| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,950,906 | T→C | 63.3% | L135L (TTA→CTA) | bglJ → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,950,906 | 0 | T | C | 63.3% | 21.1 / 19.4 | 38 | L135L (TTA→CTA) | bglJ | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (6/8); new base C (8/16); total (14/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAAT > minE/2950842‑2950958 | aaaTGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAg > 1:1735234/1‑69 (MQ=255) tGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGccc > 1:346775/1‑69 (MQ=255) gtACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGa > 1:953660/1‑67 (MQ=255) gtACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGa > 1:637548/1‑67 (MQ=255) gtACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGa > 1:3430611/1‑67 (MQ=255) gtACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGa > 1:1797554/1‑67 (MQ=255) gtACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGa > 1:1883098/1‑67 (MQ=255) gtACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGa > 1:2814926/1‑67 (MQ=255) gtACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGa > 1:2474903/1‑67 (MQ=255) gtACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACAAAAGCCGGACGCTAAGCCCGa > 1:2737551/1‑67 (MQ=255) cGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGga < 1:65946/68‑1 (MQ=255) cAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGACGgaggga < 1:3198932/69‑1 (MQ=255) cAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGACGgagaga < 1:1345029/69‑1 (MQ=255) cAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGACGgagaga < 1:2926989/69‑1 (MQ=255) cAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGACGgagaga < 1:1410383/69‑1 (MQ=255) cAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACAAAAGCCGGACGCTAAGCCCGACGgagaga < 1:1704595/69‑1 (MQ=255) aGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGgagaga > 1:1946829/1‑68 (MQ=255) aGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGgagaga > 1:2518630/1‑68 (MQ=255) aGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGCTAAGCCCGACGgtgaga < 1:2015459/68‑1 (MQ=255) aCATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGaaa < 1:693197/42‑1 (MQ=255) aCATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGaaa < 1:1788326/42‑1 (MQ=255) aCATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGaaa < 1:1762095/42‑1 (MQ=255) cATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTc > 1:3235170/1‑69 (MQ=255) ttAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCTCGTGGc < 1:1542948/62‑1 (MQ=255) tAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAAt < 1:2588130/69‑1 (MQ=255) tAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAAt < 1:1680683/69‑1 (MQ=255) aaCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAAt < 1:2383424/68‑1 (MQ=255) aaCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAAt > 1:2732632/1‑68 (MQ=255) cAAAGCCGGACGCTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGt < 1:1076246/54‑1 (MQ=255) cAAAGCCGGACGCTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGt < 1:445273/54‑1 (MQ=255) cAAAGCCGGACGCTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGt < 1:3376645/54‑1 (MQ=255) cAAAGCCGGACGCTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGt < 1:190879/54‑1 (MQ=255) cAAAGCCGGACGCTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGt < 1:1921280/54‑1 (MQ=255) cAAAGCCGGACGCTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGt < 1:2622999/54‑1 (MQ=255) cAAAGCCGGACGCTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGt < 1:2592548/54‑1 (MQ=255) cAAAGCCGGACGCTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGt < 1:2455381/54‑1 (MQ=255) cAAAGCCGGACGCTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGt < 1:2121187/54‑1 (MQ=255) cAAAGCCCGACGCTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGt < 1:78447/54‑1 (MQ=255) | AAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAAT > minE/2950842‑2950958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |