| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,884,233 | G→A | 30.7% | Y125Y (TAC→TAT) | cpdB ← | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,884,233 | 0 | G | A | 30.7% | 156.0 / 77.4 | 159 | Y125Y (TAC→TAT) | cpdB | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (31/79); new base A (36/13); total (67/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
Only 100 of 161 total aligned reads displayed.
AATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTATTTAA > minE/2884179‑2884298 | aaTGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTa > 1:691740/1‑69 (MQ=255) aCGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTa < 1:780964/69‑1 (MQ=255) aCGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTa < 1:1449549/69‑1 (MQ=255) aCGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTa < 1:579496/69‑1 (MQ=255) aCGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTa < 1:2699372/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:3100550/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:3403236/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:1581303/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:45748/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:889106/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:1741061/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:2030050/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:2453136/69‑1 (MQ=255) ttGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:2181456/69‑1 (MQ=255) tGGCATTTACATAAGGGAATTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAAc < 1:1327225/68‑1 (MQ=255) aTTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTg < 1:320888/69‑1 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGt > 1:807538/1‑41 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2634381/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2560452/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2923648/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2157527/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:2427251/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:1388624/1‑69 (MQ=255) tACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGtt > 1:130886/1‑69 (MQ=255) aGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTa < 1:520419/52‑1 (MQ=255) aGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTg > 1:2086780/1‑60 (MQ=255) ggAATTTCGCTCCTGCCAGCTCATTTTTCAGGTAATCCAGACCGTAGTTa < 1:790752/50‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:2786271/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:813984/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:1937481/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt < 1:1021171/69‑1 (MQ=255) gAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGt > 1:930097/1‑69 (MQ=255) aTTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAACtt > 1:2077093/1‑51 (MQ=39) aTTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAACt > 1:186667/1‑51 (MQ=39) aTTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:2492044/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:850205/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:671686/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:2865100/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:548395/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:1260814/1‑52 (MQ=39) aTTTCGCTCCCGCCAGCGCATTTTTCAGATAATCCAGACCGTAGTTAAACTc > 1:1945293/1‑52 (MQ=39) ttCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCg > 1:3374398/1‑69 (MQ=255) ttCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCg > 1:3261968/1‑69 (MQ=255) tCCTGCGAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:3070933/68‑1 (MQ=255) tCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:2225422/68‑1 (MQ=255) tCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:1046551/68‑1 (MQ=255) tCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:1619617/68‑1 (MQ=255) tCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:1894224/68‑1 (MQ=255) tCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACgg < 1:1820988/68‑1 (MQ=255) ccTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:308268/69‑1 (MQ=255) ccTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:438519/69‑1 (MQ=255) ccTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:3132839/69‑1 (MQ=255) ccTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:2906317/69‑1 (MQ=255) ccTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:1836710/69‑1 (MQ=255) ccTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:2205241/69‑1 (MQ=255) ccTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:2345146/69‑1 (MQ=255) ccTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:2623682/69‑1 (MQ=255) cTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGta < 1:673243/68‑1 (MQ=255) aGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTc < 1:1088022/68‑1 (MQ=255) aGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTc < 1:1423080/68‑1 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:383091/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:3199874/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:2429329/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:2385798/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:3422874/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:376176/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:967300/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:2376299/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:468874/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:508727/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:1480102/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:300464/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:705014/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:178701/1‑37 (MQ=255) cATTTTTCAGATAATCCAGACCGTAGTTAAACTCGTg > 1:2573814/1‑37 (MQ=255) tttCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGt < 1:2099669/66‑1 (MQ=255) tttCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGt < 1:63567/66‑1 (MQ=255) tttCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGt < 1:1404704/66‑1 (MQ=255) tttCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGt < 1:3074983/66‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:1343914/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:748034/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:1121677/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:2324358/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:1610136/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:1574674/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:1580471/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:1662550/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:2207097/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:3031889/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:1718485/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:2499703/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:281164/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:2765449/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:2617379/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:2679108/68‑1 (MQ=255) tCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTAttt < 1:1887423/68‑1 (MQ=255) aGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTATTTaa < 1:2504781/68‑1 (MQ=255) aGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTATTTaa < 1:1486421/68‑1 (MQ=255) aGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTATTTaa < 1:1280637/68‑1 (MQ=255) aGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTATTTaa < 1:1033516/68‑1 (MQ=255) | AATGACGTTGGCATTTACATAAGGGAATTTCGCTCCTGCCAGCGCATTTTTCAGGTAATCCAGACCGTAGTTAAACTCGTGGTTGCCAAGCGTTCCGACGGTATAGTCCAGCGTATTTAA > minE/2884179‑2884298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |