Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,894,755 G→A 48.9% A266A (GCG→GCA ytfT → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,894,7550GA48.9% ‑3.1 / 38.4 45A266A (GCG→GCAytfTpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (7/16);  new base A (17/5);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 2.65e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGG  >  minE/2894692‑2894818
                                                               |                                                               
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGTggtggt                                                            <  1:437361/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCgttggt                                                            <  1:3001843/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:2558162/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:997555/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:581998/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:368106/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:3306235/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:2335640/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:2057490/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:198350/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:1081802/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:1231523/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:1572996/69‑1 (MQ=255)
ggATATTCGCGGTGCCGATGCCAACAACGCAGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:1736058/69‑1 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAgtggtg                                                           >  1:1121707/1‑68 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:2815803/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:106766/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:977336/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:865822/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:794272/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:332549/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:3200264/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:3137143/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:1023118/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:2471152/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:2352204/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:1730366/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:2253770/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:1910130/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:1609118/1‑69 (MQ=255)
  atatTCGCGGTGCCGATGCCAACAATGCCGGATTATGGCTGGAGCTGGACGCCATTCTTGCAGTGGTGa                                                          >  1:2093381/1‑69 (MQ=255)
            tGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGcggcgg                                                <  1:150184/69‑1 (MQ=255)
            tGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGcggcgg                                                <  1:957527/69‑1 (MQ=255)
             gCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:2304190/56‑1 (MQ=255)
             gCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:956958/56‑1 (MQ=255)
             gCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:656250/56‑1 (MQ=255)
             gCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:1951427/56‑1 (MQ=255)
             gCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                            <  1:2649065/56‑1 (MQ=255)
                 aTGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGGGATCGCt                                          <  1:2670426/69‑1 (MQ=255)
                  tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:1696983/69‑1 (MQ=255)
                  tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:1801296/69‑1 (MQ=255)
                  tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:2782526/69‑1 (MQ=255)
                  tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:2093315/69‑1 (MQ=255)
                  tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:772444/69‑1 (MQ=255)
                  tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:3291065/69‑1 (MQ=255)
                  tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:2122661/69‑1 (MQ=255)
                  tGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:1075581/69‑1 (MQ=255)
                  tGCCAACAACGCTGCGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCt                                          <  1:3025831/69‑1 (MQ=255)
                         aacGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATgggc                                   >  1:2537674/1‑69 (MQ=255)
                         aacGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATgggc                                   >  1:1271174/1‑69 (MQ=255)
                                    aTGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTaa                        <  1:2920490/69‑1 (MQ=255)
                                    aTGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTaa                        <  1:668065/69‑1 (MQ=255)
                                              ctggaCGCCATTCTTGCAGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCtt              <  1:3365155/69‑1 (MQ=255)
                                              ctggaCGCCATTCTTGCAGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCtt              <  1:2020629/69‑1 (MQ=255)
                                              ctggaCGCCATTCTTGCAGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCtt              <  1:302599/69‑1 (MQ=255)
                                              ctggaCGCCATTCTTGCAGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCtt              <  1:2943426/69‑1 (MQ=255)
                                               tggaCGCCATTCTTGCAGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTGCtt              <  1:1772031/68‑1 (MQ=255)
                                                   cGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCggt         <  1:3249630/69‑1 (MQ=255)
                                                   cGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCggt         <  1:1820347/69‑1 (MQ=255)
                                                          ctcGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCttt                          >  1:524913/1‑45 (MQ=255)
                                                          ctcGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCttt                          >  1:2644203/1‑45 (MQ=255)
                                                          ctcGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCttt                          >  1:2369292/1‑45 (MQ=255)
                                                          ctcGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGgtggtgtg   >  1:236154/1‑66 (MQ=255)
                                                          ctcGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTgggg  >  1:487790/1‑69 (MQ=255)
                                                               |                                                               
GGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGG  >  minE/2894692‑2894818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: