| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 29,409 | C→G | 34.1% | S305S (TCC→TCG) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 29,409 | 0 | C | G | 34.1% | 39.1 / 32.1 | 44 | S305S (TCC→TCG) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base C (12/17); new base G (6/9); total (18/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAA > minE/29347‑29457 | cAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttct > 1:1034269/1‑69 (MQ=255)cAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttct > 1:2977399/1‑69 (MQ=255)cAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttc > 1:2358939/1‑68 (MQ=255) ttGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCgg < 1:834472/69‑1 (MQ=255) ttGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCgg < 1:1831634/69‑1 (MQ=255) gAACCCGAAATACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGc < 1:1572758/69‑1 (MQ=255) gAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGc < 1:1172551/69‑1 (MQ=255) gAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGc < 1:188589/69‑1 (MQ=255) gAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGc < 1:2887483/69‑1 (MQ=255) gAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAACCACGCGTGTCCCGTTCTTCGGCGCTGGc < 1:3023341/69‑1 (MQ=255) cGAAGAACGGTCGCCTGATTGTTATCGAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGa < 1:1076309/69‑1 (MQ=255) cGAAGAACGGTCGCCTGATTGTTATCGAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGa < 1:928579/69‑1 (MQ=255) cGAAGAACGGTCGCCTGATTGTTATCGAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGa < 1:561452/69‑1 (MQ=255) cGAAGAACGGTCGCCTGATTGTTATCGAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGa < 1:459435/69‑1 (MQ=255) cGAAGAACGGTCGCCTGATTGTTATCGAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGa < 1:2086857/69‑1 (MQ=255) cGAAGAACGGTCGCCTGATTGTTATCGAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGa < 1:2037735/69‑1 (MQ=255) gaagaaCGGTCGCCTGATTGTTATCGAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGa < 1:1515591/68‑1 (MQ=255) gtcTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGtt > 1:1123419/1‑69 (MQ=255) gtcTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGtt > 1:2292175/1‑69 (MQ=255) gtcTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGtt > 1:790950/1‑69 (MQ=255) gtcTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGtt > 1:3237272/1‑69 (MQ=255) gtcTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGtt > 1:1952156/1‑69 (MQ=255) cTGATTGTTATCGAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGa < 1:2300043/55‑1 (MQ=255) cTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTc > 1:2548505/1‑69 (MQ=255) cTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTc > 1:1885181/1‑69 (MQ=255) tGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTc > 1:2737415/1‑68 (MQ=255) aTTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCg > 1:66657/1‑69 (MQ=255) tGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc < 1:2029511/66‑1 (MQ=255) tGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc < 1:729245/66‑1 (MQ=255) tGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc < 1:2510448/66‑1 (MQ=255) tGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc < 1:1996435/66‑1 (MQ=255) tGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc < 1:2117219/66‑1 (MQ=255) tGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCg < 1:421408/67‑1 (MQ=255) tGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCg < 1:1840693/67‑1 (MQ=255) tGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCg < 1:1828050/67‑1 (MQ=255) aTCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGc < 1:2407420/41‑1 (MQ=255) gagaTGAACCCGCGCGTGTCGCGTTCGTCGGCGCTGGCCTCGAAGGCCACCGGCTTCCCGATCGCGaa < 1:2421518/65‑1 (MQ=255) gAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGAAAGCCACCGGCTTcc > 1:257960/1‑58 (MQ=255) gAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGAAAGCCACCGGCTTCCCGATTGCCaaa > 1:1917441/1‑69 (MQ=255) gAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGAAAGCCACCGGCTTCCCGATTGCCaaa > 1:2833375/1‑69 (MQ=255) gAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGAAAGCCACCGGCTTCCCGATTGCCaaa > 1:3089491/1‑69 (MQ=255) gAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGAAAGCCACCGGCTTCCCGATTGCCaaa > 1:3278118/1‑69 (MQ=255) gAAATGAACCCGCGTGTGTCGCGTTCTTCGGCGCTGGCGTCGAAAGCCACCGGCTTCCCGATTGCCaaa > 1:3304011/1‑69 (MQ=255) gAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTaa < 1:601925/68‑1 (MQ=255) | CAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAA > minE/29347‑29457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |