| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,906,647 | T→C | 23.0% | A306A (GCA→GCG) | treB ← | fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,906,647 | 0 | T | C | 23.0% | 97.7 / 24.3 | 78 | A306A (GCA→GCG) | treB | fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
| Reads supporting (aligned to +/- strand): ref base T (36/24); new base C (4/14); total (40/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCC > minE/2906579‑2906709 | tCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAAtgct > 1:3177116/1‑69 (MQ=255)tCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAAtgct > 1:1789382/1‑69 (MQ=255)tCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAAtgct > 1:890681/1‑69 (MQ=255) gCGTGGTCTGGTGTACACCGGTGATCACCAGCGTGGCGTACATGAAGCCAAACAATGCTGCGCCAATCg < 1:953284/69‑1 (MQ=255) gCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCg < 1:966654/69‑1 (MQ=255) gCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCg < 1:2634488/69‑1 (MQ=255) cGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCgg > 1:1711542/1‑69 (MQ=255) cGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCgg > 1:603500/1‑69 (MQ=255) cGTGGTCTGGTGTACACCGGTGATAACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCgg > 1:417143/1‑69 (MQ=255) gTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg > 1:3348724/1‑67 (MQ=255) gTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg > 1:506675/1‑67 (MQ=255) gTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg > 1:432859/1‑67 (MQ=255) gTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg > 1:1937784/1‑67 (MQ=255) gTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg > 1:1008097/1‑67 (MQ=255) gTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg > 1:2805770/1‑67 (MQ=255) gTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg > 1:3113945/1‑67 (MQ=255) gTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg > 1:308921/1‑67 (MQ=255) gtACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCt < 1:3376163/67‑1 (MQ=255) ggTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGtc > 1:264242/1‑67 (MQ=255) ggTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGtc > 1:994829/1‑67 (MQ=255) ggTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGtc > 1:590032/1‑67 (MQ=255) ggTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGtc > 1:599409/1‑67 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGt < 1:1490016/68‑1 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGt < 1:1762737/68‑1 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGt < 1:2026467/68‑1 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGt < 1:2072943/68‑1 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGt < 1:3407885/68‑1 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGt < 1:249509/68‑1 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTg > 1:3198778/1‑69 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTg > 1:1398832/1‑69 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTg > 1:2600802/1‑69 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTg > 1:3343049/1‑69 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTg > 1:3215767/1‑69 (MQ=255) cACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTg > 1:1506518/1‑69 (MQ=255) aCCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGt < 1:1440602/67‑1 (MQ=255) aCCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGa < 1:1645359/69‑1 (MQ=255) aCCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGa < 1:3079214/69‑1 (MQ=255) aCCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGa < 1:63899/69‑1 (MQ=255) aCCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGa < 1:2189647/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:2915679/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:893299/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:959150/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:564453/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:1054637/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:530609/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:3066002/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:3039010/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:2907837/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:1209650/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:1214494/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:256711/69‑1 (MQ=255) gCGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCAGTCATCAGGTGACGTACCGCa < 1:808697/69‑1 (MQ=255) cGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:3342616/68‑1 (MQ=255) cGTACAGGAAGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:3050690/68‑1 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCaaa > 1:734632/1‑36 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcatca > 1:1466364/1‑50 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCaa > 1:2320379/1‑65 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:84123/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:1475674/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:1473963/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:758974/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:165020/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:1452554/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:1219825/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:1153382/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:3056889/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc < 1:1720239/69‑1 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:2391740/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:2394054/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:2474775/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:3294594/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:3282145/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:2841645/1‑69 (MQ=255) cAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 1:3073238/1‑69 (MQ=255) aGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGc < 1:1459041/69‑1 (MQ=255) aGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGc < 1:2718517/69‑1 (MQ=255) aGCCAAACAATGCCGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa < 1:2856040/59‑1 (MQ=255) gCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGcc < 1:817457/69‑1 (MQ=255) cAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGcc < 1:1542620/69‑1 (MQ=255) cAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGcc < 1:1464047/69‑1 (MQ=255) cAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGcc < 1:1313146/69‑1 (MQ=255) | TCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCC > minE/2906579‑2906709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |