| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,883,501 | C→A | 52.7% | L369L (CTG→CTT) | cpdB ← | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,883,501 | 0 | C | A | 52.7% | ‑4.1 / 136.8 | 112 | L369L (CTG→CTT) | cpdB | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base C (8/45); new base A (19/40); total (27/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 112 total aligned reads displayed.
AATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGA > minE/2883435‑2883556 | aaTGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAg > 1:572843/1‑69 (MQ=255) aTGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAg < 1:1724597/69‑1 (MQ=255) cTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATa > 1:385675/1‑69 (MQ=255) cTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATa > 1:3384064/1‑69 (MQ=255) cTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATa > 1:2396804/1‑69 (MQ=255) cTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGAACGGTCGGATCGTCCTGCACCAGCGCCAGATa > 1:1500595/1‑69 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:1582413/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:3407920/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:2366693/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:3307793/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:2401365/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:1916633/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:864710/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:2512256/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:2988592/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:297009/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:637251/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:86651/68‑1 (MQ=255) cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACata < 1:1291518/68‑1 (MQ=255) tttttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATAtt < 1:341617/69‑1 (MQ=255) tttttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATAtt < 1:2480401/69‑1 (MQ=255) tttttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATAtt < 1:1630886/69‑1 (MQ=255) tttttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATAtt < 1:294253/69‑1 (MQ=255) tttttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATAtt < 1:194880/69‑1 (MQ=255) tttttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATAtt < 1:701113/69‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:2907661/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:2197239/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:2269955/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:525115/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:1950174/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:1271665/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:2578568/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:1801021/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:1758777/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:1433617/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:1577309/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:349143/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:1510134/67‑1 (MQ=255) tGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCAAAAGCGCCAGATAGCTATACATATTGt < 1:813728/67‑1 (MQ=255) cgTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAg < 1:2682735/69‑1 (MQ=255) cgTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAg < 1:2043697/69‑1 (MQ=255) cgTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAg < 1:2315767/69‑1 (MQ=255) cgTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAg < 1:2084691/69‑1 (MQ=255) cgTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAg < 1:1398618/69‑1 (MQ=255) cgTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAg < 1:587817/69‑1 (MQ=255) cgTTGTTCAACACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAg < 1:1541524/69‑1 (MQ=255) tgttCACCACCTGCACGGTCTGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGAtt < 1:1078820/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCTGCAGAtt < 1:3079015/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCTGCAGAtt < 1:2747207/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCTGCAGAtt < 1:2607217/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCTGCAGAtt < 1:2761173/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCTGCAGAtt < 1:3200192/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCTGCAGAtt < 1:827126/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCTGCAGAtt < 1:1959779/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCTGCAGAtt < 1:1103696/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCTGCAGAtt < 1:780197/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGAtt < 1:364972/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGAtt < 1:2955229/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGAtt < 1:2564125/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGAtt < 1:1463315/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGAtt < 1:1607928/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGAtt < 1:559512/69‑1 (MQ=255) tgttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGAtt < 1:2141908/69‑1 (MQ=255) gttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCTGCAGAtt < 1:3087695/68‑1 (MQ=255) gttCACCACCTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCTGCAGAtt < 1:1918473/68‑1 (MQ=255) caccacCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTAcc > 1:3326812/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCtata > 1:1404703/1‑46 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTGCCGa > 1:1902842/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCg > 1:1050034/1‑68 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:2610771/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:3097981/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:700731/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:1015440/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:2385037/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:2096504/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:200915/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:851025/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:1572698/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:1447775/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:1268044/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:1161926/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGa > 1:493176/1‑69 (MQ=255) ccaccTGCACGGTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTCTCGGCAGATTTACCGa > 1:707495/1‑69 (MQ=255) accTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGAtt > 1:2474799/1‑69 (MQ=255) cACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCtt > 1:2010400/1‑69 (MQ=255) ggTCGGATCGTCCTGCACAAGCGCCAGATAGCTATACATATTGt < 1:1964111/44‑1 (MQ=255) ggATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACg < 1:379210/69‑1 (MQ=255) ggATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACg < 1:3294186/69‑1 (MQ=255) ggATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACg < 1:2873634/69‑1 (MQ=255) ggATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACg < 1:2414561/69‑1 (MQ=255) ggATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACg < 1:1924026/69‑1 (MQ=255) ggATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACg < 1:1823418/69‑1 (MQ=255) ggATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGCCTTGCTGACg < 1:2612837/69‑1 (MQ=255) aTCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGa < 1:3415753/68‑1 (MQ=255) aTCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGa < 1:2556512/68‑1 (MQ=255) aTCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGa < 1:1992588/68‑1 (MQ=255) aTCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGa < 1:857246/68‑1 (MQ=255) aTCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGa < 1:1640878/68‑1 (MQ=255) aTCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGa < 1:1295663/68‑1 (MQ=255) aTCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGa < 1:937819/68‑1 (MQ=255) | AATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGA > minE/2883435‑2883556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |