| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,906,404 | T→A | 39.3% | A387A (GCA→GCT) | treB ← | fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,906,404 | 0 | T | A | 39.3% | 72.8 / 111.2 | 117 | A387A (GCA→GCT) | treB | fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
| Reads supporting (aligned to +/- strand): ref base T (33/38); new base A (23/23); total (56/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
ACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTG > minE/2906344‑2906469 | accagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTc < 1:471320/69‑1 (MQ=255)accagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTc < 1:3187436/69‑1 (MQ=255)accagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTc < 1:1232862/69‑1 (MQ=255) ccagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTc < 1:2973255/68‑1 (MQ=255) atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:3228806/1‑67 (MQ=255) atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:1838635/1‑67 (MQ=255) atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:1956625/1‑67 (MQ=255) atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:666157/1‑67 (MQ=255) atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:1022409/1‑67 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGcc > 1:1303382/1‑68 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:674280/1‑69 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:2258712/1‑69 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:2317671/1‑69 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:328803/1‑69 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:1568588/1‑69 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:457436/1‑69 (MQ=255) cgcgCACAGCATCGGGAAGAGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:1019005/1‑69 (MQ=255) cacaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCg < 1:1531533/69‑1 (MQ=255) cacaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCg < 1:2362312/69‑1 (MQ=255) acaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCg < 1:8956/68‑1 (MQ=255) caGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:3215992/69‑1 (MQ=255) caGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:1287043/69‑1 (MQ=255) aGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:874449/68‑1 (MQ=255) gCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga > 1:2778165/1‑67 (MQ=255) gCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga > 1:3297178/1‑67 (MQ=255) cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGgaga > 1:603789/1‑68 (MQ=255) cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt > 1:2621181/1‑69 (MQ=255) cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt > 1:1772158/1‑69 (MQ=255) cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt > 1:1437456/1‑69 (MQ=255) cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt < 1:10319/69‑1 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:402579/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:2466910/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:2320863/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:2911731/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:2266500/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:1442231/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:3143102/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:849153/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:404390/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:555006/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:1762985/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:1717678/1‑69 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGc > 1:546982/1‑35 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:3005774/1‑54 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:534425/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:3264554/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:3277111/1‑54 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:2845550/1‑54 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:2794604/1‑54 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:3418075/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:2657429/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:1718341/1‑54 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:1372707/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:107721/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:2108906/1‑54 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:2357954/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:2184681/69‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:1426276/69‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:3108588/69‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:2209225/69‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:966132/69‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:2726469/69‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:2569471/69‑1 (MQ=255) atatTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:2044144/46‑1 (MQ=255) tatTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:1245686/53‑1 (MQ=255) tatTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:1832175/53‑1 (MQ=255) tCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGgag < 1:2936930/68‑1 (MQ=255) tCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGgag < 1:2854057/68‑1 (MQ=255) tCAGGTTGATGCCCTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGgag < 1:3145955/68‑1 (MQ=255) gTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGAtct < 1:1057133/68‑1 (MQ=255) gTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGAtct < 1:1053174/68‑1 (MQ=255) ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:2726201/1‑69 (MQ=255) ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:1565207/1‑69 (MQ=255) ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:1750821/1‑69 (MQ=255) ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:1452155/1‑69 (MQ=255) ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:2237150/1‑69 (MQ=255) tGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcgc > 1:2981947/1‑69 (MQ=255) tGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcgc > 1:2268195/1‑69 (MQ=255) tGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGAGGCAGGCACGGAGATCTcgc > 1:1455829/1‑69 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1449906/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:867190/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:414113/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1615740/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1786529/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3431678/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3423736/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3421443/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3371411/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3364368/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3347156/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2686109/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1876738/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1990295/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2071896/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3068068/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2963934/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2501151/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2704103/69‑1 (MQ=255) cATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTTATtgtg < 1:440542/69‑1 (MQ=255) cATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtgtg < 1:1378048/69‑1 (MQ=255) | ACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTG > minE/2906344‑2906469 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |