| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,928,279 | C→T | 48.5% | D226D (GAC→GAT) | yjgP → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,928,279 | 0 | C | T | 48.5% | 7.8 / 126.8 | 140 | D226D (GAC→GAT) | yjgP | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (43/29); new base T (51/17); total (94/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.17e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
Only 100 of 140 total aligned reads displayed.
CGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCG > minE/2928213‑2928340 | cgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACt > 1:2293910/1‑68 (MQ=255)cgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACt > 1:1282367/1‑68 (MQ=255)cgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACt > 1:2448523/1‑68 (MQ=255) aCTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAg < 1:1747245/69‑1 (MQ=255) aCTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAg < 1:3384950/69‑1 (MQ=255) ctcAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGAt < 1:3145898/69‑1 (MQ=255) ctcAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGAt < 1:2844681/69‑1 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCa > 1:2376978/1‑60 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTAt > 1:1020749/1‑67 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATc > 1:1335076/1‑68 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATc > 1:770228/1‑68 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:2628036/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:2782598/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:296372/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:2158023/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:1897049/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:687140/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:1817771/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:3026762/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:1028983/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:75219/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:124667/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCa > 1:1305856/1‑69 (MQ=255) ccAGGGAACGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTAGAGGATTATCa > 1:2811875/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCa > 1:3387847/1‑63 (MQ=255) aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt > 1:2943134/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt > 1:1072498/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt > 1:3252975/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt > 1:1771553/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt > 1:752144/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGAt > 1:1430282/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCTAt > 1:2367003/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGa > 1:2433104/1‑68 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:523670/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:2717678/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:2648081/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:2729541/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:173895/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:150207/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:3294262/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:2750275/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:2457912/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:1388902/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:2409671/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:735904/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:1841482/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:594761/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:2201298/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:2199766/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:1162131/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:1688011/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:2061272/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:2045357/1‑69 (MQ=255) aaCGCGCTTCGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:1366422/1‑69 (MQ=255) aaCGCGCTTAGAAGGCACTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGAt > 1:2662412/1‑69 (MQ=255) aCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATc < 1:1234938/69‑1 (MQ=255) aCGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATc < 1:1082185/69‑1 (MQ=255) gcTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTg < 1:839/69‑1 (MQ=255) gcTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATAAGGCGATCATTg < 1:621075/69‑1 (MQ=255) ttCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGt < 1:627744/69‑1 (MQ=255) tCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATGATTGGt < 1:1202348/68‑1 (MQ=255) gCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAgg > 1:2644571/1‑69 (MQ=255) gCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAgg > 1:673194/1‑69 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg < 1:2604952/69‑1 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg < 1:3229095/69‑1 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg < 1:869248/69‑1 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg < 1:424736/69‑1 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg < 1:1171751/69‑1 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg < 1:2204523/69‑1 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg < 1:2100801/69‑1 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg < 1:2079061/69‑1 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg < 1:1871463/69‑1 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg < 1:2761111/69‑1 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCgg < 1:1345777/69‑1 (MQ=255) cTGCACTGTTACGTGATTTCCGCATTACGGATTTCCAGGAATATCAGGCGATCATTGGTCACCAGGCgg < 1:3330668/69‑1 (MQ=255) gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGt > 1:703303/1‑68 (MQ=255) gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg > 1:2608995/1‑69 (MQ=255) gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg > 1:1016396/1‑69 (MQ=255) gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg > 1:3317875/1‑69 (MQ=255) gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg > 1:2576425/1‑69 (MQ=255) gCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTg > 1:2487879/1‑69 (MQ=255) aCGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGa > 1:3096613/1‑69 (MQ=255) ttCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAAc < 1:69135/69‑1 (MQ=255) ttCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAAc < 1:3058320/69‑1 (MQ=255) tCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAAc < 1:1594638/68‑1 (MQ=255) cATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATa < 1:2382283/68‑1 (MQ=255) cATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATa < 1:2980005/68‑1 (MQ=255) cATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATa < 1:901703/68‑1 (MQ=255) cATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATa < 1:936167/68‑1 (MQ=255) cATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATa < 1:973465/68‑1 (MQ=255) aTTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATa < 1:3158479/67‑1 (MQ=255) aTTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATAcc < 1:2278406/69‑1 (MQ=255) aTTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATAcc < 1:3324430/69‑1 (MQ=255) aTTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATAcc < 1:1822260/69‑1 (MQ=255) aCGGACTTCCAGGATTATCAGGCGATCATTGGTCACCa > 1:1721201/1‑38 (MQ=255) aCGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCg > 1:665289/1‑67 (MQ=255) aCGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCg > 1:2408184/1‑67 (MQ=255) aCGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCg > 1:748457/1‑67 (MQ=255) aCGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCg > 1:1538358/1‑67 (MQ=255) aCGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCg > 1:2989125/1‑67 (MQ=255) | CGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCG > minE/2928213‑2928340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |