| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,237 | C→T | 47.0% | A153A (GCG→GCA) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,237 | 0 | C | T | 47.0% | 13.8 / 86.3 | 102 | A153A (GCG→GCA) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base C (38/16); new base T (20/28); total (58/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
GCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCA > minE/2919176‑2919303 | gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTg < 1:1072827/68‑1 (MQ=255)gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTg < 1:1939703/68‑1 (MQ=255)gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTg < 1:1879251/68‑1 (MQ=255)gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTg < 1:2081378/68‑1 (MQ=255)gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTg < 1:1644672/68‑1 (MQ=255)gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTg < 1:1644369/68‑1 (MQ=255)gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTg < 1:482966/68‑1 (MQ=255)gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTg < 1:525201/68‑1 (MQ=255)gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTg < 1:734643/68‑1 (MQ=255)gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTg < 1:874462/68‑1 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCggg > 1:1548677/1‑68 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCggg > 1:805939/1‑68 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCggg > 1:1155254/1‑68 (MQ=255) aTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa < 1:2069804/68‑1 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCaaa > 1:2386815/1‑69 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCaaa > 1:1090229/1‑69 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCaaa > 1:2104895/1‑69 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCaaa > 1:2297010/1‑69 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCaaa > 1:2299895/1‑69 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCaaa > 1:1647396/1‑69 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCaaa > 1:2719747/1‑69 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCaaa > 1:3251603/1‑69 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCaaa > 1:359157/1‑69 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCaa > 1:2282201/1‑68 (MQ=255) ccATGTTGTTACGCGCGTTACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGc < 1:1594738/68‑1 (MQ=255) ccATGTTGTTACGCGCGTTACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGc < 1:158994/68‑1 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc > 1:704842/1‑69 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc > 1:2533861/1‑69 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc > 1:2328560/1‑69 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc > 1:3184669/1‑69 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc > 1:3381440/1‑69 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc > 1:38789/1‑69 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc > 1:957977/1‑69 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc > 1:1538937/1‑69 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc > 1:1138392/1‑69 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc > 1:1736846/1‑69 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGCGCAAATGCTc > 1:2671091/1‑69 (MQ=255) ttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGc > 1:1889113/1‑69 (MQ=255) tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCa < 1:156312/57‑1 (MQ=255) tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCa < 1:1574607/57‑1 (MQ=255) tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCa < 1:2651936/57‑1 (MQ=255) tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCa < 1:2647841/57‑1 (MQ=255) tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCa < 1:2560099/57‑1 (MQ=255) tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCa < 1:1593198/57‑1 (MQ=255) tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCa < 1:1621420/57‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGTAAAt < 1:855115/52‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCAAAt < 1:2256612/52‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCAAAt < 1:1830965/52‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCAAAt < 1:2018641/52‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCAAAt < 1:3252431/52‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCAAAt < 1:3267956/52‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCAAAt < 1:3324886/52‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGCCGGGCAAAt < 1:223443/52‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGATGAATGCTTTGCCGGGCAAAt < 1:1587465/52‑1 (MQ=255) gTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGagaag < 1:1847192/69‑1 (MQ=255) gTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGagaag < 1:576188/69‑1 (MQ=255) gTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGagaag < 1:2032714/69‑1 (MQ=255) aCCTGCATAGACCAGCGTCATTTCGTTGAATGCTTTGACGGGCAAAt < 1:827500/47‑1 (MQ=255) cTGCATAGACCAGCGTCATTTCGTTGAATGCTTTg < 1:3078532/35‑1 (MQ=255) tGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATcc > 1:778813/1‑67 (MQ=255) tGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATcc > 1:151643/1‑67 (MQ=255) ccAGCGTCATTTCGTTGAACGCTTTGCCTGGCAAATGCTCCTGCAt > 1:16580/1‑46 (MQ=255) ccAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCAt > 1:1552741/1‑46 (MQ=255) ccAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCAt > 1:684310/1‑46 (MQ=255) ccAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCAt > 1:2705489/1‑46 (MQ=255) ccAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCAt > 1:1790155/1‑46 (MQ=255) ccAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCAt > 1:770269/1‑46 (MQ=255) cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg < 1:702588/69‑1 (MQ=255) cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg < 1:929533/69‑1 (MQ=255) cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg < 1:3318113/69‑1 (MQ=255) aGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg < 1:2970336/68‑1 (MQ=255) aGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg < 1:3025486/68‑1 (MQ=255) aGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg < 1:1159634/68‑1 (MQ=255) aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGa > 1:1397775/1‑69 (MQ=255) aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGa > 1:387736/1‑69 (MQ=255) aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGa > 1:1879786/1‑69 (MQ=255) aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGa > 1:2547192/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTgaggag > 1:1590967/1‑39 (MQ=37) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:2644717/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:1033824/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:2132764/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:1079628/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:21368/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:1743030/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:1686910/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:241786/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:773252/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:725419/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:2836573/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:671556/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:569326/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:2918987/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:3196566/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:3360658/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:1557457/1‑69 (MQ=255) gAATGCTTTGCCGGGCAAATGCTACTGCATGGTGAGGAGATCCGCCAGCAGCTGCGTGGGGTGGAACTc > 1:29096/1‑69 (MQ=255) aCGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa < 1:3390329/68‑1 (MQ=255) aCGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa < 1:2621723/68‑1 (MQ=255) aCGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa < 1:2914540/68‑1 (MQ=255) aCGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa < 1:3418362/68‑1 (MQ=255) | GCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCA > minE/2919176‑2919303 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |