Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,894,752 C→T 37.2% L265L (CTC→CTT ytfT → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,894,7520CT37.2% 33.1 / 34.8 59L265L (CTC→CTTytfTpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (2/35);  new base T (17/5);  total (19/40)
Fisher's exact test for biased strand distribution p-value = 1.27e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGG  >  minE/2894684‑2894818
                                                                    |                                                                  
gTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATtctc                                                                    >  1:2413083/1‑69 (MQ=255)
gTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATtctc                                                                    >  1:1869877/1‑69 (MQ=255)
gTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATtctc                                                                    >  1:1723702/1‑69 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGTggtggt                                                            <  1:437361/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCgttggt                                                            <  1:3001843/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:997555/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:2335640/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:2558162/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:2057490/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:198350/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:3306235/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:368106/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:581998/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:1572996/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:1231523/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:1081802/69‑1 (MQ=255)
        ggATATTCGCGGTGCCGATGCCAACAACGCAGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:1736058/69‑1 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAgtggtg                                                           >  1:1121707/1‑68 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:332549/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:3200264/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:794272/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:3137143/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:865822/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:2815803/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:1023118/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:977336/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:2352204/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:2471152/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:2253770/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:106766/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:1609118/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:1730366/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:1910130/1‑69 (MQ=255)
          atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:2093381/1‑69 (MQ=255)
                    tGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGcggcgg                                                <  1:957527/69‑1 (MQ=255)
                    tGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGcggcgg                                                <  1:150184/69‑1 (MQ=255)
                     gCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:2304190/56‑1 (MQ=255)
                     gCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:2649065/56‑1 (MQ=255)
                     gCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:956958/56‑1 (MQ=255)
                     gCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:656250/56‑1 (MQ=255)
                     gCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:1951427/56‑1 (MQ=255)
                         aTGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGGGATCGCt                                          <  1:2670426/69‑1 (MQ=255)
                          tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:1801296/69‑1 (MQ=255)
                          tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:1075581/69‑1 (MQ=255)
                          tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:772444/69‑1 (MQ=255)
                          tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:1696983/69‑1 (MQ=255)
                          tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:3291065/69‑1 (MQ=255)
                          tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:2122661/69‑1 (MQ=255)
                          tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:2093315/69‑1 (MQ=255)
                          tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:2782526/69‑1 (MQ=255)
                          tGCCAACAACGCTGCGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:3025831/69‑1 (MQ=255)
                                 aacGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATgggc                                   >  1:2537674/1‑69 (MQ=255)
                                 aacGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATgggc                                   >  1:1271174/1‑69 (MQ=255)
                                            aTGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTaa                        <  1:668065/69‑1 (MQ=255)
                                            aTGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTaa                        <  1:2920490/69‑1 (MQ=255)
                                                      ctggaCGCCATTCTTGCAGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCtt              <  1:302599/69‑1 (MQ=255)
                                                      ctggaCGCCATTCTTGCAGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCtt              <  1:3365155/69‑1 (MQ=255)
                                                      ctggaCGCCATTCTTGCAGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCtt              <  1:2020629/69‑1 (MQ=255)
                                                      ctggaCGCCATTCTTGCAGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCtt              <  1:2943426/69‑1 (MQ=255)
                                                       tggaCGCCATTCTTGCAGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCtt              <  1:1772031/68‑1 (MQ=255)
                                                           cGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCggt         <  1:3249630/69‑1 (MQ=255)
                                                           cGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCggt         <  1:1820347/69‑1 (MQ=255)
                                                                  ctcGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCttt                          >  1:524913/1‑45 (MQ=255)
                                                                  ctcGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCttt                          >  1:2644203/1‑45 (MQ=255)
                                                                  ctcGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCttt                          >  1:2369292/1‑45 (MQ=255)
                                                                  ctcGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGgtggtgtg   >  1:236154/1‑66 (MQ=255)
                                                                  ctcGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTgggg  >  1:487790/1‑69 (MQ=255)
                                                                    |                                                                  
GTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGG  >  minE/2894684‑2894818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: