| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,891,687 | T→C | 31.7% | I114I (ATT→ATC) | ytfQ → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,891,687 | 0 | T | C | 31.7% | 127.1 / 78.5 | 149 | I114I (ATT→ATC) | ytfQ | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (60/41); new base C (31/16); total (92/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 161 total aligned reads displayed.
AAAAGAGGCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACATCCTCGAAGGCAAGTTGAT > minE/2891636‑2891755 | aaaaGAGGCGAAAGATGCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAAtc > 1:1011213/1‑69 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAAtc > 1:2157915/1‑69 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAAtc > 1:1451515/1‑69 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAAtc > 1:278980/1‑69 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAAtc > 1:3182900/1‑69 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAAtc > 1:404360/1‑69 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAAt > 1:1312880/1‑68 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAAt > 1:1916312/1‑68 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAAt > 1:1232104/1‑68 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAAt > 1:640047/1‑68 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTATTCTTTCTCGATCGTTCCATTGATGTGAAAGACAAAtc > 1:577194/1‑69 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCATTGATGTGAAAGACAAAtc > 1:394000/1‑69 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCATTGATGTGAAAGACAAAtc > 1:734304/1‑69 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCATTGATGTGAAAGACAAAtc > 1:2058382/1‑69 (MQ=255)aaaaGAGGCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCATTGATGTGAAAGACAAAtc > 1:1267536/1‑69 (MQ=255)aaaaGAGGCGAAAGATGACGAAATCCCGGTATTCTTGCTCGATCGTTCCATAGATGTGAAAGACAAAt > 1:2915436/1‑68 (MQ=255) gagGCGAAAGATGCCGAAATCCCGTTATTCTTGCTCGATCGTTCCATTGATGTGAAAGACAAAtctctc < 1:636958/69‑1 (MQ=255) gagGCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCATTGATGTGAAAGACAAAtctctc < 1:2478787/69‑1 (MQ=255) ggCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCAtt < 1:1898687/46‑1 (MQ=255) ggCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCAtt < 1:1795354/46‑1 (MQ=255) ggCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCAtt < 1:2304811/46‑1 (MQ=255) ggCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCAtt < 1:543908/46‑1 (MQ=255) ggCGAAAGATGACGAAATCCCGGTATTCTTGCTCGATCGTTCCAtt < 1:3197667/46‑1 (MQ=255) gCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCAtt < 1:1565902/45‑1 (MQ=255) tGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGaccac > 1:1434670/1‑69 (MQ=255) gCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAATCTCTCTATATGaccacc < 1:2467738/69‑1 (MQ=255) gCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAATCTCTCTATATGaccacc < 1:2209042/69‑1 (MQ=255) gCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAATCTCTCTATATGaccacc < 1:884555/69‑1 (MQ=255) gCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAATCTCTCTATATGaccacc < 1:460890/69‑1 (MQ=255) gCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAATCTCTCTATATGaccacc < 1:1892750/69‑1 (MQ=255) gCCGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAATCTCTCTATATGaccacc < 1:1796171/69‑1 (MQ=255) gCCGAAATCCCGGTATTCTTGCTCGATCGTTCCATTGATGTGAGAGACAAATCTCTCTATATGaccacc > 1:2144617/1‑69 (MQ=255) ccGAAATCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAATCTCTCTATATGaccacc < 1:1007700/68‑1 (MQ=255) gAAATCCCGGTATTCTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGt < 1:14747/68‑1 (MQ=255) gAAATCCCGGTATTCTTGCCCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:1109030/69‑1 (MQ=255) aaaTCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAATCTCTCTATATGACCACCGtta > 1:301449/1‑67 (MQ=255) aaaTCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAATCTCTCTATATGACCACCGtta > 1:1063370/1‑67 (MQ=255) aaaTCCCGGTCTTCTTGCTTGACCGTTCCATCGATGTGAAAGACAAATCTCTCTATATGACCACCGtta > 1:2193802/1‑67 (MQ=255) gTATTCTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGt < 1:2229149/59‑1 (MQ=255) ttCTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGacaac > 1:80789/1‑69 (MQ=255) ttCTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGacaac > 1:3126726/1‑69 (MQ=255) ttCTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGacaac > 1:2292818/1‑69 (MQ=255) ttCTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGacaac > 1:3125140/1‑69 (MQ=255) cTTGCTCTATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:2587609/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAAtctctc > 1:2758008/1‑40 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:2118818/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:422150/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:135363/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:473807/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:501516/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:121427/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:365997/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:1082434/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:1529729/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:61552/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc < 1:2365352/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCa > 1:2997748/1‑56 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGacaaca > 1:2425988/1‑68 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGacaaca > 1:2595487/1‑68 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGacaaca > 1:1940302/1‑68 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:3412012/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:3252916/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:1861163/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:3207361/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:512736/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:1088101/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:566272/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:621220/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:1032570/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:811947/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:857306/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:1997068/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:2828904/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:1630022/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACAt > 1:2008276/1‑69 (MQ=255) cTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCCCTATATGACCACCGTc < 1:3084824/55‑1 (MQ=255) cTTGCTCGATCGTTCCATTGATATGAAAGACAAATCTCTCTATATGACCACCGTc < 1:109484/55‑1 (MQ=255) gaccGTTCCATCGATGTGAAAGACAAATCTCTCTATATGACCACCGTTACTGCCGACAACATCCTCGa > 1:1263351/4‑68 (MQ=255) gaccGTTCCATCGATGTGAAAGACAAATCTCTCTATATGACCACCGTTACTGCCGACAACATCCTCGa > 1:3275371/4‑68 (MQ=255) gaccGTTCCATCGATGTGAAAGACAAATCTCTCTATATGACCACCGTTACTGCCGACAACATCCTCGa > 1:2263113/4‑68 (MQ=255) gaccGTTCCATCGATGTGAAAGACAAATCTCTCTATATGACCACCGTTACTGCCGACAACATCCTCGa > 1:237410/4‑68 (MQ=255) gaccGTTCCATCGATGTGAAAGACAAATCTCTCTATATGACCACCGTTACTGCCGACAACATCCTCGa > 1:2575588/4‑68 (MQ=255) gaccGTTCCATCGATGTGAAAGACAAATCTCTCTATATGACCACCGTTACTGCCGACAACATACt > 1:1673815/4‑65 (MQ=255) gTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACATCCTCGAAGGCa < 1:1948592/69‑1 (MQ=255) gTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACATCCTCGAAGGCa < 1:2979397/69‑1 (MQ=255) gTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACATCCTCGAAGGCa < 1:1684803/69‑1 (MQ=255) gTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGGCACTGCCGACAACATCCTCGAAGGCa < 1:1408679/69‑1 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc > 1:867261/1‑41 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc > 1:2144183/1‑41 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc > 1:123965/1‑41 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc > 1:704262/1‑41 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc > 1:1053344/1‑41 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc > 1:571222/1‑41 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTc > 1:2655424/1‑41 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACATCCTCGAAGGCAAg > 1:663990/1‑68 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACATCCTCGAAGGCAAg > 1:2286558/1‑68 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACATCCTCGAAGGCAAg > 1:795244/1‑68 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACATCCTCGAAGGCAAg > 1:2040595/1‑68 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACATCCTCGAAGGCAAg > 1:2544933/1‑68 (MQ=255) ccATTGATGTGAAAGACAAATCTCTCTAAATGACCACCGTCACTGCCGACAACATCCTCGAAGGCAAg > 1:3030830/1‑68 (MQ=255) | AAAAGAGGCGAAAGATGCCGAAATCCCGGTATTCTTGCTCGATCGTTCCATTGATGTGAAAGACAAATCTCTCTATATGACCACCGTCACTGCCGACAACATCCTCGAAGGCAAGTTGAT > minE/2891636‑2891755 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |