Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,894,522 A→C 26.6% I189L (ATC→CTC)  ytfT → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,894,5220AC26.6% 72.8 / 32.1 60I189L (ATC→CTC) ytfTpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (20/24);  new base C (5/11);  total (25/35)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGAA  >  minE/2894458‑2894572
                                                                |                                                  
aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:1665884/65‑1 (MQ=255)
aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:1626581/65‑1 (MQ=255)
aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:3049394/65‑1 (MQ=255)
aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:280644/65‑1 (MQ=255)
aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:38058/65‑1 (MQ=255)
aTGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:2661138/65‑1 (MQ=255)
aTGGTTCGGCAGTGGATCGCTGTTGATCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:2251222/65‑1 (MQ=255)
    ttCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:3292064/61‑1 (MQ=255)
      cGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTa                                                    <  1:2360602/59‑1 (MQ=255)
              gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTTTTGa                                  <  1:3105829/69‑1 (MQ=255)
              gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:2644570/69‑1 (MQ=255)
              gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:1004222/69‑1 (MQ=255)
              gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:1924993/69‑1 (MQ=255)
              gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:1927566/69‑1 (MQ=255)
              gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:1943789/69‑1 (MQ=255)
              gATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGa                                  <  1:2189293/69‑1 (MQ=255)
                        gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGc                             >  1:2091284/1‑64 (MQ=255)
                        gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:1182389/1‑69 (MQ=255)
                        gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:2394035/1‑69 (MQ=255)
                        gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:2200752/1‑69 (MQ=255)
                        gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:55289/1‑69 (MQ=255)
                        gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:2135220/1‑69 (MQ=255)
                        gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:1791033/1‑69 (MQ=255)
                        gttCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAAc                        >  1:3057169/1‑69 (MQ=255)
                          tCCTGCCAACGCCAGTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCaaaa                         >  1:2535254/1‑66 (MQ=255)
                          tCCTGCCAACGCCAGTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCaaaa                         >  1:2468155/1‑66 (MQ=255)
                          tCCTGCCAACGCCAGTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCaaaa                         >  1:3264130/1‑66 (MQ=255)
                          tCCTGCCAACGCCAGTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCaaaa                         >  1:999054/1‑66 (MQ=255)
                          tCCTGCCAACGCCAGTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCaaaa                         >  1:973160/1‑66 (MQ=255)
                                      cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:1042834/67‑1 (MQ=255)
                                      cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:1305921/67‑1 (MQ=255)
                                      cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:3048418/67‑1 (MQ=255)
                                      cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:297418/67‑1 (MQ=255)
                                      cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:2393743/67‑1 (MQ=255)
                                      cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:1437075/67‑1 (MQ=255)
                                      cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:3173211/67‑1 (MQ=255)
                                      cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:2559034/67‑1 (MQ=255)
                                      cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:648499/67‑1 (MQ=255)
                                      cagTCATTATTGCGGTGCTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:2413636/67‑1 (MQ=255)
                                         tCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       <  1:312308/69‑1 (MQ=255)
                                         tCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       <  1:365593/69‑1 (MQ=255)
                                         tCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       <  1:2283910/69‑1 (MQ=255)
                                         tCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       <  1:106812/69‑1 (MQ=255)
                                          cATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGCCCCGCAAAACGGCGCTGGGGATGTTTa       >  1:216750/1‑68 (MQ=255)
                                          cATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGttt        >  1:981461/1‑67 (MQ=255)
                                          cATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       >  1:1598641/1‑68 (MQ=255)
                                          cATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       >  1:946414/1‑68 (MQ=255)
                                          cATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTa       >  1:602019/1‑68 (MQ=255)
                                             tATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:257635/63‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGgcg                    >  1:3308291/1‑51 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:3206856/1‑62 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:1018051/1‑62 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:1403559/62‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:961444/62‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:933865/62‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:767865/1‑62 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:76234/1‑62 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:2526805/62‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:473196/62‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:2698200/1‑62 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:2841999/62‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         <  1:3381945/62‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGtt         >  1:3228444/1‑62 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGaa  <  1:589697/69‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGaa  <  1:2204739/69‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGaa  <  1:188896/69‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGaa  <  1:1752371/69‑1 (MQ=255)
                                              aTCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGaa  <  1:1491227/69‑1 (MQ=255)
                                                       cTGACGCTTCTCCTGTTCTGGCTGTTGACCCGCAAAACGGCACTGGGGATGt          <  1:607974/52‑1 (MQ=39)
                                                                |                                                  
ATGGTTCGGCAGTGGATCGCTGTTGTTCCTGCCAACGCCGGTCATTATCGCGGTGCTGACGCTTATCCTGTTCTGGCTGTTGACCCGCAAAACGGCGCTGGGGATGTTTATCGAA  >  minE/2894458‑2894572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: