Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,894,437 G→A 39.9% T160T (ACG→ACA)  ytfT → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,894,4370GA39.9% 10.3 / 17.1 30T160T (ACG→ACA) ytfTpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (5/13);  new base A (7/5);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 1.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

CCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGT  >  minE/2894380‑2894499
                                                         |                                                              
ccTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCTCCGGACAGATCGTCACATTTAACTcac                                                      <  1:1565261/68‑3 (MQ=255)
ccTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCTCCGGACAGATCGTCACATTTAACTcac                                                      <  1:3282244/68‑3 (MQ=255)
ccTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCTCCGGACAGATCGTCACATTTAACTcac                                                      <  1:1658803/68‑3 (MQ=255)
ccTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCTCCGGACAGATCGTCACATTTAACTcac                                                      <  1:3224136/68‑3 (MQ=255)
ccTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCTCCGGACAGATCGTCACATTTAACTcac                                                      <  1:2597372/68‑3 (MQ=255)
      ggTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGAtct                                               >  1:1579130/1‑69 (MQ=255)
         cGCCGGGCGCGGCGTGGCTCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGAtctct                                             <  1:158830/68‑1 (MQ=255)
         cGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCTCCGGAtctct                                             <  1:2702812/68‑1 (MQ=255)
         cGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGAtctct                                             <  1:1348470/68‑1 (MQ=255)
         cGCCGGGCGCGGCGTGGCGCAACTGATCACCGCC‑GGCAGATCGTCACGTTTAACTCGCCGGATCTCTCa                                           >  1:1908810/1‑69 (MQ=255)
                                tGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCtgt                     <  1:3164314/69‑1 (MQ=255)
                                tGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCtgt                     <  1:1908066/69‑1 (MQ=255)
                                 gATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCtgt                     <  1:1602801/68‑1 (MQ=255)
                                    cACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTgttgtt                 <  1:696339/69‑1 (MQ=255)
                                    cACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTgttgtt                 <  1:1751430/69‑1 (MQ=255)
                                    cACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGAGCTCTCATGGTTCGTCAGTGGATCGCTgttgtt                 <  1:2221609/69‑1 (MQ=255)
                                     aCCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTgttgtt                 <  1:3237072/68‑1 (MQ=255)
                                         ccgGACTGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg             >  1:2382455/1‑68 (MQ=255)
                                         ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCt                       >  1:1687407/1‑58 (MQ=255)
                                         ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg             >  1:1725604/1‑68 (MQ=255)
                                         ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg             >  1:1714721/1‑68 (MQ=255)
                                         ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg             >  1:364185/1‑68 (MQ=255)
                                         ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg             >  1:646098/1‑68 (MQ=255)
                                         ccgGACAGATCGTCACATTTAACTCACCGGATCTCTCCTGGTTCGGCAGTGGATCGCTGTTGTTCCTg             >  1:1550964/1‑68 (MQ=255)
                                                  tCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCg    >  1:1714715/1‑68 (MQ=255)
                                                  tCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCg    >  1:3243588/1‑68 (MQ=255)
                                                  tCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCg    >  1:3400271/1‑68 (MQ=255)
                                                   cGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGt  <  1:1649183/69‑1 (MQ=255)
                                                   cGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGt  <  1:337450/69‑1 (MQ=255)
                                                     tCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGt  <  1:1908336/67‑1 (MQ=255)
                                                         |                                                              
CCTGATGGTCGCCGGGCGCGGCGTGGCGCAACTGATCACCGCCGGGCAGATCGTCACGTTTAACTCGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGT  >  minE/2894380‑2894499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑