BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,911,210	C→T	48.0%	N734N (AAC→AAT)	mgtA →	magnesium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,911,210	0	C	T	48.0%	8.0 / 91.4	104	N734N (AAC→AAT)	mgtA	magnesium transporter
Reads supporting (aligned to +/- strand): ref base C (16/38); new base T (23/27); total (39/65)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 104 total aligned reads displayed.

CTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACG  >  minE/2911148‑2911262

cTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACctgctg                                                >  1:785235/1‑69 (MQ=255)

     aGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTAc                                             <  1:1034909/67‑1 (MQ=255)

     aGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTAc                                             <  1:2298884/67‑1 (MQ=255)

     aGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTAc                                             <  1:292117/67‑1 (MQ=255)

     aGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTAc                                             <  1:145394/67‑1 (MQ=255)

     aGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTAc                                             <  1:1958810/67‑1 (MQ=255)

     aGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTAc                                             <  1:1299877/67‑1 (MQ=255)

     aGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTAc                                             <  1:1793268/67‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATTTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:570129/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:2070080/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:566303/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:1777772/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:431016/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:388506/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:2684975/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:2110055/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:2877902/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:2407710/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:2418247/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:1044032/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:1287995/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:952186/69‑1 (MQ=255)

          gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:722483/69‑1 (MQ=255)

           tGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:231220/68‑1 (MQ=255)

           tGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATgtgt                                      <  1:2968864/68‑1 (MQ=255)

             cTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcac                                   >  1:2819312/1‑69 (MQ=255)

             cTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcac                                   >  1:277431/1‑69 (MQ=255)

             cTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcac                                   >  1:1855866/1‑69 (MQ=255)

             cTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcac                                   >  1:870716/1‑69 (MQ=255)

             cTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcac                                   >  1:1791736/1‑69 (MQ=255)

             cTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcac                                   >  1:707430/1‑69 (MQ=255)

             cTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcac                                   >  1:69370/1‑69 (MQ=255)

             cTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcac                                   >  1:1500311/1‑69 (MQ=255)

             cTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTca                                    >  1:2095117/1‑68 (MQ=255)

               ttCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcaca                                  <  1:1112619/68‑1 (MQ=255)

               ttCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcaca                                  <  1:1132515/68‑1 (MQ=255)

               ttCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcaca                                  <  1:763540/68‑1 (MQ=255)

               ttCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcaca                                  <  1:3195171/68‑1 (MQ=255)

               ttCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcaca                                  <  1:958111/68‑1 (MQ=255)

               ttCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcaca                                  <  1:2709133/68‑1 (MQ=255)

               ttCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcaca                                  <  1:2691474/68‑1 (MQ=255)

               ttCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcaca                                  <  1:5824/68‑1 (MQ=255)

               ttCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcaca                                  <  1:1348891/68‑1 (MQ=255)

               ttCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcaca                                  <  1:674512/68‑1 (MQ=255)

               ttCTTGACCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTcaca                                  <  1:511774/68‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:2775765/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:2438116/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:2824068/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:3016799/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:3117193/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:3121085/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:3216403/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:3257917/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:3326842/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:3429415/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:2026671/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:1773902/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:1570722/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:1521783/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:1794373/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:1817658/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:1819607/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:1397676/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:2162554/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:2201018/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:2548887/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:1097651/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:111332/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATctgctg                                                <  1:229608/49‑1 (MQ=255)

                    gCCCTTCCTGCCGATGTTGCC‑CTACACTTGCTTATTCAAAATctgctg                                                <  1:2921565/48‑1 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCCCAGGTGGCGa                          >  1:225912/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCg                           >  1:746513/1‑68 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:1015452/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:579286/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:1514203/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:833225/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:1484478/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:1339169/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:377009/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:1257940/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:1248333/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:403476/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:2299496/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:2824858/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:2050423/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:1878740/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:3251581/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:1790574/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:403426/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGAGTCACAGGTGGCGa                          >  1:1944069/1‑69 (MQ=255)

                      ccTTCCTGCCGATGTTGCCGCTACACTTGATTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGa                          >  1:2600831/1‑69 (MQ=255)

                      ccTTCCT‑CCGATGTTGCCGCTACACTTGCTTATTCAAAATCTGCTGTACGATGTGTCACAGGTGGCGAt                         >  1:1507199/1‑69 (MQ=255)

                           cTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTAc                                             <  1:1954877/45‑1 (MQ=255)

                                  tGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTAc                                             <  1:1012320/38‑1 (MQ=255)

                                            acacTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTcgacg     >  1:575510/1‑68 (MQ=255)

                                            acacTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTcgacg     >  1:3043943/1‑68 (MQ=255)

                                            acacTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTagacg     >  1:1784860/1‑68 (MQ=255)

                                               cTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTcgacgacg  >  1:338261/1‑68 (MQ=255)

                                               cTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTcgacgacg  >  1:689260/1‑68 (MQ=255)

                                               cTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTcgacgacg  >  1:2565060/1‑68 (MQ=255)

CTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACG  >  minE/2911148‑2911262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`