| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,892,465 | G→A | 100% | E8E (GAG→GAA) | ytfR → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,892,465 | 0 | G | A | 76.3% | 21.0 / 3.0 | 21 | E8E (GAG→GAA) | ytfR | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (3/2); new base A (16/0); total (19/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAG > minE/2892400‑2892533 | ttATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGAtc > 1:82051/1‑69 (MQ=255)ttATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGAtc > 1:1974304/1‑69 (MQ=255)ttATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGAtc > 1:2018938/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:2160651/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:821112/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:592220/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:562232/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:483421/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:2744526/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:26278/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:1274913/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:2139807/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:2107902/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:1902238/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:1595044/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAgg > 1:1456110/1‑69 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAg > 1:2449475/1‑68 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAAATCCTCCGCACCGAAg > 1:2061313/1‑68 (MQ=255) gggAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACAAGGAAATCCTCCGCACCGAAgg > 1:1804722/1‑69 (MQ=255) cGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGc < 1:163711/54‑1 (MQ=255) gaccAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGAc < 1:3029756/69‑1 (MQ=255) gaTCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAg > 1:416125/1‑69 (MQ=255) gaTCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAg > 1:652260/1‑69 (MQ=255) | TTATCATGCCTACTGGGAGCACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAG > minE/2892400‑2892533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |