Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 250,366 C→T 23.7% G304G (GGC→GGT proY → predicted cryptic proline transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE250,3660CT23.7% 150.5 / 35.2 113G304G (GGC→GGTproYpredicted cryptic proline transporter
Reads supporting (aligned to +/- strand):  ref base C (24/62);  new base T (11/16);  total (35/78)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAA  >  minE/250306‑250427
                                                            |                                                             
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:3005784/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:3359686/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:1635383/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:751583/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:1842531/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:2678705/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:1938408/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:2947938/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:1984688/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:2047092/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:2050104/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:309249/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:676547/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:2999128/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:2265814/1‑69 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:2330091/1‑69 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:560182/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:2366140/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:2479862/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:2986039/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:519647/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:1377253/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:2900010/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:1169848/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:2877380/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:1104472/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:335905/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:1378175/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:1498290/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:1499010/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:2761076/69‑1 (MQ=255)
cATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       <  1:928919/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:1235696/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:1872881/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:418088/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:2508668/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:1152874/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:1704743/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:2538267/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:1439530/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:2779779/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:1262630/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:382741/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:1339805/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:1818332/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:707749/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:1386951/69‑1 (MQ=255)
 aTTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGt                                                      <  1:1981753/69‑1 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTATGCCg                                                       >  1:3129582/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:2858723/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:1952290/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:2528576/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:3350927/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:2198493/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:1875764/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:794013/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:794951/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:835610/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:121165/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:1196674/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:1170380/1‑65 (MQ=255)
    cTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCg                                                       >  1:3197365/1‑65 (MQ=255)
                            tCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGc                           <  1:2193821/69‑1 (MQ=255)
                            tCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGc                           <  1:3354541/69‑1 (MQ=255)
                            tCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGc                           <  1:266624/69‑1 (MQ=255)
                            tCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGc                           <  1:239655/69‑1 (MQ=255)
                            tCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGc                           <  1:1417251/69‑1 (MQ=255)
                            tCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGc                           <  1:114344/69‑1 (MQ=255)
                                tggcGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:1780375/65‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:364392/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:526475/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:572431/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:98053/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:3322298/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:2551091/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:1836444/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:2223553/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:1581719/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:2531160/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:1532492/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:2929059/68‑1 (MQ=255)
                                tGTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:3169123/68‑1 (MQ=255)
                                 gTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCt                                                 >  1:1527362/1‑42 (MQ=38)
                                 gTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCAGGGTATGGCAGAGCAGGGCAgcgc                      >  1:271504/1‑69 (MQ=255)
                                 gTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgcgc                      >  1:91506/1‑69 (MQ=255)
                                 gTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgcgc                      >  1:1581313/1‑69 (MQ=255)
                                 gTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgcgc                      >  1:2532720/1‑69 (MQ=255)
                                 gTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgcgc                      >  1:2996222/1‑69 (MQ=255)
                                 gTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgcgc                      >  1:3037329/1‑69 (MQ=255)
                                 gTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgcgc                      >  1:1755695/1‑69 (MQ=255)
                                 gTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgcgc                      >  1:59332/1‑69 (MQ=255)
                                 gTCGGCAATTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgagc                      >  1:1317059/1‑69 (MQ=255)
                                        aTTAACAGTGACGTATTTGGTGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAgc                        <  1:384367/60‑1 (MQ=255)
                                           aaCAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAAtt            >  1:2507843/1‑69 (MQ=255)
                                           aaCAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAAtt            >  1:2656967/1‑69 (MQ=255)
                                           aaCAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAAtt            >  1:2756722/1‑69 (MQ=255)
                                           aaCAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAAt             >  1:2212796/1‑68 (MQ=255)
                                                     tATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCaaaa  >  1:2331700/1‑69 (MQ=255)
                                                     tATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCaaaa  >  1:3226267/1‑69 (MQ=255)
                                                     tATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCaaaa  <  1:3174325/69‑1 (MQ=255)
                                                            |                                                             
CATTCTTAACTTTGTTGTGCTGACTGCTTCGCTGTCGGCAATTAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAA  >  minE/250306‑250427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: