| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,884,215 | T→C | 25.6% | A131A (GCA→GCG) | cpdB ← | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,884,215 | 0 | T | C | 25.6% | 131.4 / 43.2 | 113 | A131A (GCA→GCG) | cpdB | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base T (39/45); new base C (16/13); total (55/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 114 total aligned reads displayed.
AACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTA > minE/2884153‑2884281 | aaCATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAg > 1:3430864/1‑68 (MQ=255)aaCATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAg > 1:2697997/1‑68 (MQ=255)aaCATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAg > 1:2270418/1‑68 (MQ=255)aaCATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAg > 1:143650/1‑68 (MQ=255)aaCATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAg > 1:1210456/1‑68 (MQ=255) tGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTc < 1:3002649/69‑1 (MQ=255) aaTGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTa > 1:691740/1‑69 (MQ=255) aCGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTa < 1:2699372/69‑1 (MQ=255) aCGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTa < 1:1449549/69‑1 (MQ=255) aCGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTa < 1:2355411/69‑1 (MQ=255) aCGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTa < 1:1583995/69‑1 (MQ=255) aCGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTa < 1:579496/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCTCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:2248782/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:889106/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:2030050/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:3100550/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:3403236/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:1874056/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:45748/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:1741061/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:1581303/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:2453136/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:2482035/69‑1 (MQ=255) tGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:1327225/68‑1 (MQ=255) ggCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGt > 1:2158928/1‑59 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGt > 1:2923329/1‑41 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGt > 1:807538/1‑41 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGt > 1:319060/1‑41 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2818508/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2804854/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2923648/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2734686/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2634381/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2157527/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2560452/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:651350/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:687345/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:1481373/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:713073/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:1415743/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:1388624/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:130886/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:804895/1‑69 (MQ=255) aGGGAATTTCGCTCCTGCCAGCGCATTTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCaa > 1:2503444/1‑69 (MQ=255) aGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTa < 1:520419/52‑1 (MQ=255) aGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTg > 1:2086780/1‑60 (MQ=255) ggAATTTCGCTCCTGCCAGCTCA‑TTTTTCAGGTAATCCAGACCGTAGTTa < 1:790752/50‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:2628293/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt > 1:2611849/1‑69 (MQ=255) gAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:3079099/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:3418441/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:813984/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:1021171/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:1671954/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:1937481/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:1956058/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGAGGTTGCCAAGCGt < 1:757372/69‑1 (MQ=255) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACtt > 1:2077093/1‑51 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACt > 1:186667/1‑51 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:2865100/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:1034355/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:850205/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:1260814/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:1305044/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:671686/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:548395/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:523283/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:385552/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:377486/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:1905284/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCA‑TTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:2492044/1‑52 (MQ=39) ttCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCg > 1:3261968/1‑69 (MQ=255) ttCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCg > 1:3374398/1‑69 (MQ=255) ttCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCg > 1:309382/1‑69 (MQ=255) ttCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCg > 1:2669869/1‑69 (MQ=255) ttCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCg > 1:2030957/1‑69 (MQ=255) tCCTGCGAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:3070933/68‑1 (MQ=255) tCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:2556839/68‑1 (MQ=255) tCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:814191/68‑1 (MQ=255) tCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:1046551/68‑1 (MQ=255) tCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:2225422/68‑1 (MQ=255) tCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:1347055/68‑1 (MQ=255) tCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:1619617/68‑1 (MQ=255) tCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:2429053/68‑1 (MQ=255) tCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:1894224/68‑1 (MQ=255) tCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:1820988/68‑1 (MQ=255) tCCTGACAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:161665/68‑1 (MQ=255) ccTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:1459735/69‑1 (MQ=255) ccTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:2345146/69‑1 (MQ=255) ccTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:2906317/69‑1 (MQ=255) ccTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:2623682/69‑1 (MQ=255) ccTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:2205241/69‑1 (MQ=255) ccTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:308268/69‑1 (MQ=255) ccTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:438519/69‑1 (MQ=255) ccTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:1576202/69‑1 (MQ=255) ccTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:2412847/69‑1 (MQ=255) ccTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:1836710/69‑1 (MQ=255) ccTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:3132839/69‑1 (MQ=255) cTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:673243/68‑1 (MQ=255) tGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:497550/65‑1 (MQ=255) | AACATTGGCTGTTTGGTTCTGGCGTCAATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCA‑TTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTA > minE/2884153‑2884281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |