| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,906,419 | C→T | 33.7% | G382G (GGG→GGA) | treB ← | fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,906,419 | 0 | C | T | 33.7% | 112.1 / 74.5 | 136 | G382G (GGG→GGA) | treB | fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (48/42); new base T (23/23); total (71/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01 | |||||||||||
Only 100 of 152 total aligned reads displayed.
ATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAT > minE/2906357‑2906487 | atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:1838635/1‑67 (MQ=255)atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:3048368/1‑67 (MQ=255)atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa > 1:666157/1‑67 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGcc > 1:1303382/1‑68 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:1736987/1‑69 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:328803/1‑69 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:1568588/1‑69 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:759515/1‑69 (MQ=255) cgcgCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:2317671/1‑69 (MQ=255) cgcgCACAGCATCGGGAAGAGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCa > 1:1019005/1‑69 (MQ=255) cacaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCg < 1:1531533/69‑1 (MQ=255) caGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:1287043/69‑1 (MQ=255) caGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:3215992/69‑1 (MQ=255) aGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:874449/68‑1 (MQ=255) gCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga > 1:2778165/1‑67 (MQ=255) gCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga > 1:1210329/1‑67 (MQ=255) cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt > 1:1437456/1‑69 (MQ=255) cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt < 1:10319/69‑1 (MQ=255) cATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt > 1:1772158/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:2466910/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:2911731/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:3143102/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:2266500/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:1717678/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:402579/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:404390/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:849153/1‑69 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATa > 1:555006/1‑69 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:2794604/1‑54 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:107721/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:2357954/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:2176941/1‑54 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:2657429/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:2717927/1‑54 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:1372707/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:3277111/1‑54 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:534425/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:3418075/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:3264554/54‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:2569471/69‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:3108588/69‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:966132/69‑1 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCg < 1:1426276/69‑1 (MQ=255) tatTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:1832175/53‑1 (MQ=255) tatTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:1245686/53‑1 (MQ=255) tCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGgag < 1:2854057/68‑1 (MQ=255) tCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGgag < 1:2936930/68‑1 (MQ=255) gTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGAtct < 1:1053174/68‑1 (MQ=255) ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:1750821/1‑69 (MQ=255) ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:2726201/1‑69 (MQ=255) ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:321772/1‑69 (MQ=255) ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:2237150/1‑69 (MQ=255) ttGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcg > 1:1452155/1‑69 (MQ=255) tGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTcgc > 1:2981947/1‑69 (MQ=255) tGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACCGAGATCTcgc > 1:1395337/1‑69 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1860471/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1786529/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1876738/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3068068/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3347156/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3350979/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3371411/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1615740/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3423736/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:3431678/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1520685/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1449906/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:1990295/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:867190/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2071896/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2686109/69‑1 (MQ=255) tACATAGCAGGCTCAGTGACTCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATtg < 1:2507899/69‑1 (MQ=255) cATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTTATtgtg < 1:440542/69‑1 (MQ=255) gCAGGCTCTGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTg > 1:554153/1‑69 (MQ=255) gCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCgg > 1:497543/1‑37 (MQ=255) gCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTg > 1:2262724/1‑69 (MQ=255) gCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTg > 1:2075633/1‑69 (MQ=255) gCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTg > 1:2168630/1‑69 (MQ=255) gCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTg > 1:2190398/1‑69 (MQ=255) gCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTg > 1:433398/1‑69 (MQ=255) gCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTg > 1:2245403/1‑69 (MQ=255) ggCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCg < 1:2189114/68‑1 (MQ=255) cAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTgg < 1:249703/69‑1 (MQ=255) cAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTgg < 1:2354199/69‑1 (MQ=255) cAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTgg < 1:832290/69‑1 (MQ=255) gACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAAt > 1:491460/1‑69 (MQ=255) gACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAAt > 1:2015228/1‑69 (MQ=255) gACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAAt > 1:2993861/1‑69 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:1021776/69‑1 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:141981/69‑1 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:785360/69‑1 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:652558/69‑1 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:460464/69‑1 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:1677560/69‑1 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:1802614/69‑1 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:2848181/69‑1 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:2362394/69‑1 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:2306194/69‑1 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:2287183/69‑1 (MQ=255) cccAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAt < 1:2280088/69‑1 (MQ=255) | ATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGCGTTCATTGTGCTTGCGGCTGGAAATGAT > minE/2906357‑2906487 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |