Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,894,506 C→T 17.7% I183I (ATC→ATT ytfT → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,894,5060CT17.7% 134.6 / 16.6 84I183I (ATC→ATTytfTpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (23/46);  new base T (5/10);  total (28/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGAA  >  minE/2894445‑2894572
                                                             |                                                                  
cGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTg                                                              >  1:2500665/1‑68 (MQ=255)
cGCCGGATCTCTCATGATTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTg                                                              >  1:2455617/1‑68 (MQ=255)
cGCCGGATCACTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTg                                                              >  1:1306351/1‑68 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:421600/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:1929905/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:2352518/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:1754086/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:2538234/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:2585263/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:1533991/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:150598/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:2839519/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:1264803/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:1142994/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:3309950/67‑1 (MQ=255)
   cGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCt                                                            <  1:1053777/67‑1 (MQ=255)
             aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:280644/65‑1 (MQ=255)
             aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:2661138/65‑1 (MQ=255)
             aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:3049394/65‑1 (MQ=255)
             aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:1626581/65‑1 (MQ=255)
             aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:1665884/65‑1 (MQ=255)
             aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:38058/65‑1 (MQ=255)
             aTGGTTCGGCAGTGGATCGCTGTTGATCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:2251222/65‑1 (MQ=255)
                 ttCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:3292064/61‑1 (MQ=255)
                   cGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:2360602/59‑1 (MQ=255)
                           gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTTTTGa                                  <  1:3105829/69‑1 (MQ=255)
                           gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:1943789/69‑1 (MQ=255)
                           gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:2644570/69‑1 (MQ=255)
                           gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:2189293/69‑1 (MQ=255)
                           gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:1004222/69‑1 (MQ=255)
                           gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:1924993/69‑1 (MQ=255)
                           gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:1927566/69‑1 (MQ=255)
                                     gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGc                             >  1:2091284/1‑64 (MQ=255)
                                     gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:2135220/1‑69 (MQ=255)
                                     gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:3057169/1‑69 (MQ=255)
                                     gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:2394035/1‑69 (MQ=255)
                                     gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:1182389/1‑69 (MQ=255)
                                     gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:55289/1‑69 (MQ=255)
                                     gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:1791033/1‑69 (MQ=255)
                                     gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:2200752/1‑69 (MQ=255)
                                       tCCTGCCAACGCCAGTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCaaaa                         >  1:999054/1‑66 (MQ=255)
                                       tCCTGCCAACGCCAGTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCaaaa                         >  1:3264130/1‑66 (MQ=255)
                                       tCCTGCCAACGCCAGTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCaaaa                         >  1:973160/1‑66 (MQ=255)
                                       tCCTGCCAACGCCAGTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCaaaa                         >  1:2468155/1‑66 (MQ=255)
                                       tCCTGCCAACGCCAGTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCaaaa                         >  1:2535254/1‑66 (MQ=255)
                                                   cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:3173211/67‑1 (MQ=255)
                                                   cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:1042834/67‑1 (MQ=255)
                                                   cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:1305921/67‑1 (MQ=255)
                                                   cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:3048418/67‑1 (MQ=255)
                                                   cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:297418/67‑1 (MQ=255)
                                                   cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:648499/67‑1 (MQ=255)
                                                   cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:1437075/67‑1 (MQ=255)
                                                   cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:2393743/67‑1 (MQ=255)
                                                   cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:2413636/67‑1 (MQ=255)
                                                   cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:2559034/67‑1 (MQ=255)
                                                      tCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       <  1:2283910/69‑1 (MQ=255)
                                                      tCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       <  1:365593/69‑1 (MQ=255)
                                                      tCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       <  1:106812/69‑1 (MQ=255)
                                                      tCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       <  1:312308/69‑1 (MQ=255)
                                                       cATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGCCCCGCAAAACGGCGCTGGGGATGTTTa       >  1:216750/1‑68 (MQ=255)
                                                       cATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGttt        >  1:981461/1‑67 (MQ=255)
                                                       cATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       >  1:602019/1‑68 (MQ=255)
                                                       cATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       >  1:1598641/1‑68 (MQ=255)
                                                       cATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       >  1:946414/1‑68 (MQ=255)
                                                          tATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:257635/63‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGgcg                    >  1:3308291/1‑51 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:76234/1‑62 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:767865/1‑62 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:961444/62‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:2526805/62‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:933865/62‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:473196/62‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:3381945/62‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:3228444/1‑62 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:3206856/1‑62 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:2841999/62‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:2698200/1‑62 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:1403559/62‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:1018051/1‑62 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGaa  <  1:589697/69‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGaa  <  1:2204739/69‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGaa  <  1:188896/69‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGaa  <  1:1752371/69‑1 (MQ=255)
                                                           aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGaa  <  1:1491227/69‑1 (MQ=255)
                                                             |                                                                  
CGCCGGATCTCTCATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGAA  >  minE/2894445‑2894572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: