| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,903,226 | T→C | 53.2% | S273S (TCA→TCG) | nrdD ← | anaerobic ribonucleoside‑triphosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,903,226 | 0 | T | C | 53.2% | 9.0 / 65.6 | 79 | S273S (TCA→TCG) | nrdD | anaerobic ribonucleoside‑triphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base T (26/11); new base C (28/14); total (54/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
CTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGA > minE/2903184‑2903290 | cTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCttt < 1:1025439/69‑1 (MQ=255)cTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCttt < 1:3278614/69‑1 (MQ=255)cTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCttt < 1:621343/69‑1 (MQ=255)cTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCttt < 1:684164/69‑1 (MQ=255)cTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCttt < 1:1573063/69‑1 (MQ=255)cTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCttt < 1:1467730/69‑1 (MQ=255) tGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTg > 1:2551173/1‑69 (MQ=255) tGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTg > 1:3294978/1‑69 (MQ=255) tGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTg > 1:2768669/1‑69 (MQ=255) tGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTg > 1:846557/1‑69 (MQ=255) tGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTg > 1:81764/1‑69 (MQ=255) tGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTg > 1:891439/1‑69 (MQ=255) aCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGAt > 1:11794/1‑69 (MQ=255) aCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGAt > 1:1623591/1‑69 (MQ=255) aCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGAt > 1:2952362/1‑69 (MQ=255) aCCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGAt > 1:3225997/1‑69 (MQ=255) cGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGAt < 1:3081422/67‑1 (MQ=255) gtgtTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAg > 1:2988975/1‑69 (MQ=255) tgtTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc > 1:1711875/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGTTACGAGAGTTAGCGTAGcc > 1:826740/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:2944615/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:3013902/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:2887426/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:2855595/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:3287095/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:266952/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:3305356/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:1219124/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:3383766/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:2428882/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:2230939/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:852117/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:1977072/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:422011/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:473954/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:851720/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:575154/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:924106/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:701712/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:2402322/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:791284/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:1470325/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:16131/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:11374/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGc > 1:1282757/1‑68 (MQ=255) gtTTACTTCGTACTACAGCGACTGGAAGGCATCGTAGCACTCTTTGATCGTACGAGAGTTAGCGTAGcc > 1:723793/1‑69 (MQ=255) gtTTACTTCGAACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:3147553/1‑69 (MQ=255) tACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCttt < 1:1028251/42‑1 (MQ=255) tACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCttt < 1:2576456/42‑1 (MQ=255) tACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCttt < 1:2499195/42‑1 (MQ=255) tACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTc > 1:2921489/1‑50 (MQ=255) tACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTc > 1:999483/1‑69 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:597096/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1349412/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1659195/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:126753/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1708827/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:651332/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:12670/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1862862/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1161713/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:483424/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1038472/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1937609/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:953349/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:2576157/68‑1 (MQ=255) cTCCAGTGACTTGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:2815910/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:863879/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1821228/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1028205/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1376271/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1434078/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1683010/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:641670/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1873364/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1876762/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:2545112/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:2734640/1‑69 (MQ=255) ccAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGa < 1:494129/69‑1 (MQ=255) | CTGACCGTTGGCGGTGTGCAGGGTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGA > minE/2903184‑2903290 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |