| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,895,585 | C→T | 25.6% | R207C (CGC→TGC) | yjfF → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,895,585 | 0 | C | T | 25.6% | 79.0 / 21.9 | 66 | R207C (CGC→TGC) | yjfF | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (45/4); new base T (6/11); total (51/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
CAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGT > minE/2895528‑2895646 | cAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCgcagc > 1:479066/1‑63 (MQ=255)cAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCgcagc > 1:3211994/1‑63 (MQ=255)cAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCgcagc > 1:2703342/1‑63 (MQ=255)cAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCgcagc > 1:835088/1‑63 (MQ=255)cAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCgcagc > 1:252720/1‑63 (MQ=255)cAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCgcagc > 1:252082/1‑63 (MQ=255)cAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCgcagc > 1:1608217/1‑63 (MQ=255) aGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACt > 1:3105076/1‑68 (MQ=255) aGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTa > 1:3287985/1‑69 (MQ=255) aGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTa > 1:2471522/1‑69 (MQ=255) aGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTa > 1:2410567/1‑69 (MQ=255) aGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGAAGGGGATTTCCACTCGCAGCACCACTa > 1:1632983/1‑69 (MQ=255) cGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCa > 1:1111166/1‑68 (MQ=255) cGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCa > 1:428860/1‑68 (MQ=255) cGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCa > 1:3340025/1‑68 (MQ=255) cGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCa > 1:2099075/1‑68 (MQ=255) cGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCa > 1:1909067/1‑68 (MQ=255) cGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCa > 1:179854/1‑68 (MQ=255) ccATTGGCGGCAACGCAACGTCGGCAAACCTGATGGGGATTTTCACTTGCAGCACCACTATTCGCAtt < 1:842929/68‑1 (MQ=255) ccATTGGCGGCAACGCAACGTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCAtt < 1:1943777/68‑1 (MQ=255) ccATTGGCGGCAACGCAACGTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCAtt < 1:1240496/68‑1 (MQ=255) ccATTGGCGGCAACGCAACGTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCAtt < 1:987600/68‑1 (MQ=255) ccATTGGCGGCAACGCAACGTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCAtt < 1:752191/68‑1 (MQ=255) ccATTGGCGGCAACGCAACGTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCAtt < 1:1484414/68‑1 (MQ=255) ccATTGGCGGCAACGCAACGTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCAtt < 1:912303/68‑1 (MQ=255) ccATTGGCGGCAACGCAACGTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCAtt < 1:1578840/68‑1 (MQ=255) ccATTGGCGGCAACGCAACGTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCAtt < 1:2474923/68‑1 (MQ=255) ccATTGGCAGCAACGCAACGTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCAtt < 1:2119448/68‑1 (MQ=255) cATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCTCATTta < 1:443221/69‑1 (MQ=255) cATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTta < 1:2115736/69‑1 (MQ=255) ggcggcAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTatat > 1:630503/1‑68 (MQ=255) ggcggcAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTatat > 1:1901157/1‑68 (MQ=255) ggcggcAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATg > 1:819728/1‑69 (MQ=255) caacgcaacgTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCtctc < 1:1833623/69‑1 (MQ=255) caacgcaacgTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCtctc < 1:348295/69‑1 (MQ=255) caacgcaacgTCGGCAACCCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCATTTATATGc > 1:2966348/1‑65 (MQ=255) caacgcaacgTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCATTTatat > 1:1127501/1‑63 (MQ=255) caacgcaacgTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCATTTATATg > 1:1320934/1‑64 (MQ=255) caacgcaacgTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCATTTATATGc > 1:489141/1‑65 (MQ=255) caacgcaacgTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCATTTATATGc > 1:2582180/1‑65 (MQ=255) caacgcaacgTCGGCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCATTTATATGc > 1:2447364/1‑65 (MQ=255) aacgcaacgTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTcc > 1:136321/1‑69 (MQ=255) aacgcaacgTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTcc > 1:863444/1‑69 (MQ=255) aacgcaacgTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTcc > 1:2297135/1‑69 (MQ=255) gcaacgTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCAc > 1:2082115/1‑68 (MQ=255) gcaacgTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCAc > 1:344619/1‑68 (MQ=255) gcaacgTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCAc > 1:609906/1‑68 (MQ=255) gcaacgTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCAc > 1:503696/1‑68 (MQ=255) caacgTCGGCCAATCTGATGGCGATTTCCACTCGCAGCACCACAATTCGCATTTATATGCTCTCCACCg > 1:2284135/1‑69 (MQ=255) ggCAAACCTGATGGGGATTTCCACTTGCAGCACCACTATTCGCAtt < 1:2984543/46‑1 (MQ=255) ttCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGt > 1:72572/1‑69 (MQ=255) ttCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGt > 1:924264/1‑69 (MQ=255) ttCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGt > 1:1117290/1‑69 (MQ=255) ttCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGt > 1:1556380/1‑69 (MQ=255) ttCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGt > 1:1552348/1‑69 (MQ=255) tCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCgg > 1:495048/1‑44 (MQ=255) tCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCgg > 1:492055/1‑44 (MQ=255) tCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCgg > 1:3378267/1‑44 (MQ=255) tCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCgg > 1:2932799/1‑44 (MQ=255) tCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCgg > 1:289199/1‑44 (MQ=255) tCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCgg > 1:796575/1‑44 (MQ=255) tCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCgg > 1:2726591/1‑44 (MQ=255) tCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCgg > 1:2645832/1‑44 (MQ=255) tCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCgg > 1:218585/1‑44 (MQ=255) tCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCgg > 1:2182145/1‑44 (MQ=255) tCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCgg > 1:1337636/1‑44 (MQ=255) | CAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGT > minE/2895528‑2895646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |