| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,891,872 | A→G | 19.4% | K176R (AAG→AGG) | ytfQ → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,891,872 | 0 | A | G | 19.4% | 67.9 / 8.5 | 46 | K176R (AAG→AGG) | ytfQ | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (8/29); new base G (4/5); total (12/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01 | |||||||||||
GCTGCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTAAAGGCAAAGAA > minE/2891807‑2891939 | gCTGCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAg < 1:310118/67‑1 (MQ=255)gCTGCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAg < 1:2288733/67‑1 (MQ=255)gCTGCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAg < 1:3189204/67‑1 (MQ=255)gCTGCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAg < 1:3159443/67‑1 (MQ=255)gCTGCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAg < 1:62665/67‑1 (MQ=255)gCTGCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAg < 1:1853095/67‑1 (MQ=255)gCTGCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAg < 1:752756/67‑1 (MQ=255)gCTGCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAg < 1:1462046/67‑1 (MQ=255)gCTGCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAg < 1:2852955/67‑1 (MQ=255) gCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAGAATgc > 1:2642348/1‑69 (MQ=255) gCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAGAATgc > 1:3047135/1‑69 (MQ=255) gCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAGAATgc > 1:56376/1‑69 (MQ=255) cAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAg < 1:2394248/46‑1 (MQ=255) aTTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAGGAATGCGCCAAATATCAAAATTATCCGCTCGCa > 1:1323833/1‑68 (MQ=255) aTTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAGGAATGCGCCAAATATCAAAATTATCCGCTCGCa > 1:1770097/1‑68 (MQ=255) aTTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAGGAATGCGCCAAATATCAAAATTATCCGCTCGCa > 1:2349398/1‑68 (MQ=255) aTTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAGGAATGCGCCAAATATCAAAATTATCCGATCGc > 1:2898350/1‑67 (MQ=255) gACCGTAAGAAAGGCTTTGCCGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCa > 1:3396185/1‑69 (MQ=255) gACCGTAAGAAAGGCTTTGCCGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCa > 1:1465997/1‑69 (MQ=255) gACCGTAAGAAAGGCTTTGCCGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCa > 1:2450515/1‑69 (MQ=255) gACCGTAAGAAAGGCTTTGCCGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCa > 1:2432093/1‑69 (MQ=255) gACCGTAAGAAAGGCTTTGCCGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCa > 1:2337347/1‑69 (MQ=255) cGTAAGAAAGGCTTTGCCGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGt < 1:2616307/69‑1 (MQ=255) tAAGAAAGGCTTTGCCGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGa < 1:3398385/69‑1 (MQ=255) aGGCTTTGCCGAAGCCATTAGGAATGCGCCAAATATCAAAATTATCCGCTCGCAGTCAGGTGACTTCAc < 1:577298/69‑1 (MQ=255) aGGCTTTGCCGAAGCCATTAGGAATGCGCCAAATATCAAAATTATCCGCTCGCAGTCAGGTGACTTCAc < 1:1113854/69‑1 (MQ=255) aGGCTTTGCCGAAGCCATTAGGAATGCGCCAAATATCAAAATTATCCGCTCGCAGTCAGGTGACTTCAc < 1:798402/69‑1 (MQ=255) aGGCTTTGCCGAAGCCATTAGGAATGCGCCAAATATCAAAATTATCCGCTCGCAGTCAGGTGACTTCAc < 1:685410/69‑1 (MQ=255) aGGCTTTGCCGAAGCCATTAGGAATGCGCCAAATATCAAAATTATCCGCTCGCAGTCAGGTGACTTCAc < 1:573285/69‑1 (MQ=255) cGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTa < 1:2298986/68‑1 (MQ=255) cGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTa < 1:1165089/68‑1 (MQ=255) cGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTa < 1:845200/68‑1 (MQ=255) cGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTa < 1:160421/68‑1 (MQ=255) cGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTa < 1:1636678/68‑1 (MQ=255) cGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTa < 1:1875025/68‑1 (MQ=255) cGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTa < 1:3185207/68‑1 (MQ=255) cGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTAAGGTGACTTCACCCGCAGTa < 1:2653808/68‑1 (MQ=255) gAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTa < 1:923442/67‑1 (MQ=255) tAAGAATGCGGCAAATATCAAAATCATCCGCTCGCAGTCAGGTg < 1:2060538/44‑1 (MQ=255) tAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTg < 1:3310053/44‑1 (MQ=255) tAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTg < 1:412719/44‑1 (MQ=255) tAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTg < 1:2380919/44‑1 (MQ=255) tAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTg < 1:2260485/44‑1 (MQ=255) tAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTg < 1:2167538/44‑1 (MQ=255) tAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTg < 1:2028731/44‑1 (MQ=255) tAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTg < 1:1441728/44‑1 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGt < 1:515854/42‑1 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGt < 1:481001/42‑1 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGt < 1:1070011/42‑1 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGt < 1:923519/42‑1 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGt < 1:2586266/42‑1 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGt < 1:226106/42‑1 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGt < 1:1879387/42‑1 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGt < 1:180724/42‑1 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTc > 1:878378/1‑48 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTAAAGGCAaagaa > 1:3298294/1‑69 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTAAAGGCAaagaa > 1:3155294/1‑69 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTAAAGGCAaagaa > 1:1473188/1‑69 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTAAAGGCAaagaa > 1:1290446/1‑69 (MQ=255) aaGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTAAAGGCAaagaa > 1:111336/1‑69 (MQ=255) aaGAATGCGCCAAATATCAAAATCAACCGCTCGCAGTCAGGt < 1:1222836/42‑1 (MQ=255) aGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGt < 1:3067879/41‑1 (MQ=255) aGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGt < 1:1264527/41‑1 (MQ=255) | GCTGCAGGGCACCGTTGGGGCCAGCGTCGCCATTGACCGTAAGAAAGGCTTTGCCGAAGCCATTAAGAATGCGCCAAATATCAAAATCATCCGCTCGCAGTCAGGTGACTTCACCCGCAGTAAAGGCAAAGAA > minE/2891807‑2891939 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |