| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 249,667 | C→A | 37.7% | A71A (GCC→GCA) | proY → | predicted cryptic proline transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 249,667 | 0 | C | A | 37.7% | 43.5 / 40.0 | 53 | A71A (GCC→GCA) | proY | predicted cryptic proline transporter |
| Reads supporting (aligned to +/- strand): ref base C (19/14); new base A (11/9); total (30/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
TTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGG > minE/249600‑249733 | ttatCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgcc > 1:198385/1‑68 (MQ=255)ttatCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgcc > 1:1881622/1‑68 (MQ=255) atCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCTgccgc > 1:1075535/1‑68 (MQ=255) atCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgccgcc > 1:2810986/1‑69 (MQ=255) atCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgccgcc > 1:227746/1‑69 (MQ=255) atCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgccgcc > 1:2159362/1‑69 (MQ=255) aTCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTtc > 1:885318/1‑69 (MQ=255) gcgTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGt < 1:153145/69‑1 (MQ=255) tatCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATg > 1:1657285/1‑68 (MQ=255) tCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATgcgc > 1:2185282/1‑69 (MQ=255) tgcgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATgcgc > 1:1025047/1‑63 (MQ=255) tgcgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATgcgc > 1:1000671/1‑63 (MQ=255) tgcgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATgcgc > 1:1182171/1‑63 (MQ=255) cgtgcgCTGGGGGATATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:3048534/69‑1 (MQ=255) cgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:3175740/69‑1 (MQ=255) cgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:2200015/69‑1 (MQ=255) cgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:1211424/69‑1 (MQ=255) cgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:2455172/69‑1 (MQ=255) cgtgcgCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAAc < 1:1337439/69‑1 (MQ=255) tCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGt > 1:3132930/1‑69 (MQ=255) tCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGt > 1:2277902/1‑69 (MQ=255) tCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGt > 1:4828/1‑69 (MQ=255) tCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTAGGCCCGCTGGCAGGt > 1:1079337/1‑69 (MQ=255) ggTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGGAGGAAAAc < 1:377336/49‑1 (MQ=255) ggTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTa < 1:375142/69‑1 (MQ=255) ggTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTa < 1:3129976/69‑1 (MQ=255) gTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTa < 1:608713/68‑1 (MQ=255) cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTTGCAGGTTACAtt > 1:3147312/1‑69 (MQ=255) cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:3147564/1‑69 (MQ=255) cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:3048662/1‑69 (MQ=255) cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:2132694/1‑69 (MQ=255) cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:2090214/1‑69 (MQ=255) cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:413541/1‑69 (MQ=255) cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:1980048/1‑69 (MQ=255) cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:1800412/1‑69 (MQ=255) cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:1763325/1‑69 (MQ=255) cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt > 1:1739943/1‑69 (MQ=255) cATAACCCGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTAGGCCCGCTGGCAGGTTACAtt > 1:2665960/1‑69 (MQ=255) atcaaCCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt < 1:448888/66‑1 (MQ=255) atcaaCCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt < 1:2653142/66‑1 (MQ=255) atcaaCCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAtt < 1:526038/66‑1 (MQ=255) tAACCCGGCCTCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:2681408/1‑69 (MQ=255) tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCgg > 1:2170786/1‑48 (MQ=255) tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAt > 1:1607091/1‑66 (MQ=255) tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:383064/1‑69 (MQ=255) tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:2638407/1‑69 (MQ=255) tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:1060625/1‑69 (MQ=255) tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:2290000/1‑69 (MQ=255) tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:2112862/1‑69 (MQ=255) tAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTAc > 1:1441672/1‑69 (MQ=255) ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGTCCCGCTGGCAGGTTACATTACCGGc < 1:289958/69‑1 (MQ=255) ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:2914348/69‑1 (MQ=255) ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:2761734/69‑1 (MQ=255) ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:1467317/69‑1 (MQ=255) ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:2216204/69‑1 (MQ=255) ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:1240917/69‑1 (MQ=255) ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:1926456/69‑1 (MQ=255) ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:1343827/69‑1 (MQ=255) ccGGCAGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGc < 1:1642640/69‑1 (MQ=255) gccgccAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTgg < 1:647320/69‑1 (MQ=255) gccgccAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTgg < 1:1528157/69‑1 (MQ=255) | TTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGG > minE/249600‑249733 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |