BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,872,696	A→G	17.5%	N46N (AAT→AAC)	yjfY ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,872,696	0	A	G	17.5%	160.5 / 21.2	102	N46N (AAT→AAC)	yjfY	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (38/46); new base G (5/13); total (43/59)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATT  >  minE/2872630‑2872761

gtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAtt                                                                 >  1:1190805/1‑69 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAtt                                                                 >  1:1242241/1‑69 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAtt                                                                 >  1:1341653/1‑69 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAtt                                                                 >  1:1337689/1‑69 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAt                                                                  >  1:291542/1‑68 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGtt                                                                 <  1:2150991/69‑1 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGtt                                                                 <  1:928428/69‑1 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGtt                                                                 <  1:1191190/69‑1 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGtt                                                                 <  1:2414603/69‑1 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGtt                                                                 <  1:490493/69‑1 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGtt                                                                 <  1:2565638/69‑1 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGtt                                                                 <  1:2725384/69‑1 (MQ=255)

gtaCGTTGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGtt                                                                 <  1:3077685/69‑1 (MQ=255)

gtaCGTTGAGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGtt                                                                 <  1:629691/69‑1 (MQ=255)

gtaAGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAtt                                                                 >  1:93374/1‑69 (MQ=255)

 taCGTTGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGtt                                                                 <  1:1647279/68‑1 (MQ=255)

 taCGTTGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGtt                                                                 <  1:1963330/68‑1 (MQ=255)

 taCGTTGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCCCAGTGGCGAAGtt                                                                 <  1:242846/68‑1 (MQ=255)

   cGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGaaa                                                                   >  1:3286930/1‑64 (MQ=255)

   cGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAt                                                                  >  1:510367/1‑65 (MQ=255)

   cGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtg                                                              >  1:3265471/1‑69 (MQ=255)

   cGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtg                                                              >  1:2709407/1‑69 (MQ=255)

   cGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtg                                                              >  1:3372267/1‑69 (MQ=255)

   cGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtg                                                              >  1:2181839/1‑69 (MQ=255)

   cGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtg                                                              >  1:1057561/1‑69 (MQ=255)

   cGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtg                                                              >  1:2110359/1‑69 (MQ=255)

     ttGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGTTAtga                                                             >  1:1045536/1‑68 (MQ=255)

     ttGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGTTAtga                                                             >  1:2597171/1‑68 (MQ=255)

     ttGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGTTAtga                                                             >  1:2177438/1‑68 (MQ=255)

     ttGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGTTAtga                                                             >  1:450233/1‑68 (MQ=255)

     ttGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGTGCTTCACTCTCAGTGGCGAAGTTAtga                                                             >  1:3006730/1‑68 (MQ=255)

      tGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgatt                                                           <  1:863004/69‑1 (MQ=255)

      tGCGCCTTGCGCATCTGTCTCTTCATTTAATGCCTGACGAGCTTCACTCTCAGTGGCTAAGtt                                                                 <  1:2232629/63‑1 (MQ=255)

                gcATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:3013352/68‑1 (MQ=255)

                gcATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:2585095/68‑1 (MQ=255)

                gcATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:2320917/68‑1 (MQ=255)

                gcATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:1428602/68‑1 (MQ=255)

                gcATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:2923173/68‑1 (MQ=255)

                gcATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:2133914/68‑1 (MQ=255)

                gcATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:1226709/68‑1 (MQ=255)

                gcATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:59680/68‑1 (MQ=255)

                gcATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:1179133/68‑1 (MQ=255)

                gcATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:117831/68‑1 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:1698789/63‑1 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:1208835/63‑1 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:2720772/63‑1 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:66405/63‑1 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                  <  1:246409/63‑1 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                             >  1:944398/1‑68 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                             >  1:1283187/1‑68 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                             >  1:2908568/1‑68 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                             >  1:2901691/1‑68 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                             >  1:732857/1‑68 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                             >  1:1644384/1‑68 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                             >  1:1747726/1‑68 (MQ=255)

                     tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                             >  1:2318403/1‑68 (MQ=255)

                         tcttcATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAtt                                                                 >  1:567052/1‑44 (MQ=255)

                         tcttcATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAAtt                                                                 >  1:1311240/1‑44 (MQ=255)

                               tttAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTg                                   <  1:2617344/68‑1 (MQ=255)

                               tttAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTg                                   <  1:1530246/68‑1 (MQ=255)

                               tttAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTg                                   <  1:379325/68‑1 (MQ=255)

                               tttAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTg                                   <  1:1223476/68‑1 (MQ=255)

                               tttAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTg                                   <  1:2974835/68‑1 (MQ=255)

                                 tAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGcac                                <  1:1915180/69‑1 (MQ=255)

                                 tAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGcac                                <  1:2576697/69‑1 (MQ=255)

                                 tAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGcac                                <  1:3433522/69‑1 (MQ=255)

                                 tAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGcac                                <  1:1484004/69‑1 (MQ=255)

                                 tAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGaac                                <  1:2653189/69‑1 (MQ=255)

                                     gCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTg                                   <  1:1677750/62‑1 (MQ=255)

                                      ccTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCg                            >  1:1856157/1‑68 (MQ=255)

                                      ccTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCg                            >  1:1718509/1‑68 (MQ=255)

                                      ccTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCg                            >  1:1939841/1‑68 (MQ=255)

                                      ccTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCg                            >  1:2033400/1‑68 (MQ=255)

                                      ccTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCg                            >  1:2681614/1‑68 (MQ=255)

                                      ccTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCg                            >  1:2719108/1‑68 (MQ=255)

                                         gACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCa                        <  1:2104285/69‑1 (MQ=255)

                                         gACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCa                        <  1:793926/69‑1 (MQ=255)

                                         gACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCa                        <  1:1912290/69‑1 (MQ=255)

                                         gACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCa                        <  1:1338513/69‑1 (MQ=255)

                                         gACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCa                        <  1:1646780/69‑1 (MQ=255)

                                             tGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAtt                    <  1:3166054/69‑1 (MQ=255)

                                             tGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAtt                    <  1:121751/69‑1 (MQ=255)

                                             tGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAtt                    <  1:468866/69‑1 (MQ=255)

                                             tGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTACATAtt                    <  1:2465248/69‑1 (MQ=255)

                                             tGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTACATAtt                    <  1:2147/69‑1 (MQ=255)

                                              gCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAtt                    <  1:1931902/68‑1 (MQ=255)

                                                  cGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTgg               >  1:660633/1‑69 (MQ=255)

                                                             gCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGtt     >  1:518434/1‑68 (MQ=255)

                                                             gCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGtt     >  1:3375688/1‑68 (MQ=255)

                                                             gCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGtt     >  1:207467/1‑68 (MQ=255)

                                                             gCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGtt     >  1:864089/1‑68 (MQ=255)

                                                             gCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGtt     >  1:1494775/1‑68 (MQ=255)

                                                               gAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt  <  1:1974808/69‑1 (MQ=255)

                                                               gAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt  <  1:1370633/69‑1 (MQ=255)

                                                               gAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt  <  1:2220040/69‑1 (MQ=255)

                                                               gAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt  <  1:1863005/69‑1 (MQ=255)

                                                               gAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt  <  1:923058/69‑1 (MQ=255)

                                                               gAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt  <  1:185145/69‑1 (MQ=255)

                                                               gAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt  <  1:3056749/69‑1 (MQ=255)

                                                                aaaTTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt  <  1:377826/68‑1 (MQ=255)

GTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATT  >  minE/2872630‑2872761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`