| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,891,504 | A→T | 100% | G53G (GGA→GGT) | ytfQ → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,891,504 | 0 | A | T | 75.4% | 96.3 / 30.1 | 65 | G53G (GGA→GGT) | ytfQ | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (16/0); new base T (5/44); total (21/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCG > minE/2891452‑2891558 | gCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTg > 1:3213716/1‑69 (MQ=255)gCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTg > 1:621017/1‑69 (MQ=255) gTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATg > 1:1724891/1‑69 (MQ=255) cAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGc > 1:1248674/1‑68 (MQ=255) cAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGc > 1:1884322/1‑68 (MQ=255) cAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGc > 1:456651/1‑68 (MQ=255) gtgGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGaa > 1:3289214/1‑69 (MQ=255) gtgGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGaa > 1:2390276/1‑69 (MQ=255) gtgGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGaa > 1:3435045/1‑69 (MQ=255) gtgGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGaa > 1:1504270/1‑69 (MQ=255) gtgGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGaa > 1:504831/1‑69 (MQ=255) gtgGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGaa > 1:782248/1‑69 (MQ=255) gCGAAAAGTTAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:2195850/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:910995/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:958827/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:2467610/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:2473843/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:2676050/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:2699476/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:2967136/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:2992275/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:468991/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:3279807/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:2471643/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:3377014/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:478412/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:2334348/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:1965886/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:1226193/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:2202611/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:1458693/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:1936952/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:2157920/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:162653/61‑1 (MQ=255) gCGAAAAGTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGc < 1:1663606/61‑1 (MQ=255) gAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGcc > 1:1587260/1‑43 (MQ=255) gAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGcc > 1:187528/1‑43 (MQ=255) gAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGcc > 1:2412806/1‑43 (MQ=255) gAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGc > 1:3039579/1‑42 (MQ=255) gTGAAGCCGAAAAACGCGGTATCACAGTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGAt > 1:3117506/1‑67 (MQ=38) gTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGAt > 1:3363825/1‑67 (MQ=38) gTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGAt > 1:2332527/1‑67 (MQ=38) gTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGAt > 1:1514019/1‑67 (MQ=38) gTGAAGCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGAt > 1:1992872/1‑67 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:3159808/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:94419/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:795336/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:1253982/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:2467662/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:1504902/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:1078506/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:251947/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:2145176/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:3381915/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:2066138/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:2801294/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:3356358/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:3289287/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:3237195/69‑1 (MQ=38) gCCGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:3118654/69‑1 (MQ=38) ccGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:1718567/68‑1 (MQ=38) ccGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:2832111/68‑1 (MQ=38) ccGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:2461641/68‑1 (MQ=38) ccGAAAAACGCGGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:1153241/68‑1 (MQ=38) cgGTATCACACTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCg < 1:1486236/58‑1 (MQ=38) | GCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCG > minE/2891452‑2891558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |