| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,878,535 | T→C | 35.3% | G210G (GGT→GGC) | cycA → | D‑alanine/D‑serine/glycine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,878,535 | 0 | T | C | 35.3% | 39.8 / 36.8 | 65 | G210G (GGT→GGC) | cycA | D‑alanine/D‑serine/glycine transporter |
| Reads supporting (aligned to +/- strand): ref base T (8/34); new base C (14/9); total (22/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTAC > minE/2878470‑2878603 | tCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:2394616/69‑1 (MQ=255)tCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:1684022/69‑1 (MQ=255)tCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:177210/69‑1 (MQ=255)tCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:2897973/69‑1 (MQ=255)tCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:2851234/69‑1 (MQ=255)tCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:2540032/69‑1 (MQ=255) cACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTaa > 1:463178/1‑69 (MQ=255) cACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTaa > 1:1943691/1‑69 (MQ=255) cACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTaa > 1:1268271/1‑69 (MQ=255) cACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTaa > 1:1867537/1‑69 (MQ=255) cGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGt < 1:1660343/68‑1 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGCCGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:106567/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:1900426/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:1869252/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:1834548/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:222567/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:3023958/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:1083427/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:1017319/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:1262167/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:447825/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:3252109/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:3122264/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgcg > 1:1526883/1‑67 (MQ=255) cGACCGGTGTGGAAGCATCATTTGCACATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGCTTAAgc > 1:2180391/1‑67 (MQ=255) gACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGt < 1:2525710/67‑1 (MQ=255) gAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGc > 1:3390497/1‑68 (MQ=255) gAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGc > 1:2242116/1‑68 (MQ=255) gAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGc > 1:2508767/1‑68 (MQ=255) aTTCGCGCATTTGTTGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:45246/46‑1 (MQ=255) aTTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGtta < 1:2350335/45‑2 (MQ=255) aTTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:2862187/46‑1 (MQ=255) aTTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:104689/46‑1 (MQ=255) aTTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:842079/46‑1 (MQ=255) aTTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:968055/46‑1 (MQ=255) aTTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:1072870/46‑1 (MQ=255) aTTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTa < 1:1504640/46‑1 (MQ=255) cgcgCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGAt > 1:2592278/1‑69 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:2241476/69‑1 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:999063/69‑1 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:1221632/69‑1 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:1290777/69‑1 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:3366104/69‑1 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:3130887/69‑1 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:23491/69‑1 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:1899721/69‑1 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:2022135/69‑1 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:2175665/69‑1 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:2668213/69‑1 (MQ=255) gAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGGTTTCGc < 1:99118/69‑1 (MQ=255) aaTGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGc < 1:31310/68‑1 (MQ=255) tGACGGCGGCTGGTTCCCGAAAGGCTTAAGCGGCTTCTTTGCCGGATTCCAGATAg < 1:430770/56‑1 (MQ=255) tGACGGCGGCTGGTTCCCGAAAGGCTTAAGCGGCTTCTTTGCCGGATTCCAGATAg < 1:987771/56‑1 (MQ=255) tGACGGCGGCTGGTTCCCGAAAGGCTTAAGCGGCTTCTTTGCCGGATTCCAGATAg < 1:1048044/56‑1 (MQ=255) tGACGGCGGCTGGTTCCCGAAAGGCTTAAGCGGCTTCTTTGCCGGATTCCAGATAg < 1:2534636/56‑1 (MQ=255) tGACGGCGGCTGGTTCCCGAAAGGCTTAAGCGGCTTCTTTGCCGGATTCCAGATAg < 1:1698337/56‑1 (MQ=255) tGACGGCGGCTGGTTCCCGAAAGGCTTAAGCGGCTTCTTTGCCGGATTCCAGATAg < 1:3434927/56‑1 (MQ=255) tGACGGCGGCTGGTTCCCGAAAGGCTTAAGCGGCTTCTTTGCCGGATTCCAGATAg < 1:3279041/56‑1 (MQ=255) tGACGGCGGCTGGTTCCCGAAAGGCTTAAGCGGCTTCTTTGCCGGATTCCAGATAg < 1:15018/56‑1 (MQ=255) tGACGGCGGCTGGTTCCCGAAAGGCTTAAGCGGCTTCTTTGCCGGATTCCAGATAg < 1:1974244/56‑1 (MQ=255) cggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTggg < 1:1254460/69‑1 (MQ=255) cggcTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTggg < 1:757826/69‑1 (MQ=255) ggTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTg < 1:64707/69‑1 (MQ=255) ggTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTg < 1:3230824/69‑1 (MQ=255) ggTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTg < 1:3027327/69‑1 (MQ=255) ggTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTACAGATAGCGGTTTTCGCTTTCGTGGGGATTg < 1:1310653/69‑1 (MQ=255) tttAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTac < 1:787347/69‑1 (MQ=255) | TCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGACGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCTTCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTGGGGATTGAGCTGGTAGGTAC > minE/2878470‑2878603 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |