| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,895,677 | G→A | 29.9% | A237A (GCG→GCA) | yjfF → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,895,677 | 0 | G | A | 29.9% | 58.6 / 24.5 | 60 | A237A (GCG→GCA) | yjfF | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (5/37); new base A (8/10); total (13/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.33e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTAATTGGCGGTACGCT > minE/2895610‑2895736 | tGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATgcg > 1:2334921/1‑68 (MQ=255) ctctCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTg > 1:1201823/1‑69 (MQ=255) ccGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTggcggg > 1:298444/1‑67 (MQ=255) ccGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTggcggg > 1:2175298/1‑67 (MQ=255) ccGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTggcggg > 1:392964/1‑67 (MQ=255) ccGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTggcggg > 1:1680916/1‑67 (MQ=255) ccGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTggcggg > 1:285180/1‑67 (MQ=255) ccGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTggcggg > 1:1531941/1‑67 (MQ=255) ccGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTggcggg > 1:1142218/1‑67 (MQ=255) ccGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTggcgg > 1:2895436/1‑66 (MQ=255) aCTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAgg < 1:1232680/69‑1 (MQ=255) gCAATGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGgtgt > 1:3124268/1‑68 (MQ=255) gCAACGCTGGCTGGGATTGTCTTCTCGATTTATACACAGGCCGGATATgcgc > 1:781624/1‑52 (MQ=255) gCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGgtgt > 1:2288721/1‑68 (MQ=255) gCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGgtgt > 1:734933/1‑68 (MQ=255) gCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGgtgt > 1:2594282/1‑68 (MQ=255) aaCGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGa < 1:2861024/69‑1 (MQ=255) cTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTg < 1:2460705/69‑1 (MQ=255) cTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTg < 1:1232739/69‑1 (MQ=255) cTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTg < 1:2044959/69‑1 (MQ=255) cTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTg < 1:579794/69‑1 (MQ=255) cTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTg < 1:1659966/69‑1 (MQ=255) cTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTg < 1:635469/69‑1 (MQ=255) cTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTg < 1:1541379/69‑1 (MQ=255) cTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTg < 1:1450392/69‑1 (MQ=255) gggATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCt < 1:1038713/69‑1 (MQ=255) gggATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCt < 1:3142446/69‑1 (MQ=255) gggATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCt < 1:403894/69‑1 (MQ=255) gggATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCt < 1:1569678/69‑1 (MQ=255) gggATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCt < 1:847379/69‑1 (MQ=255) ggATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCt < 1:3081023/68‑1 (MQ=255) gATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:47707/69‑1 (MQ=255) gATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:1554451/69‑1 (MQ=255) gATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:286971/69‑1 (MQ=255) gATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:3287232/69‑1 (MQ=255) gATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:355991/69‑1 (MQ=255) gATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:2843585/69‑1 (MQ=255) gATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:1068374/69‑1 (MQ=255) gATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:1114820/69‑1 (MQ=255) gATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:2486681/69‑1 (MQ=255) gATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:2424244/69‑1 (MQ=255) gATTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:1642826/69‑1 (MQ=255) aTTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:2940305/68‑1 (MQ=255) aTTGTCTTCTCGATTTATACCCAGGCCGGATATGCACTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:323173/68‑1 (MQ=255) gTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAgg < 1:2553923/47‑1 (MQ=255) gTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAgg < 1:1881509/47‑1 (MQ=255) tttATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTa < 1:1666624/68‑1 (MQ=255) tttATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTa < 1:1230612/68‑1 (MQ=255) tttATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTa < 1:2356214/68‑1 (MQ=255) tttATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTa < 1:214442/68‑1 (MQ=255) tttATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTa < 1:632669/68‑1 (MQ=255) cAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTAATTGGCgg < 1:688614/68‑1 (MQ=255) cAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTAATTGGCgg < 1:932185/68‑1 (MQ=255) cAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTAATTGGCgg < 1:2913298/68‑1 (MQ=255) cAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTAATTGGCgg < 1:3222114/68‑1 (MQ=255) cAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTAATTGGCgg < 1:3212880/68‑1 (MQ=255) cAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTAATTGGCgg < 1:2895991/68‑1 (MQ=255) cAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTAATTGGCgg < 1:2923530/68‑1 (MQ=255) cGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTAATTGGCGGTACGCt < 1:426572/69‑1 (MQ=255) cGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTAATTGGCGGTACGCt < 1:926733/69‑1 (MQ=255) ggATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTAt < 1:1027745/41‑1 (MQ=255) atGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCt < 1:2044579/35‑1 (MQ=255) | TGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGTAATTGGCGGTACGCT > minE/2895610‑2895736 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |