Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,894,374 T→C 27.6% T139T (ACT→ACC ytfT → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,894,3740TC27.6% 106.5 / 42.5 101T139T (ACT→ACCytfTpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (58/15);  new base C (18/10);  total (76/25)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 102 total aligned reads displayed.

ATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAG  >  minE/2894309‑2894428
                                                                 |                                                      
aTCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTg                                                     <  1:703348/69‑1 (MQ=255)
aTCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTg                                                     <  1:2968300/69‑1 (MQ=255)
aTCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTg                                                     <  1:1429151/69‑1 (MQ=255)
aTCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTg                                                     <  1:1307858/69‑1 (MQ=255)
 tCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGa                                                    <  1:15934/69‑1 (MQ=255)
 tCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGa                                                    <  1:1343142/69‑1 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGAt                                                   >  1:2809845/1‑68 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATc                                                  >  1:2559543/1‑69 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATc                                                  >  1:1940954/1‑69 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATc                                                  >  1:194477/1‑69 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATc                                                  >  1:2029030/1‑69 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATc                                                  >  1:2408367/1‑69 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATc                                                  >  1:2458476/1‑69 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATc                                                  >  1:3114796/1‑69 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATc                                                  >  1:3352451/1‑69 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATc                                                  >  1:136762/1‑69 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATc                                                  >  1:1475892/1‑69 (MQ=255)
   cTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATc                                                  >  1:431644/1‑69 (MQ=255)
         ggATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATg                                            >  1:2875218/1‑69 (MQ=255)
         ggATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATg                                            >  1:2128915/1‑69 (MQ=255)
         ggATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATg                                            >  1:2329349/1‑69 (MQ=255)
            ttGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCt                                                >  1:1603198/1‑62 (MQ=255)
            ttGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCt                                                >  1:2330483/1‑62 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCTCTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:2424220/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCActc                                                       >  1:540216/1‑40 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGAt                                                   >  1:2130669/1‑44 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCg                                     >  1:3120514/1‑58 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGt                           >  1:2447125/1‑68 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGt                           >  1:3060448/1‑68 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGt                           >  1:2123693/1‑68 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGt                           >  1:2774728/1‑68 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGt                           >  1:1214485/1‑68 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGt                           >  1:1668888/1‑68 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGt                           >  1:1687417/1‑68 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGt                           >  1:1240646/1‑68 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:380010/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:2623753/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:2727893/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:1594615/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:303006/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:1287098/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:3198611/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:3290562/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:1272466/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:3432840/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:1946625/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:387464/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:398663/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:559856/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:630188/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:838378/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:861477/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:896834/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:94844/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:968353/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:1937182/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:1927826/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:2155195/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:1657542/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:1756819/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:174364/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          >  1:1836168/1‑69 (MQ=255)
                           cTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          <  1:1692743/69‑1 (MQ=255)
                           cTGGTAGCGATCCTCAAAAATCAGCCGTTTGGCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTg                          <  1:2849567/69‑1 (MQ=255)
                             ggTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgc                        <  1:2308287/69‑1 (MQ=255)
                             ggTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgc                        <  1:2283651/69‑1 (MQ=255)
                             ggTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgc                        <  1:1806638/69‑1 (MQ=255)
                             ggTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgc                        <  1:1828641/69‑1 (MQ=255)
                             ggTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgc                        <  1:3004787/69‑1 (MQ=255)
                             ggTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgc                        <  1:2986035/69‑1 (MQ=255)
                             ggTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgc                        <  1:1018467/69‑1 (MQ=255)
                             ggTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgc                        <  1:1592565/69‑1 (MQ=255)
                             ggTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgc                        <  1:1592744/69‑1 (MQ=255)
                             ggTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgc                        <  1:2096880/69‑1 (MQ=255)
                               tAGCGATCCTCAAAATTCAGGCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:586660/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:674464/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:1225410/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:733930/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:891057/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:1953186/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:3331019/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:231212/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:2698390/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:2699487/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:2717224/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:2535877/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:2958188/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:1432041/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:2316474/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:358542/1‑69 (MQ=255)
                               tAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATACTGATGGTCGCCGGGCGCGGCGTGgcg                       >  1:2997697/1‑68 (MQ=255)
                               aaGCGATCCTCAAAATTCAGCCGTTTGTCGCCACCCTGATCCTGATGGTCGCCGGGCGCGGCGTGgcgc                      >  1:684512/2‑69 (MQ=255)
                                       cTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATc              <  1:713233/69‑1 (MQ=255)
                                       cTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATc              <  1:2072822/69‑1 (MQ=255)
                                       cTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATc              <  1:1305199/69‑1 (MQ=255)
                                              ttCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCAccgccg       >  1:3270711/1‑69 (MQ=255)
                                                   ccGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAg  <  1:1503812/69‑1 (MQ=255)
                                                   ccGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAg  <  1:2780882/69‑1 (MQ=255)
                                                    cGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCAccgccg       >  1:192652/1‑63 (MQ=255)
                                                       ttGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGt                           <  1:251339/40‑1 (MQ=255)
                                                                 |                                                      
ATCCTGGCGGGATTGTGGAACGGCATACTGGTAGCGATCCTCAAAATTCAGCCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAG  >  minE/2894309‑2894428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: