| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,949,029 | T→C | 25.8% | A40A (GCA→GCG) | yjjP ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,949,029 | 0 | T | C | 25.8% | 88.9 / 30.4 | 81 | A40A (GCA→GCG) | yjjP | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (19/41); new base C (19/2); total (38/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.53e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01 | |||||||||||
TTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGAAATAA > minE/2948966‑2949097 | tttAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCg > 1:2441216/1‑69 (MQ=255)tttAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCg > 1:1474122/1‑69 (MQ=255)tttAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCg > 1:1807594/1‑69 (MQ=255) aTAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGAc < 1:3207823/67‑1 (MQ=255) aTAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGAc < 1:2705771/67‑1 (MQ=255) aTAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGAc < 1:435751/67‑1 (MQ=255) gTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTc > 1:2406012/1‑67 (MQ=255) aGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAg < 1:1019658/69‑1 (MQ=255) aGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAg < 1:3073478/69‑1 (MQ=255) aGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAg < 1:2677414/69‑1 (MQ=255) aGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAg < 1:2411492/69‑1 (MQ=255) gCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAg < 1:636654/68‑1 (MQ=255) aCTATGGCGTTCGAATAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGaa < 1:1533563/69‑1 (MQ=255) aCTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGaa < 1:892442/69‑1 (MQ=255) aCTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGaa < 1:356668/69‑1 (MQ=255) aCTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGaa < 1:1346807/69‑1 (MQ=255) aCTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGaa < 1:1528515/69‑1 (MQ=255) aCTATGGCGTGCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGaa < 1:3257170/69‑1 (MQ=255) aTGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAg > 1:1547165/1‑68 (MQ=255) ttCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGCCCCAGTCGTg > 1:2607127/1‑55 (MQ=255) ttCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAgg > 1:135601/1‑58 (MQ=255) ttCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGaa > 1:1113495/1‑60 (MQ=255) ttCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCtcatca > 1:3044870/1‑69 (MQ=255) ttCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCtcatca > 1:3082763/1‑69 (MQ=255) ttCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCtcatca > 1:398744/1‑69 (MQ=255) ttCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCtcat > 1:2546524/1‑67 (MQ=255) ttCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCtaatca > 1:2696841/1‑69 (MQ=255) gaggTAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCt < 1:1573179/58‑1 (MQ=255) gagaTAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCt < 1:597368/58‑1 (MQ=255) gagaTAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCt < 1:75755/58‑1 (MQ=255) gagaTAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCt < 1:1528759/58‑1 (MQ=255) gagaTAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCt < 1:896593/58‑1 (MQ=255) gagaTAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCt < 1:162228/58‑1 (MQ=255) gagaTAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACACAGTCGTGAGGAAAGCt < 1:1571492/58‑1 (MQ=255) aGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACgcgc > 1:2361965/1‑68 (MQ=255) aGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACgcgc > 1:1921621/1‑68 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:2833823/69‑1 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:3027435/69‑1 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:35430/69‑1 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:2687286/69‑1 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:264278/69‑1 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:392147/69‑1 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:41752/69‑1 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:1471305/69‑1 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:2364348/69‑1 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:1825114/69‑1 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:1701014/69‑1 (MQ=255) aCTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTc < 1:713739/69‑1 (MQ=255) gACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCa < 1:1016262/67‑1 (MQ=255) gACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCa < 1:99487/67‑1 (MQ=255) gACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCa < 1:2788916/67‑1 (MQ=255) cGCTGTCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCAt < 1:2532768/69‑1 (MQ=255) gCTGTCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCAt < 1:3284522/68‑1 (MQ=255) tCCATTCCCAACGCCCGCCCCAGTCGTGAGGAAAGCtcatc > 1:2756318/1‑41 (MQ=37) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATcaaccaa > 1:713827/1‑47 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCAt > 1:2318045/1‑64 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:2898252/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:99128/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:1514105/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:1608525/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:169335/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:600206/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:526813/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:2433384/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:2533083/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:3406589/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:337170/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:2768694/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:2929252/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:3167471/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:314488/1‑68 (MQ=255) tCCATTCCCAACGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTg > 1:2849486/1‑68 (MQ=255) cccAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGa > 1:91220/1‑69 (MQ=255) cccAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGa > 1:3176089/1‑69 (MQ=255) cAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCttt > 1:87721/1‑47 (MQ=255) cAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGaa > 1:1134434/1‑68 (MQ=255) cAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGaa < 1:1288917/68‑1 (MQ=255) cAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGaa < 1:2896322/68‑1 (MQ=255) cAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGaa < 1:1663295/68‑1 (MQ=255) aGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGAaat < 1:472303/69‑1 (MQ=255) aGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGAaat < 1:2735486/69‑1 (MQ=255) tGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGAaataa > 1:2204290/1‑69 (MQ=255) tGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGAaataa > 1:3218172/1‑69 (MQ=255) | TTTAATAGTTGTCAGCACTATGGCGTTCGAAGAGATAGAACTTTCGACGCTGTCCATTCCCAGTGCCCGACCCAGTCGTGAGGAAAGCTCATCAACCAACGCGCTTTCCGCACCATGTTGCAAAAGAAATAA > minE/2948966‑2949097 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |