| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,869,908 | T→C | 51.3% | D531D (GAT→GAC) | rnr → | exoribonuclease R, RNase R |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,869,908 | 0 | T | C | 51.3% | 2.1 / 70.6 | 80 | D531D (GAT→GAC) | rnr | exoribonuclease R, RNase R |
| Reads supporting (aligned to +/- strand): ref base T (23/16); new base C (27/14); total (50/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01 | |||||||||||
CGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAA > minE/2869845‑2869968 | cGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGa < 1:1098294/69‑1 (MQ=255)cGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGa < 1:527836/69‑1 (MQ=255)cGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGa < 1:1569827/69‑1 (MQ=255) aCAAGCCGTAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAAtgc > 1:1912181/1‑69 (MQ=255) aCAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAAtgc > 1:2391769/1‑69 (MQ=255) aCAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAAtg > 1:1376974/1‑68 (MQ=255) aCAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAAtg > 1:2813924/1‑68 (MQ=255) aCAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCT‑ATGCAGAAAtgc > 1:3307698/1‑68 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc > 1:1811770/1‑69 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc > 1:976339/1‑69 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc < 1:1931885/69‑1 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc > 1:2022043/1‑69 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc < 1:2086992/69‑1 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc > 1:2414707/1‑69 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc > 1:260109/1‑69 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc > 1:2783095/1‑69 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc < 1:3182847/69‑1 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc < 1:739809/69‑1 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc > 1:1096453/1‑69 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc > 1:152123/1‑69 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc > 1:1548988/1‑69 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc < 1:1529109/69‑1 (MQ=255) ggAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCATGATGCAGAAATGCTGCAAAc < 1:2976425/69‑1 (MQ=255) gAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc < 1:3128916/68‑1 (MQ=255) gAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc < 1:713671/68‑1 (MQ=255) ccGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCAtg > 1:2952294/1‑69 (MQ=255) ccGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCAtg > 1:158794/1‑69 (MQ=255) ccGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCAtg > 1:585100/1‑69 (MQ=255) aGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAAc < 1:488842/49‑1 (MQ=255) aGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGa < 1:1770704/69‑1 (MQ=255) aGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGa < 1:1348934/69‑1 (MQ=255) aGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGa < 1:1991838/69‑1 (MQ=255) gTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCg < 1:1472306/67‑1 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:555200/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:1221385/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:71240/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:3368071/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:1308716/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:3197586/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:730785/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:732646/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:3121745/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:1219323/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:1137995/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:2895805/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:1408659/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:2753319/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:2655907/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:1908050/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:2604851/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:1476130/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:2595625/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:150032/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:214256/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:1985678/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:2039253/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:210165/1‑43 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgct > 1:3362732/1‑42 (MQ=255) ggTTGCCGATCGTCCTGACGCAGAAAAGCTGCAAACCAtgctg > 1:2909961/1‑43 (MQ=38) ggTTACCGATCGTCCTGACGCAGAAATGCTGCAAACCAtgctg > 1:488948/1‑43 (MQ=38) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:448143/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:1866208/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:598123/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:1998089/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:1944196/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:1656410/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:3359141/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:331715/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:2255924/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:233215/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:3158931/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:2400006/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:2503121/69‑1 (MQ=255) tGCCGATCGTCCTGACGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGa < 1:2756810/69‑1 (MQ=255) cGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCg > 1:2278747/1‑54 (MQ=255) cGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGaa > 1:441771/1‑69 (MQ=255) cGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGaa > 1:1998862/1‑69 (MQ=255) cGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGaa > 1:710030/1‑69 (MQ=255) cGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGaa > 1:2655351/1‑69 (MQ=255) cGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGa > 1:211166/1‑68 (MQ=255) | CGGTAACAAGCCGGAACCGCGTGACTACGCGGAGCTGCTGGAGTCGGTTGCCGATCGTCCTGATGCAGAAATGCTGCAAACCATGCTGCTGCGCTCGATGAAACAGGCGATTTACGATCCAGAA > minE/2869845‑2869968 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |