Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,879,777 G→A 30.3% Y83Y (TAC→TAT ytfE ← predicted regulator of cell morphogenesis and cell wall metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,879,7770GA30.3% 76.7 / 35.1 79Y83Y (TAC→TATytfEpredicted regulator of cell morphogenesis and cell wall metabolism
Reads supporting (aligned to +/- strand):  ref base G (24/31);  new base A (12/12);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.68e-01

TGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCG  >  minE/2879712‑2879841
                                                                 |                                                                
tGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAg                                                               >  1:1157693/1‑69 (MQ=255)
tGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAg                                                               >  1:2261680/1‑69 (MQ=255)
tGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAg                                                               <  1:3129052/69‑1 (MQ=255)
      cgcTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACg                                                         <  1:3417588/69‑1 (MQ=255)
          cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                     <  1:2456059/69‑1 (MQ=255)
          cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                     <  1:250593/69‑1 (MQ=255)
          cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                     <  1:703820/69‑1 (MQ=255)
          cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                     <  1:2563164/69‑1 (MQ=255)
          cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                     <  1:1340547/69‑1 (MQ=255)
          cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                     <  1:2922079/69‑1 (MQ=255)
          cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                     <  1:1706051/69‑1 (MQ=255)
                tAGTCGCTTGCAGAATCAGCTCCGGGAGTTGCTCGCGGTGACGATCGTGGt                                                                 <  1:3035146/51‑1 (MQ=255)
                tAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                                                                 <  1:2239784/51‑1 (MQ=255)
                tAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                                                                 <  1:2008808/51‑1 (MQ=255)
                tAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                                                                 <  1:1961428/51‑1 (MQ=255)
                tAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                                                                 <  1:1567975/51‑1 (MQ=255)
                tAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                                                                 <  1:432664/51‑1 (MQ=255)
                tAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                                                                 <  1:523905/51‑1 (MQ=255)
                tAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                                                                 <  1:61440/51‑1 (MQ=255)
                tAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                                                                 <  1:746077/51‑1 (MQ=255)
                tAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                                                                 <  1:1238211/51‑1 (MQ=255)
                tAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGt                                                                 <  1:74656/51‑1 (MQ=255)
                  gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc                                              <  1:1607015/68‑1 (MQ=255)
                  gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc                                              <  1:181041/68‑1 (MQ=255)
                  gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc                                              <  1:1204648/68‑1 (MQ=255)
                  gTCGCATGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc                                              <  1:1725413/68‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:3159589/69‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:575671/69‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:2673742/69‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:616340/69‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:2549177/69‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:1645400/69‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:1281608/69‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:1299579/69‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:1617320/69‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:1329618/69‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:1438679/69‑1 (MQ=255)
                     gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                          <  1:1497352/69‑1 (MQ=255)
                     gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                          >  1:2189597/1‑69 (MQ=255)
                     gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                          >  1:3035726/1‑69 (MQ=255)
                     gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                          >  1:3146866/1‑69 (MQ=255)
                     gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                          >  1:2950904/1‑69 (MQ=255)
                     gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                          >  1:2886438/1‑69 (MQ=255)
                     gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                          >  1:1324964/1‑69 (MQ=255)
                     gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                          >  1:875324/1‑69 (MQ=255)
                       ttGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatgat                                        <  1:3149656/69‑1 (MQ=255)
                               tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTTGTAg                                                               >  1:2883071/1‑38 (MQ=255)
                               tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGcc                                 >  1:1527682/1‑68 (MQ=255)
                               tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCa                                >  1:1199181/1‑69 (MQ=255)
                               tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCa                                >  1:826492/1‑69 (MQ=255)
                               tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCAATGATTTCTGCCa                                >  1:305662/1‑69 (MQ=255)
                                 aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                      <  1:631589/45‑1 (MQ=255)
                                 aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                      <  1:3092552/45‑1 (MQ=255)
                                 aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                      <  1:2089307/45‑1 (MQ=255)
                                 aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                      <  1:1043562/45‑1 (MQ=255)
                                 aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                      <  1:1903781/45‑1 (MQ=255)
                                  gCTCCTGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                      <  1:584639/44‑1 (MQ=255)
                                             tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc                    >  1:1721792/1‑67 (MQ=255)
                                             tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc                    >  1:2548437/1‑67 (MQ=255)
                                             tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc                    >  1:3073388/1‑67 (MQ=255)
                                             tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc                    >  1:444233/1‑67 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:1786053/1‑69 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:979180/1‑69 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:2381560/1‑69 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:733574/1‑69 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:1410127/1‑69 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:2678012/1‑69 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:3342438/1‑69 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:3308865/1‑69 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:291917/1‑69 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:1782076/1‑69 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:303441/1‑69 (MQ=255)
                                                 cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt              >  1:2108688/1‑69 (MQ=255)
                                                           aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGc                  >  1:2763736/1‑55 (MQ=255)
                                                           aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt    >  1:3395280/1‑69 (MQ=255)
                                                           aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt    >  1:199337/1‑69 (MQ=255)
                                                           aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt    >  1:2032793/1‑69 (MQ=255)
                                                           aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt    >  1:3081606/1‑69 (MQ=255)
                                                             cGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCg  >  1:200412/1‑69 (MQ=255)
                                                                 |                                                                
TGAACGCGCTCGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCG  >  minE/2879712‑2879841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: