGAAGAACGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGc > 1:499615/1‑68 (MQ=255) gCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCaa < 1:3179664/59‑1 (MQ=255) ccGTGAAGAACGCGGTGGGATCTCATTTGAGAGCgaag < 1:3254070/38‑1 (MQ=255) ccGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCg < 1:1069960/69‑1 (MQ=255) ccGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCg < 1:735143/69‑1 (MQ=255) ccGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCg < 1:1139742/69‑1 (MQ=255) ccGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCg < 1:694659/69‑1 (MQ=255) ccGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCg < 1:604104/69‑1 (MQ=255) ccGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCg < 1:3159869/69‑1 (MQ=255) ccGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCg < 1:1307599/69‑1 (MQ=255) aagaaCGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTg < 1:1551982/69‑1 (MQ=255) aagaaCGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTg > 1:2082315/1‑69 (MQ=255) aagaaCGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTg > 1:3324846/1‑69 (MQ=255) aagaaCGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTg < 1:1302235/69‑1 (MQ=255) aagaaCGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTg > 1:1687435/1‑69 (MQ=255) gaaCGCGGTGGGATCTTATTTGAGAGCGAAGAAGCGAAGTTCa < 1:219570/43‑1 (MQ=255) gaaCGCGGCGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttt < 1:123917/47‑1 (MQ=255) cgctGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACg < 1:1246257/68‑1 (MQ=255) cgcgGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACg < 1:1969058/68‑1 (MQ=255) cgcgGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACg < 1:1745516/68‑1 (MQ=255) cgcgGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACg < 1:1808144/68‑1 (MQ=255) cgcgGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACg < 1:1663973/68‑1 (MQ=255) cgcgGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACg < 1:1189467/68‑1 (MQ=255) cgcgGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACg < 1:374970/68‑1 (MQ=255) cgcgGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACg < 1:2355507/68‑1 (MQ=255) cgcgGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACg < 1:2497491/68‑1 (MQ=255) cgcgGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTAAACGCCGTATTGAACGt < 1:1463388/69‑1 (MQ=255) cgcgGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTAAACGCCGTATTGAACGt < 1:2885151/69‑1 (MQ=255) gcggcgGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtttg < 1:2300780/43‑2 (MQ=255) | TAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGT > minE/2869527‑2869653 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |