| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,903,574 | G→A | 32.6% | G157G (GGC→GGT) | nrdD ← | anaerobic ribonucleoside‑triphosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,903,574 | 0 | G | A | 32.6% | 84.0 / 50.8 | 98 | G157G (GGC→GGT) | nrdD | anaerobic ribonucleoside‑triphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base G (45/21); new base A (14/18); total (59/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01 | |||||||||||
CGATCAACATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACG > minE/2903510‑2903640 | cGATCAACATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCa > 1:209494/1‑68 (MQ=255)cGATCAACATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCAc > 1:2264262/1‑69 (MQ=255)cGATCAACATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCAc > 1:657954/1‑69 (MQ=255)cGATCAACATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCAc > 1:1217699/1‑69 (MQ=255)cGATCAACATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCAc > 1:1825794/1‑69 (MQ=255)cGATCAACATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCAc > 1:2841693/1‑69 (MQ=255)cGATCAACATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCAc > 1:3062829/1‑69 (MQ=255) cAACATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTc < 1:1701801/69‑1 (MQ=255) cAACATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTc < 1:3120702/69‑1 (MQ=255) aaCATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCtga < 1:2362118/69‑3 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGc > 1:195631/1‑59 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:300985/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:2270793/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:2094101/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:2439716/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:2707275/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:3375480/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:965125/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:1097449/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:813188/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:1161326/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:1324949/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:397836/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:1419815/1‑69 (MQ=255) cATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCAtg > 1:3354487/1‑69 (MQ=255) gAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGcac < 1:2942005/69‑1 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:2732617/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:1005605/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:3346024/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:2827846/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:266642/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:490738/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:2976885/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:93032/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:2713183/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:3028013/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:1177382/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:1260329/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:2046734/1‑69 (MQ=255) aaCGGTGAGTAATCGAGATCCTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgc > 1:1049497/1‑69 (MQ=255) aCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCAc > 1:2457578/1‑39 (MQ=255) aCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTg > 1:2797421/1‑52 (MQ=255) aCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTg > 1:1482052/1‑52 (MQ=255) aCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTg < 1:1740041/52‑1 (MQ=255) aCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTg < 1:2267731/52‑1 (MQ=255) aCGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTg < 1:412635/52‑1 (MQ=255) ggTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGgcagc < 1:3340204/69‑1 (MQ=255) tAATCGAGATCGTGATAGTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGc > 1:1415074/1‑69 (MQ=255) tAATCGAGATCGTGATAGTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGc > 1:3424283/1‑69 (MQ=255) tAATCGAGATCGTGATAGTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGc > 1:3058870/1‑69 (MQ=255) tAATCGAGATCGTGATAGTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGc > 1:727157/1‑69 (MQ=255) tAATCGAGATCGTGATAGTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGc > 1:2842345/1‑69 (MQ=255) aaTCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGcac < 1:1453693/46‑1 (MQ=255) cGAGATCGTGATAGTGAATATCGCCACGCTCATGTTCCTGCACCACGTCACGCGGCAGCAGGTGCTGa < 1:752797/68‑1 (MQ=255) cGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGa < 1:1717780/68‑1 (MQ=255) cGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGa < 1:2504187/68‑1 (MQ=255) cGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGa < 1:3322060/68‑1 (MQ=255) cGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGAc > 1:2795689/1‑69 (MQ=255) cGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCAt < 1:608185/69‑1 (MQ=255) aGTGAATATCGCCCCGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAgtg > 1:1134054/1‑67 (MQ=255) aGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAgtg > 1:1130549/1‑67 (MQ=255) aGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAgtg > 1:2072270/1‑67 (MQ=255) aGTGAATATCGCCACGCTCATCTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGCCGTGCATAgt > 1:147674/1‑66 (MQ=255) gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCt > 1:2863283/1‑53 (MQ=255) gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:2057744/1‑69 (MQ=255) gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:259270/1‑69 (MQ=255) gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:2313796/1‑69 (MQ=255) gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:74047/1‑69 (MQ=255) gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:3107704/1‑69 (MQ=255) gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:2043813/1‑69 (MQ=255) gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:767844/1‑69 (MQ=255) gTGAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGGCGTGCATAGTGttt > 1:1603377/1‑69 (MQ=255) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:681511/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:632386/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:619488/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:781653/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:610409/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:604843/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:27521/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:1351355/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:367212/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:2478761/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:3392844/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:1449622/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:328785/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:3283927/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:3118191/44‑1 (MQ=38) gAATATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:2724906/44‑1 (MQ=38) gAATATCACCACGTTCGGGTGCCTGCACCACGTCACGCGgcagc < 1:1534225/44‑1 (MQ=25) aaTATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGc > 1:1531806/1‑69 (MQ=255) aaTATCACCACGTTCGTGTGCCTGCACCACGTCACGCGgcagc < 1:500122/43‑1 (MQ=37) atCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCAc < 1:1423845/69‑1 (MQ=255) tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:2119169/69‑1 (MQ=255) tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:1561320/69‑1 (MQ=255) tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:857744/69‑1 (MQ=255) tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:1491156/69‑1 (MQ=255) tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:2642155/69‑1 (MQ=255) tCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACg < 1:2432990/69‑1 (MQ=255) | CGATCAACATGCAGTTGAACATCGGGAAGAACGGTGAGTAATCGAGATCGTGATAGTGAATATCGCCACGCTCATGTGCCTGCACCACGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACG > minE/2903510‑2903640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |