| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,879,792 | A→G | 47.1% | H78H (CAT→CAC) | ytfE ← | predicted regulator of cell morphogenesis and cell wall metabolism |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,879,792 | 0 | A | G | 47.1% | ‑0.5 / 42.5 | 51 | H78H (CAT→CAC) | ytfE | predicted regulator of cell morphogenesis and cell wall metabolism |
| Reads supporting (aligned to +/- strand): ref base A (22/5); new base G (12/12); total (34/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGC > minE/2879730‑2879860 | gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc < 1:1204648/68‑1 (MQ=255)gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc < 1:181041/68‑1 (MQ=255)gTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc < 1:1607015/68‑1 (MQ=255)gTCGCATGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTc < 1:1725413/68‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1645400/69‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:2549177/69‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:2673742/69‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:3159589/69‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:575671/69‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:616340/69‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1617320/69‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1497352/69‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1438679/69‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1329618/69‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1299579/69‑1 (MQ=255) gCTTGCAGGATCAGCTCCGGCAGCTGCTCGCGATGGCGATCGTGATAGCGCACAATAATGTGGTCgatg < 1:1281608/69‑1 (MQ=255) gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:1324964/1‑69 (MQ=255) gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:3146866/1‑69 (MQ=255) gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:3035726/1‑69 (MQ=255) gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:2950904/1‑69 (MQ=255) gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:2886438/1‑69 (MQ=255) gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:2189597/1‑69 (MQ=255) gCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatg > 1:875324/1‑69 (MQ=255) ttGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCgatgat < 1:3149656/69‑1 (MQ=255) tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGcc > 1:1527682/1‑68 (MQ=255) tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCa > 1:1199181/1‑69 (MQ=255) tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCa > 1:826492/1‑69 (MQ=255) tCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCAATGATTTCTGCCa > 1:305662/1‑69 (MQ=255) tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc > 1:1721792/1‑67 (MQ=255) tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc > 1:444233/1‑67 (MQ=255) tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc > 1:3073388/1‑67 (MQ=255) tGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTAc > 1:2548437/1‑67 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:1786053/1‑69 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:979180/1‑69 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:1410127/1‑69 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:733574/1‑69 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:3342438/1‑69 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:3308865/1‑69 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:1782076/1‑69 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:303441/1‑69 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:291917/1‑69 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:2108688/1‑69 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:2678012/1‑69 (MQ=255) cgcgATGGCGATCGTGATAGCGCACAATAATGTGGTCGATGATTTCCGCCAGCGGGGCGCTACGCCAGt > 1:2381560/1‑69 (MQ=255) aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGc > 1:2763736/1‑55 (MQ=255) aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt > 1:199337/1‑69 (MQ=255) aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt > 1:3081606/1‑69 (MQ=255) aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt > 1:2032793/1‑69 (MQ=255) aTCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAAt > 1:3395280/1‑69 (MQ=255) cGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCg > 1:200412/1‑69 (MQ=255) atgatATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGc > 1:739419/1‑69 (MQ=255) atGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTg < 1:3300432/68‑1 (MQ=255) atGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTg < 1:1664307/68‑1 (MQ=255) atGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGc < 1:1134217/69‑1 (MQ=255) | GTCGCTTGCAGAATCAGCTCCGGCAGTTGCTCGCGGTGACGATCGTGGTAGCGCACGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGC > minE/2879730‑2879860 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |