| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 253,007 | C→T | 50.6% | L127L (TTG→TTA) | yajB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 253,007 | 0 | C | T | 50.6% | ‑0.1 / 71.0 | 77 | L127L (TTG→TTA) | yajB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (22/16); new base T (24/15); total (46/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGG > minE/252941‑253074 | cATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACcac < 1:1871907/67‑1 (MQ=255) tGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGt < 1:3337756/69‑1 (MQ=255) ccGTTTAACACGTTCTGGATGAAATCCATATCTCGATAGCGCACCAGCCACTGCTCTGACCACAAGTaa > 1:1133767/1‑69 (MQ=255) ccGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTaa > 1:2021513/1‑69 (MQ=255) ccGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTaa > 1:1415454/1‑69 (MQ=255) ccGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTaa > 1:1613007/1‑69 (MQ=255) ccGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTaa > 1:157739/1‑69 (MQ=255) ccGTATAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTa > 1:1344380/1‑68 (MQ=255) cacGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa < 1:1818970/68‑1 (MQ=255) cacGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa < 1:2672577/68‑1 (MQ=255) cacGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa < 1:2654531/68‑1 (MQ=255) cacGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa < 1:2817579/68‑1 (MQ=255) cacGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa < 1:2968124/68‑1 (MQ=255) cacGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa < 1:3197189/68‑1 (MQ=255) cacGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa < 1:379933/68‑1 (MQ=255) cacGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa < 1:1437563/68‑1 (MQ=255) cacGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa < 1:1195761/68‑1 (MQ=255) cacGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa < 1:615763/68‑1 (MQ=255) cTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGa > 1:1597218/1‑68 (MQ=255) cTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGa > 1:1664991/1‑68 (MQ=255) cTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGa > 1:2818090/1‑68 (MQ=255) cgATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATaa > 1:2751746/2‑69 (MQ=255) gAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATaaaatg > 1:2472950/1‑67 (MQ=255) gAAATCCATATCGCGATAGCGCACAAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACg > 1:1799779/1‑69 (MQ=255) aaaTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa > 1:539004/1‑55 (MQ=255) aaaTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa > 1:2297069/1‑55 (MQ=255) aaaTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa > 1:1935865/1‑55 (MQ=255) aaaTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa > 1:2950221/1‑55 (MQ=255) aaaTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa > 1:2498018/1‑55 (MQ=255) aaaTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa > 1:1874934/1‑55 (MQ=255) aaaTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa > 1:305653/1‑55 (MQ=255) aaaTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa > 1:1100485/1‑55 (MQ=255) aaaTCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCa > 1:1595548/1‑55 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:1002401/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2768636/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2589113/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2853650/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2166070/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2855718/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:1254750/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2003731/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2917401/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2975837/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:1809219/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:3194728/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:490115/69‑2 (MQ=255) ccAACCACTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2429672/68‑2 (MQ=255) ccAACCACTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:1763570/68‑2 (MQ=255) ccACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGtcat < 1:1264403/69‑1 (MQ=255) ccACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGtcat < 1:1528940/69‑1 (MQ=255) gCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACtt > 1:1833913/1‑69 (MQ=255) ctctGACCACAAGTAATTGTTCAGATTGATAAAACGTGGc < 1:2318949/40‑1 (MQ=255) ctctGACCACAAGTAATTGTTCAGATTGATAAAACGTGGc < 1:2079556/40‑1 (MQ=255) ctctGACCACAAGTAATTGTTCAGATTGATAAAACGTGGc < 1:1432341/40‑1 (MQ=255) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCg > 1:2431560/1‑39 (MQ=18) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:88859/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:666062/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1258107/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:545916/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1615491/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:508192/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:136049/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:423/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:395572/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:924416/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:3295866/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:2727952/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:3189399/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:3180355/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:3172654/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1901592/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1908354/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1916792/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1931129/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:2203408/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1000458/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:2588595/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatc > 1:920606/1‑62 (MQ=19) acaAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCggg < 1:3397077/69‑1 (MQ=255) | CATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGG > minE/252941‑253074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |