| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,883,780 | G→C | 44.4% | A276A (GCC→GCG) | cpdB ← | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,883,780 | 0 | G | C | 44.4% | 25.6 / 108.2 | 98 | A276A (GCC→GCG) | cpdB | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (29/25); new base C (18/25); total (47/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01 | |||||||||||
TCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAA > minE/2883712‑2883845 | tCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTg > 1:1242986/1‑69 (MQ=255) gACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGC‑‑‑AACACCATTCAGCGTGCCTTTCGCGat < 1:769936/69‑1 (MQ=255) ccACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGa < 1:517115/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCATCGTGCCTTTCGCGat < 1:1463334/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:3054027/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:2133602/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:2206601/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:1995557/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:220970/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:2435849/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:254889/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:1854163/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:1784738/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:2825195/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:2831777/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:1632918/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:324418/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:1080357/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:859920/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:3393535/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:401376/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:822316/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:1181907/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:1375657/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:558372/69‑1 (MQ=255) cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTAGCGat < 1:1654543/69‑1 (MQ=255) acacCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:2660721/68‑1 (MQ=255) aaGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGc > 1:997127/1‑69 (MQ=255) atgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGcccc > 1:3323515/1‑69 (MQ=255) tgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGcccc > 1:2670282/1‑68 (MQ=255) tgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGccc > 1:1733074/1‑67 (MQ=255) tgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCt > 1:258671/1‑69 (MQ=255) tgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCt > 1:2607113/1‑69 (MQ=255) tgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCt > 1:2908358/1‑69 (MQ=255) tgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCt > 1:882010/1‑69 (MQ=255) tgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCt > 1:1070120/1‑69 (MQ=255) tgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCt > 1:1055227/1‑69 (MQ=255) cGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGa < 1:349433/69‑1 (MQ=255) cGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTCGCGat < 1:2439703/55‑1 (MQ=255) aCCGCCGGAACCCCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCt > 1:558596/1‑69 (MQ=255) aCCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTAcc > 1:1804620/1‑68 (MQ=255) aCCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCt > 1:1137798/1‑69 (MQ=255) aCCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCt > 1:859409/1‑69 (MQ=255) aCCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCt > 1:134980/1‑69 (MQ=255) aCCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCt > 1:2046911/1‑69 (MQ=255) aCCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCt > 1:291546/1‑69 (MQ=255) aCCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCt > 1:1718287/1‑69 (MQ=255) aCCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCt > 1:2537694/1‑69 (MQ=255) cgGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGaa < 1:1651613/69‑1 (MQ=255) cgGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGaa < 1:532302/69‑1 (MQ=255) cgGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGaa < 1:446160/69‑1 (MQ=255) cgGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGaa < 1:1606029/69‑1 (MQ=255) gtaaCACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGaa < 1:2576179/66‑1 (MQ=255) aaCACCATTCAGCGTGCCTTTCGCGATATCAGCCCCTTCGATATCAGCa > 1:2191282/1‑49 (MQ=255) aaCACCATTCAGCGTGCCTTTCGCGATATCAGCCCCTTCGATATCAGCa > 1:1634485/1‑49 (MQ=255) ccATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACgg > 1:605340/1‑67 (MQ=255) ccATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGc > 1:1586087/1‑68 (MQ=255) ccATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGc > 1:534239/1‑68 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:2093/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:2235484/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:43522/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:2138689/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:3057327/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:2942451/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:2280244/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:2855504/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:168473/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:2822894/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:1157703/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:2381717/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:2057378/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:2418405/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:1840824/1‑35 (MQ=255) ccATTCAGCGTGCCTTTCGCGATATCAGCCCCTTc > 1:1126062/1‑35 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:1175786/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:729301/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:2136548/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:1985105/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:880382/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:1371729/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:512133/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:3290384/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:1683710/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:2425632/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:1776425/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:2761597/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:1477548/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:2973439/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:1982811/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:224232/69‑1 (MQ=255) tCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACAGCGTGAg < 1:850029/69‑1 (MQ=255) gTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAg < 1:2062023/64‑1 (MQ=255) ccTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCa > 1:865430/1‑69 (MQ=255) ccTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCa > 1:2564200/1‑69 (MQ=255) ccTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCa > 1:1946432/1‑69 (MQ=255) ttGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCaaa > 1:1607892/1‑68 (MQ=255) ttGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCaaa > 1:2725568/1‑68 (MQ=255) ttGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCaaa > 1:224211/1‑68 (MQ=255) ttGGCGATATAAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGa > 1:3138071/1‑46 (MQ=255) | TCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAA > minE/2883712‑2883845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |