| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,894,425 | G→A | 46.1% | G156G (GGG→GGA) | ytfT → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,894,425 | 0 | G | A | 46.1% | ‑0.4 / 17.8 | 26 | G156G (GGG→GGA) | ytfT | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (2/12); new base A (7/5); total (9/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01 | |||||||||||
CCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTG > minE/2894360‑2894488 | ccGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAg < 1:1503812/69‑1 (MQ=255)ccGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAg < 1:2780882/69‑1 (MQ=255) ccTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCTCCGGACAGATCGTCACATTTAACTcac < 1:3282244/68‑3 (MQ=255) ccTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCTCCGGACAGATCGTCACATTTAACTcac < 1:1565261/68‑3 (MQ=255) ccTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCTCCGGACAGATCGTCACATTTAACTcac < 1:3224136/68‑3 (MQ=255) ccTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCTCCGGACAGATCGTCACATTTAACTcac < 1:1658803/68‑3 (MQ=255) ccTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCTCCGGACAGATCGTCACATTTAACTcac < 1:2597372/68‑3 (MQ=255) ggTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGAtct > 1:1579130/1‑69 (MQ=255) cGCCGGGCGCGGCGTGGCTCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGAtctct < 1:158830/68‑1 (MQ=255) cGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCTCCGGAtctct < 1:2702812/68‑1 (MQ=255) cGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGAtctct < 1:1348470/68‑1 (MQ=255) cGCCGGGCGCGGCGTGGCGCAACTGATCACCGCC‑GGCAGATCGTCACGTTTAACTCGCCGGATCTCTCa > 1:1908810/1‑69 (MQ=255) tGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCtgt < 1:1908066/69‑1 (MQ=255) tGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCtgt < 1:3164314/69‑1 (MQ=255) gATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCtgt < 1:1602801/68‑1 (MQ=255) cACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTgttgtt < 1:1751430/69‑1 (MQ=255) cACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTgttgtt < 1:696339/69‑1 (MQ=255) cACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGAGCTCTCATGGTTCGTCAGTGGATCGCTgttgtt < 1:2221609/69‑1 (MQ=255) aCCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTgttgtt < 1:3237072/68‑1 (MQ=255) ccgGACTGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg > 1:2382455/1‑68 (MQ=255) ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCt > 1:1687407/1‑58 (MQ=255) ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg > 1:1725604/1‑68 (MQ=255) ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg > 1:1714721/1‑68 (MQ=255) ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg > 1:1550964/1‑68 (MQ=255) ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg > 1:364185/1‑68 (MQ=255) ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg > 1:646098/1‑68 (MQ=255) | CCGTTTGTCGCCACTCTGATCCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTG > minE/2894360‑2894488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |