| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,872,333 | G→A | 28.1% | R195Q (CGG→CAG) ‡ | ulaF → | L‑ribulose 5‑phosphate 4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,872,333 | 0 | G | A | 28.1% | 99.9 / 37.5 | 92 | R195Q (CGG→CAG) ‡ | ulaF | L‑ribulose 5‑phosphate 4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base G (33/33); new base A (19/7); total (52/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.16e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-01 | |||||||||||
CACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACA > minE/2872270‑2872397 | cACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt > 1:2180039/1‑69 (MQ=255)cACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt > 1:2332336/1‑69 (MQ=255)cACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt > 1:1406114/1‑69 (MQ=255)cACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt > 1:75472/1‑69 (MQ=255)cACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt > 1:2730504/1‑69 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:2669394/1‑68 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:2082913/1‑68 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:507081/1‑68 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:373853/1‑68 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:2534476/1‑68 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:2620190/1‑68 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:2622199/1‑68 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:684853/1‑68 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:1253460/1‑68 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:3082803/1‑68 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:325488/1‑68 (MQ=255) tAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa > 1:532612/1‑68 (MQ=255) aCGCGGTGGTGATGGAAGAAGTGGCTAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAAt < 1:1541384/68‑1 (MQ=255) aCGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAAt < 1:632898/68‑1 (MQ=255) aCGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAAt < 1:1758937/68‑1 (MQ=255) aCGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAAt < 1:586113/68‑1 (MQ=255) aCGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAAt < 1:2335985/68‑1 (MQ=255) aCGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAAt < 1:1159805/68‑1 (MQ=255) gtggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATc < 1:3210881/69‑1 (MQ=255) gtggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATc < 1:3264715/69‑1 (MQ=255) gtggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATc < 1:385272/69‑1 (MQ=255) gtggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATc < 1:52835/69‑1 (MQ=255) gtggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATc < 1:898143/69‑1 (MQ=255) gtggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATc < 1:1435984/69‑1 (MQ=255) gtggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATc < 1:464791/69‑1 (MQ=255) gtggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATc < 1:1896392/69‑1 (MQ=255) gtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGAc > 1:1961525/1‑69 (MQ=255) gtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGAc > 1:874158/1‑69 (MQ=255) aTGGAAGAAGTGGCGAAAATGGCGGGGATTGCCCGCGGCATTAACCCACAACTCAAt < 1:67675/57‑1 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCGCGTAGTCTTAACCCACAACTGAATCACATCGACAGCt < 1:760691/68‑1 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCt < 1:1432560/68‑1 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCt < 1:426996/68‑1 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCt < 1:1667743/68‑1 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCt < 1:1527108/68‑1 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCt < 1:1482122/68‑1 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCtt > 1:1236352/1‑69 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCtt > 1:3194032/1‑69 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCtt > 1:2890721/1‑69 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCtt > 1:2617375/1‑69 (MQ=255) ggAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCtt > 1:319836/1‑69 (MQ=255) agaagTGGCGAAAATGGCGTTGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt < 1:511440/69‑1 (MQ=255) agaagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt < 1:2115037/69‑1 (MQ=255) gTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtgatg < 1:2280354/69‑1 (MQ=255) gTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtgatg < 1:2393675/69‑1 (MQ=255) tGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtgatg < 1:1786254/68‑1 (MQ=255) aaaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCt < 1:865353/55‑1 (MQ=255) aaaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATaaa < 1:2818719/69‑1 (MQ=255) aaaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATaaa < 1:335799/69‑1 (MQ=255) aaaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATaaa < 1:343198/69‑1 (MQ=255) aaaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATaaa < 1:860214/69‑1 (MQ=255) aaaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATaaa < 1:383840/69‑1 (MQ=255) aaaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATaaa < 1:1214866/69‑1 (MQ=255) aaaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATaaa < 1:1939814/69‑1 (MQ=255) aaaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATaaa < 1:1985994/69‑1 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCt > 1:1961135/1‑54 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATaaa > 1:353862/1‑68 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:457761/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:548105/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:610579/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:1003361/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:1771166/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:1871950/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:800711/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:1906182/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:2029631/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:2083952/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:2168973/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:2355464/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:2452573/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:2532198/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:2614956/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:2834302/1‑69 (MQ=255) aaaTGGCGTGGATTGCGCGTAGTATTAACCCACAACTGAATCACATCGACAGCTTCCTGATGAATAAac > 1:3114208/1‑69 (MQ=255) aaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATaaa < 1:1559420/68‑1 (MQ=255) ggCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACtt < 1:94557/69‑1 (MQ=255) ggCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACtt < 1:2174452/69‑1 (MQ=255) ggCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACtt < 1:1765879/69‑1 (MQ=255) gCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTc > 1:2859824/1‑69 (MQ=255) gATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGc > 1:3074752/1‑68 (MQ=255) gATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGc > 1:2852120/1‑68 (MQ=255) gATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGc > 1:764542/1‑68 (MQ=255) gATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGc > 1:827475/1‑68 (MQ=255) gATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGc > 1:843800/1‑68 (MQ=255) gATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGc > 1:1582685/1‑68 (MQ=255) gATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGc > 1:1456525/1‑68 (MQ=255) gATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGc > 1:1092255/1‑68 (MQ=255) aTTGCCCGCGGCATTAACCCACAACTCAATCACATc < 1:1808642/36‑1 (MQ=255) cgcgGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGGGTAAaca < 1:1882870/68‑1 (MQ=255) cgcgGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAaca < 1:3036452/68‑1 (MQ=255) cgcgGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAaca < 1:2776126/68‑1 (MQ=255) ggCATTAACCCACAACTCAATCACATCGACAGCTTCCtgatg < 1:3398463/42‑1 (MQ=255) | CACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACA > minE/2872270‑2872397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |