| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,893,110 | C→T | 44.6% | F223F (TTC→TTT) | ytfR → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,893,110 | 0 | C | T | 44.6% | 7.8 / 34.8 | 47 | F223F (TTC→TTT) | ytfR | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (13/13); new base T (13/8); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTCACTTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTGGGGCGCGAG > minE/2893045‑2893176 | actcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTa < 1:376324/69‑1 (MQ=255)actcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTa < 1:1922987/69‑1 (MQ=255)actcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTa < 1:166480/69‑1 (MQ=255)actcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTa < 1:2923562/69‑1 (MQ=255)actcactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTa < 1:1742488/69‑1 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:2732714/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:2773975/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:2310932/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:2845051/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:1993044/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:1972853/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:349614/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:1530345/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:1195488/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:751722/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:86668/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTTGtgg > 1:1001139/1‑67 (MQ=255) ctcattTTCTCGATCAGGTTTATCAGGTTAGCGATCGGATCACCGACTTACGCAACGGCAGTTTTGtgg > 1:1714537/1‑67 (MQ=255) cactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGc > 1:2778240/1‑69 (MQ=255) cactTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGc > 1:2335667/1‑69 (MQ=255) cTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGa > 1:1979627/1‑69 (MQ=255) aGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTAc > 1:2861353/1‑69 (MQ=255) aGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTAc > 1:2676507/1‑69 (MQ=255) cAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTAc > 1:1079963/1‑65 (MQ=255) aTCGGATCACCGTCTTACGCAACGGCAGTTTCGTa < 1:1031387/35‑1 (MQ=255) aTCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCg < 1:2179249/69‑1 (MQ=255) aTCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCg < 1:860921/69‑1 (MQ=255) aTCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCg < 1:796994/69‑1 (MQ=255) aTCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCg < 1:2921406/69‑1 (MQ=255) aTCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCg < 1:3125423/69‑1 (MQ=255) tCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGa < 1:731656/69‑1 (MQ=255) gCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAatga < 1:926606/66‑1 (MQ=255) aaCGGCAGTTTTGTGGGCTGTCGGGAAACCCGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTg < 1:3176372/69‑1 (MQ=255) aaCGGCAGTTTTGTGGGCTGTCGGGAAACCCGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTg < 1:3369380/69‑1 (MQ=255) aaCGGCAGTTTTGTGGGCTGTCGGGAAACCCGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTg < 1:398087/69‑1 (MQ=255) aaCGGCAGTTTTGTGGGCTGTCGGGAAACCCGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTg < 1:54191/69‑1 (MQ=255) aaCGGCAGTTTTGTGGGCTGTCGGGAAACCCGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTg < 1:2890954/69‑1 (MQ=255) aaCGGCAGTTTTGTGGGCTGTCGGGAAACCCGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTg < 1:11366/69‑1 (MQ=255) aaCGGCAGTTTTGTGGGCTGTCGGGAAACCCGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTg < 1:2618018/69‑1 (MQ=255) gggaGTTTTGTGGGCTGTCGGGAAACCCGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTg < 1:1248312/63‑1 (MQ=255) ttCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAat > 1:3381862/1‑53 (MQ=255) ttCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAat > 1:1822144/1‑53 (MQ=255) ttCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAat > 1:507739/1‑53 (MQ=255) ttCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAat > 1:2045014/1‑53 (MQ=255) ttCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAat > 1:216620/1‑53 (MQ=255) ttCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTGGGGCGCGAg > 1:1833120/1‑69 (MQ=255) ttCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTGGGGCGCGAg > 1:1137044/1‑69 (MQ=255) | ACTCACTTTCTCGATCAGGTCTATCAGGTCAGCGATCGGATCACCGTCTTACGCAACGGCAGTTTCGTAGGCTGTCGGGAAACGTGCGAGCTACCGCAGATCGAACTGGTAAAAATGATGCTGGGGCGCGAG > minE/2893045‑2893176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |