Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,879,789 G→A 48.0% I79I (ATC→ATT ytfE ← predicted regulator of cell morphogenesis and cell wall metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,879,7890GA48.0% ‑0.3 / 41.5 50I79I (ATC→ATTytfEpredicted regulator of cell morphogenesis and cell wall metabolism
Reads supporting (aligned to +/- strand):  ref base G (21/5);  new base A (12/12);  total (33/17)
Fisher's exact test for biased strand distribution p-value = 3.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGC  >  minE/2879722‑2879855
                                                                   |                                                                  
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                                   <  1:2563164/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                                   <  1:250593/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                                   <  1:2456059/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                                   <  1:2922079/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                                   <  1:1340547/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                                   <  1:703820/69‑1 (MQ=255)
cGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatga                                                                   <  1:1706051/69‑1 (MQ=255)
        gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc                                                            <  1:1204648/68‑1 (MQ=255)
        gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc                                                            <  1:181041/68‑1 (MQ=255)
        gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc                                                            <  1:1607015/68‑1 (MQ=255)
        gTCGCATGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc                                                            <  1:1725413/68‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:1497352/69‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:1617320/69‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:1645400/69‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:1438679/69‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:2549177/69‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:2673742/69‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:616340/69‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:575671/69‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:1329618/69‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:3159589/69‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:1281608/69‑1 (MQ=255)
           gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg                                                        <  1:1299579/69‑1 (MQ=255)
           gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                                        >  1:875324/1‑69 (MQ=255)
           gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                                        >  1:2886438/1‑69 (MQ=255)
           gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                                        >  1:2189597/1‑69 (MQ=255)
           gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                                        >  1:1324964/1‑69 (MQ=255)
           gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                                        >  1:2950904/1‑69 (MQ=255)
           gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                                        >  1:3035726/1‑69 (MQ=255)
           gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg                                                        >  1:3146866/1‑69 (MQ=255)
             ttGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatgat                                                      <  1:3149656/69‑1 (MQ=255)
                     tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGcc                                               >  1:1527682/1‑68 (MQ=255)
                     tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCa                                              >  1:1199181/1‑69 (MQ=255)
                     tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCa                                              >  1:826492/1‑69 (MQ=255)
                     tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCAATGATTTCTGCCa                                              >  1:305662/1‑69 (MQ=255)
                       aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                                    <  1:1043562/45‑1 (MQ=255)
                       aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                                    <  1:1903781/45‑1 (MQ=255)
                       aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                                    <  1:2089307/45‑1 (MQ=255)
                       aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                                    <  1:631589/45‑1 (MQ=255)
                       aGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                                    <  1:3092552/45‑1 (MQ=255)
                        gCTCCTGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACgatg                                                                    <  1:584639/44‑1 (MQ=255)
                                   tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc                                  >  1:2548437/1‑67 (MQ=255)
                                   tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc                                  >  1:1721792/1‑67 (MQ=255)
                                   tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc                                  >  1:444233/1‑67 (MQ=255)
                                   tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc                                  >  1:3073388/1‑67 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:3342438/1‑69 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:979180/1‑69 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:1786053/1‑69 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:1782076/1‑69 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:733574/1‑69 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:1410127/1‑69 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:3308865/1‑69 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:303441/1‑69 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:2108688/1‑69 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:291917/1‑69 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:2381560/1‑69 (MQ=255)
                                       cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt                            >  1:2678012/1‑69 (MQ=255)
                                                 aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGc                                >  1:2763736/1‑55 (MQ=255)
                                                 aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt                  >  1:3395280/1‑69 (MQ=255)
                                                 aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt                  >  1:199337/1‑69 (MQ=255)
                                                 aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt                  >  1:3081606/1‑69 (MQ=255)
                                                 aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt                  >  1:2032793/1‑69 (MQ=255)
                                                   cGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCg                >  1:200412/1‑69 (MQ=255)
                                                                 atgatATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGc  >  1:739419/1‑69 (MQ=255)
                                                                   |                                                                  
CGACTTTAGTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGC  >  minE/2879722‑2879855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: