| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,903,259 | T→A | 46.3% | R262R (CGA→CGT) | nrdD ← | anaerobic ribonucleoside‑triphosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,903,259 | 0 | T | A | 46.3% | 19.9 / 141.6 | 121 | R262R (CGA→CGT) | nrdD | anaerobic ribonucleoside‑triphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base T (29/36); new base A (41/15); total (70/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
Only 100 of 129 total aligned reads displayed.
GTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGATGTTTGTTGTA > minE/2903206‑2903327 | gtgtTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAg > 1:2988975/1‑69 (MQ=255) tgtTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc > 1:1711875/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGTTACGAGAGTTAGCGTAGcc > 1:826740/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:3383766/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:2428882/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:2402322/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:266952/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:2855595/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:2887426/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:16131/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:3013902/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:3287095/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:3305356/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:422011/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:2230939/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:575154/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:701712/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:1219124/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:791284/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:851720/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:852117/1‑69 (MQ=255) gtTTACTTCGTACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:924106/1‑69 (MQ=255) gtTTACTTCGTACTACAGCGACTGGAAGGCATCGTAGCACTCTTTGATCGTACGAGAGTTAGCGTAGcc > 1:723793/1‑69 (MQ=255) gtTTACTTCGAACTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGcc > 1:3147553/1‑69 (MQ=255) tACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTc > 1:2921489/1‑50 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1937609/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1038472/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:2576157/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:483424/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:953349/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1161713/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:12670/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:126753/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1349412/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1862862/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1659195/68‑1 (MQ=255) aCTCCAGCGACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1708827/68‑1 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1376271/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1028205/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:863879/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:641670/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1434078/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1683010/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:2734640/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:2545112/1‑69 (MQ=255) cTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCgg > 1:1873364/1‑69 (MQ=255) ccAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGa < 1:494129/69‑1 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc < 1:3160495/49‑1 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc < 1:339627/49‑1 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc < 1:2015213/49‑1 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc < 1:2311643/49‑1 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc < 1:1172598/49‑1 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc < 1:847428/49‑1 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc < 1:2585359/49‑1 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGtt > 1:1855437/1‑68 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTc > 1:821371/1‑69 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTc > 1:555539/1‑69 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTc > 1:492272/1‑69 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTc > 1:469807/1‑69 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTc > 1:2092518/1‑69 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTc > 1:1126952/1‑69 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTc > 1:2555338/1‑69 (MQ=255) gACTGGAAGGCATCGTAGCACTCTTTGATGGTACGAGAGTTAGCGTAGCCTTCGGCGTCC‑‑GATGTTCCa > 1:1355188/1‑69 (MQ=255) aCTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTcc > 1:2268286/1‑69 (MQ=255) aGGCATCGTAGCACTCTTTTATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCActc < 1:442067/67‑1 (MQ=255) aGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCActc < 1:2427500/67‑1 (MQ=255) aGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCActc < 1:1754781/67‑1 (MQ=255) aGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCActc < 1:640643/67‑1 (MQ=255) aGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCActc < 1:34782/67‑1 (MQ=255) ggCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGc < 1:2679703/41‑1 (MQ=255) gTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGt > 1:1439006/1‑69 (MQ=255) gTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGt > 1:3422291/1‑69 (MQ=255) gTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGt > 1:3312357/1‑69 (MQ=255) gTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGt > 1:600151/1‑69 (MQ=255) gCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGTGGTCtt < 1:5337/69‑1 (MQ=255) gCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCtt < 1:3050542/69‑1 (MQ=255) gCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCtt < 1:2881448/69‑1 (MQ=255) gCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCtt < 1:3220564/69‑1 (MQ=255) gCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCtt < 1:1925689/69‑1 (MQ=255) gCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCtt < 1:1096237/69‑1 (MQ=255) gCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCtt < 1:133245/69‑1 (MQ=255) gCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCtt < 1:2975840/69‑1 (MQ=255) gCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCtt < 1:3342761/69‑1 (MQ=255) cACTCTTTTATGGTACGAGAGTTAGCGTAGCCTTCGGCGTcc < 1:1429397/42‑1 (MQ=255) aCTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGc < 1:1573006/69‑1 (MQ=255) aCTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCAGGGATGTTCCACTCTTCTGCGGTCTTGc < 1:2068620/69‑1 (MQ=255) ctTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGAt > 1:340467/1‑69 (MQ=255) ctTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGAt > 1:1258251/1‑69 (MQ=255) ctTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGAt > 1:2975322/1‑69 (MQ=255) tttGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCTGTCTTGCGAtg > 1:697266/1‑69 (MQ=255) tttGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGAtg > 1:1968272/1‑69 (MQ=255) tttGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGAtg > 1:804218/1‑69 (MQ=255) tGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGAtgtttg < 1:60121/68‑1 (MQ=255) tCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGATGTTTGTTGTa < 1:1860895/69‑1 (MQ=255) tCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGATGTTTGTTGTa < 1:176522/69‑1 (MQ=255) tCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGATGTTTGTTGTa < 1:1774887/69‑1 (MQ=255) tCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGATGTTTGTTGTa < 1:1182091/69‑1 (MQ=255) tCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGATGTTTGTTGTa < 1:2293286/69‑1 (MQ=255) tCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGATGTTTGTTGTa < 1:494339/69‑1 (MQ=255) tCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGATGTTTGTTGTa < 1:1629195/69‑1 (MQ=255) | GTGTTTACTTCGTACTCCAGTGACTGGAAGGCATCGTAGCACTCTTTGATGGTTCGAGAGTTAGCGTAGCCTTCGGCGTCCGGGATGTTCCACTCTTCTGCGGTCTTGCGATGTTTGTTGTA > minE/2903206‑2903327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |