| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,884,356 | G→T | 15.3% | A84A (GCC→GCA) | cpdB ← | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,884,356 | 0 | G | T | 15.3% | 168.8 / 16.6 | 78 | A84A (GCC→GCA) | cpdB | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (43/23); new base T (10/2); total (53/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 | |||||||||||
TAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCACTTCATTGCGGG > minE/2884296‑2884423 | tAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCg < 1:2685373/68‑1 (MQ=255) gCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTc < 1:2112700/69‑1 (MQ=255) cTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTccc > 1:1176027/1‑69 (MQ=255) cTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTccc > 1:2243807/1‑69 (MQ=255) taGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGa > 1:847711/1‑69 (MQ=255) taGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGa > 1:1384846/1‑69 (MQ=255) taGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGa > 1:841724/1‑69 (MQ=255) gAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGc > 1:2159266/1‑69 (MQ=255) gAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGc > 1:2301570/1‑69 (MQ=255) gAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGc > 1:3024391/1‑69 (MQ=255) gAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGc > 1:3042548/1‑69 (MQ=255) gAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGc > 1:3236401/1‑69 (MQ=255) gAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGc > 1:1643628/1‑69 (MQ=255) gAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGc > 1:1403544/1‑69 (MQ=255) tatCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGcc > 1:355273/1‑67 (MQ=255) tatCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGt > 1:98400/1‑69 (MQ=255) tatCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGt > 1:2130345/1‑69 (MQ=255) tCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTcc < 1:2442246/49‑1 (MQ=255) tCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGtt > 1:2915674/1‑68 (MQ=255) tCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGtt > 1:61532/1‑68 (MQ=255) tCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGtt > 1:2334072/1‑68 (MQ=255) tCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGtt > 1:232790/1‑68 (MQ=255) tCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGtt > 1:1119224/1‑68 (MQ=255) tCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGtt > 1:987538/1‑68 (MQ=255) tCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGtt > 1:1354241/1‑68 (MQ=255) tCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGtt > 1:1964757/1‑68 (MQ=255) tCACCTGCTTTTAATCCTTACGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGtt > 1:2263049/1‑68 (MQ=255) aCCTGCTTTTAATCCTTTCGCCGACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATc < 1:961699/69‑1 (MQ=255) aCCTGCTTTTAATCCTTTCGCCGACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATc < 1:1501248/69‑1 (MQ=255) aCCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTAt > 1:2230597/1‑68 (MQ=255) aCCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATc > 1:1287190/1‑69 (MQ=255) aCCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATc > 1:1422257/1‑69 (MQ=255) aCCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATc > 1:543909/1‑69 (MQ=255) aCCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATc > 1:3151958/1‑69 (MQ=255) aCCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATc > 1:2987382/1‑69 (MQ=255) aCCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATc > 1:1957822/1‑69 (MQ=255) aCCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATc > 1:2645874/1‑69 (MQ=255) aCCTACTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATc > 1:544760/1‑69 (MQ=255) tttAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAg < 1:581265/68‑1 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCATATCGCCGTTATCAACCAGTACGCTg > 1:2975078/1‑69 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACTCTg < 1:2022916/69‑1 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg < 1:1659723/69‑1 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg < 1:3125188/69‑1 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg < 1:3217750/69‑1 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg < 1:341824/69‑1 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg < 1:2824107/69‑1 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg > 1:494917/1‑69 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg < 1:770607/69‑1 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg < 1:2598102/69‑1 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg < 1:2594164/69‑1 (MQ=255) ccTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg < 1:1315335/69‑1 (MQ=255) cTTTCGCCGAGATGTAATCGGCCAGCTGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg < 1:1649224/68‑1 (MQ=255) tCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTg < 1:2679470/65‑1 (MQ=255) gCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGttttt < 1:761907/68‑1 (MQ=255) gCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGttttt < 1:850291/68‑1 (MQ=255) gCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGttttt < 1:2138751/68‑1 (MQ=255) gCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGttttt < 1:2554882/68‑1 (MQ=255) gCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGttttt < 1:2628010/68‑1 (MQ=255) gCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGttttt < 1:195005/68‑1 (MQ=255) gCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGttttt < 1:2661350/68‑1 (MQ=255) gACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCGCCCAGTACGc > 1:663483/1‑58 (MQ=255) gACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCGACCAGTACg > 1:1689073/1‑57 (MQ=255) gACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCGACCAGTACGc > 1:1976656/1‑58 (MQ=255) gACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCGACCAGTACGc > 1:2276994/1‑58 (MQ=255) gACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCGACCAGTACGc > 1:2283024/1‑58 (MQ=255) gACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCGACCAGTACGc > 1:2092194/1‑58 (MQ=255) gACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCGACCAGTACGc > 1:2562450/1‑58 (MQ=255) gACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCGACCAGTACGc > 1:379908/1‑58 (MQ=255) gACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCGACCAGTACGc > 1:2395914/1‑58 (MQ=255) gACATGTAATCTGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCGACCAGTACGc > 1:3172558/1‑58 (MQ=255) tAATCGGCCAGCGGACTGCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCACTTCATTg > 1:1567059/1‑69 (MQ=255) tAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCACTTCATTg > 1:2317335/1‑69 (MQ=255) tAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCACTTCATTg > 1:2699592/1‑69 (MQ=255) aTCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCACTTCATTGCg > 1:965076/1‑69 (MQ=255) aTCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCACTTCATTGCg > 1:1960814/1‑69 (MQ=255) aTCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCACTTCATTGCg > 1:2406006/1‑69 (MQ=255) aTCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCACTTCATTGCg > 1:2685159/1‑69 (MQ=255) aTCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCACTTCATTGCg > 1:2404071/1‑69 (MQ=255) ggCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACg > 1:2991739/1‑46 (MQ=255) ggCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCACTTCATTGCggg > 1:276829/1‑68 (MQ=255) | TAATGCCTTATAGACCGGGTGAATATCACCTGCTTTTAATCCTTTCGCCGACATGTAATCGGCCAGCGGACTCCCCTGAATCAAATCGCCGTTATCAACCAGTACGCTGTTTTTCACTTCATTGCGGG > minE/2884296‑2884423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |