| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,879,390 | C→T | 18.7% | L212L (CTG→CTA) | ytfE ← | predicted regulator of cell morphogenesis and cell wall metabolism |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,879,390 | 0 | C | T | 18.7% | 175.0 / 25.0 | 111 | L212L (CTG→CTA) | ytfE | predicted regulator of cell morphogenesis and cell wall metabolism |
| Reads supporting (aligned to +/- strand): ref base C (32/58); new base T (12/9); total (44/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
ATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATT > minE/2879326‑2879443 | aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:841510/1‑69 (MQ=255)aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:36363/1‑69 (MQ=255)aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:475715/1‑69 (MQ=255)aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:3304868/1‑69 (MQ=255)aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:3265808/1‑69 (MQ=255)aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:3200889/1‑69 (MQ=255)aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:1551885/1‑69 (MQ=255)aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:915685/1‑69 (MQ=255)aTCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTa > 1:2911271/1‑69 (MQ=255) cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCTCGCGCGGGAATAGTAc < 1:3131044/64‑1 (MQ=255) cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGt > 1:2253824/1‑69 (MQ=255) cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGt > 1:2236991/1‑69 (MQ=255) cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:1281040/64‑1 (MQ=255) cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:2366529/64‑1 (MQ=255) cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:2639575/64‑1 (MQ=255) cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:1532088/64‑1 (MQ=255) cTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:2103278/64‑1 (MQ=255) ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:978444/1‑69 (MQ=255) ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:2820051/1‑69 (MQ=255) ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:552790/1‑69 (MQ=255) ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:1205638/1‑69 (MQ=255) ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:860433/1‑69 (MQ=255) ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:2337038/1‑69 (MQ=255) ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:867696/1‑69 (MQ=255) ggATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGttt > 1:2486267/1‑69 (MQ=255) ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGTGAACAGTACATTGTTTTCCa < 1:1751567/69‑1 (MQ=255) ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa < 1:2781767/69‑1 (MQ=255) ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa < 1:1979121/69‑1 (MQ=255) ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa < 1:3212421/69‑1 (MQ=255) ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa < 1:159193/69‑1 (MQ=255) ggCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCa < 1:2439673/69‑1 (MQ=255) gcAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGa > 1:1993383/1‑68 (MQ=255) gcAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGa > 1:669422/1‑68 (MQ=255) gcAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGa > 1:1220018/1‑68 (MQ=255) cAATGCCATCCAGCTTTTAGATAACTCACCCGCCAGCGCGCGCGGGAATAGTAc < 1:876220/54‑1 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCCCGCGCGCGCGGGAATAGTACATTGt > 1:167874/1‑57 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCCCGCGCGCGCGGGAATAGTACATTGt > 1:94400/1‑57 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:2712921/1‑57 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:1163450/1‑57 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:1311631/1‑57 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:3033353/1‑57 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:3076915/1‑57 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:1548665/1‑57 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:2162992/1‑57 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:737114/1‑57 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGCGGGAATAGTACATTGt > 1:656970/1‑57 (MQ=255) aTGCCATCCAGCTTTTAGATCACTCACCCGCAAGCGCGCGCGGGAATAGTACATTGt > 1:1444208/1‑57 (MQ=255) ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:762438/1‑69 (MQ=255) ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:320997/1‑69 (MQ=255) ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:3290534/1‑69 (MQ=255) ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:2731143/1‑69 (MQ=255) ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:1059265/1‑69 (MQ=255) ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:1056003/1‑69 (MQ=255) ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:1636119/1‑69 (MQ=255) ccATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgt > 1:2212209/1‑69 (MQ=255) aTCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgtg < 1:1089570/68‑1 (MQ=255) aTCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgtg < 1:1672241/68‑1 (MQ=255) aTCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgtg < 1:1669579/68‑1 (MQ=255) aTCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGAtgtg < 1:1527074/68‑1 (MQ=255) cAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACt > 1:2708597/1‑59 (MQ=255) cAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATc < 1:1525886/68‑1 (MQ=255) aTCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtca < 1:880771/68‑1 (MQ=255) tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:498819/69‑1 (MQ=255) tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:641653/69‑1 (MQ=255) tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:701253/69‑1 (MQ=255) tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:1992695/69‑1 (MQ=255) tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:2130975/69‑1 (MQ=255) tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:2639699/69‑1 (MQ=255) tcactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtcatc < 1:2795345/69‑1 (MQ=255) cactcacCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGtc < 1:3238180/65‑1 (MQ=255) cAGCGCGCGTTGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1383145/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTGAtt < 1:1899181/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:785079/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:841170/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1485373/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1637289/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1358230/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:583369/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1727464/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:2773708/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:36056/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:265121/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1917851/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:197724/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:3213891/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:2597424/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:2352352/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:235529/69‑1 (MQ=255) cAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:294465/69‑1 (MQ=255) aGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:628070/68‑1 (MQ=255) aaCAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:2045658/56‑1 (MQ=255) aaCAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1146516/56‑1 (MQ=255) aaCAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:2800397/56‑1 (MQ=255) aaCAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:2969020/56‑1 (MQ=255) aaCAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:2988694/56‑1 (MQ=255) aaCAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:1685123/56‑1 (MQ=255) aaCAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:3380855/56‑1 (MQ=255) aaCAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:3426286/56‑1 (MQ=255) aaCAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:610170/56‑1 (MQ=255) aaCAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCAtt < 1:378249/56‑1 (MQ=255) | ATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATCACTCACCCGCCAGCGCGCGTGGGAACAGTACATTGTTTTCCAGACTGATGTGATCCATCAGGTCATCAATCAGTTCATT > minE/2879326‑2879443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |