| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,951,444 | T→C | 24.7% | Q170Q (CAA→CAG) | fhuF ← | ferric iron reductase involved in ferric hydroximate transport |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,951,444 | 0 | T | C | 24.7% | 138.5 / 41.0 | 113 | Q170Q (CAA→CAG) | fhuF | ferric iron reductase involved in ferric hydroximate transport |
| Reads supporting (aligned to +/- strand): ref base T (51/34); new base C (25/3); total (76/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.24e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTG > minE/2951381‑2951505 | gATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTTTAGTGCTTGcac > 1:672389/1‑69 (MQ=255)gATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGcac > 1:2133545/1‑69 (MQ=255)gATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGcac > 1:1747178/1‑69 (MQ=255)gATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGcac > 1:194906/1‑69 (MQ=255)gATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGcac > 1:690389/1‑69 (MQ=255)gATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGcac > 1:3155695/1‑69 (MQ=255)gATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGcac > 1:479191/1‑69 (MQ=255)gATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGcac > 1:854750/1‑69 (MQ=255)gATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGcac > 1:909085/1‑69 (MQ=255)gATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGcac > 1:1199276/1‑69 (MQ=255)gATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGcac > 1:230608/1‑69 (MQ=255) cAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAAc > 1:1538575/1‑69 (MQ=255) cAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAAc < 1:2481635/69‑1 (MQ=255) cAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAAc < 1:3124557/69‑1 (MQ=255) aTTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGa > 1:667189/1‑61 (MQ=255) aTTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAgcgc > 1:1195730/1‑69 (MQ=255) aTTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAgcgc > 1:2044942/1‑69 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCGCAACCGGAACCAGCGCCTg > 1:813632/1‑69 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCgg > 1:528748/1‑57 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGa > 1:1577717/1‑58 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAAcc < 1:1753213/61‑1 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAAcc < 1:2882900/61‑1 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGcc > 1:1351390/1‑67 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCt > 1:1568557/1‑68 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTg > 1:585901/1‑69 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTg > 1:2553467/1‑69 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTg > 1:2686237/1‑69 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTg > 1:1928466/1‑69 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTg > 1:796267/1‑69 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTg > 1:918892/1‑69 (MQ=255) aCTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCGTCTAGTGCTTGCACAACCGGAACCa > 1:633862/1‑62 (MQ=255) cTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGcaca > 1:1572349/1‑51 (MQ=255) cTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCg > 1:3043877/1‑55 (MQ=255) cTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAAcc > 1:366181/1‑60 (MQ=255) cTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAAcc > 1:2133789/1‑60 (MQ=255) cTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAAcc > 1:3004114/1‑60 (MQ=255) cTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAAcc > 1:1380353/1‑60 (MQ=255) cTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAAcc > 1:1740141/1‑60 (MQ=255) tAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTa > 1:449411/1‑69 (MQ=255) aaGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTaa < 1:2184935/69‑1 (MQ=255) aaGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTaa > 1:1872049/1‑69 (MQ=255) aaGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTaa > 1:2462026/1‑69 (MQ=255) aaGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTaa > 1:441505/1‑69 (MQ=255) aaGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTaa < 1:598413/69‑1 (MQ=255) aaGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTaa > 1:1517488/1‑69 (MQ=255) aaGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTa > 1:258505/1‑68 (MQ=255) aGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTaa < 1:2579235/68‑1 (MQ=255) aGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAAc < 1:3850/69‑1 (MQ=255) aGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAAc < 1:687053/69‑1 (MQ=255) gTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAAc < 1:3286193/43‑1 (MQ=255) tttGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGt < 1:2229139/69‑1 (MQ=255) tttGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGt < 1:485128/69‑1 (MQ=255) gagctctcCGGTCGCTTCTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGtt > 1:1464785/4‑61 (MQ=255) gATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCg > 1:944731/1‑69 (MQ=255) gATCTCTCCGGTCGCTTCTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCAtt > 1:118019/1‑67 (MQ=255) gATCTCTCCGGTCGCTTCTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCg > 1:1619914/1‑69 (MQ=255) gATCTCTCCGGTCGCTTCTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCg > 1:2687350/1‑69 (MQ=255) gATCTCTCCGGTCGCTTCTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCg > 1:3366761/1‑69 (MQ=255) gATCTCTCCGGTCGCTTCTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCg > 1:3003378/1‑69 (MQ=255) cGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGa > 1:1158427/1‑69 (MQ=255) cGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGa > 1:1572354/1‑69 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACGAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:670115/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:1138418/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:2033586/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:503903/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:351684/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:601609/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:1666838/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:3144271/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:1341708/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:801556/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:3075890/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:2689273/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:131704/69‑1 (MQ=255) gCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGaa < 1:1279786/69‑1 (MQ=255) cTTCTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAAc < 1:1396382/44‑1 (MQ=255) ttCTAGTGCTT‑CACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAAtgt < 1:1715789/69‑1 (MQ=255) tCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAAtgt < 1:1743350/69‑1 (MQ=255) tCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAAtgt < 1:2838831/69‑1 (MQ=255) tCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAAtgt < 1:2109732/69‑1 (MQ=255) tCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAAtgt < 1:2724361/69‑1 (MQ=255) cTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAAtgtg > 1:1190807/1‑69 (MQ=255) cTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAAtgtg > 1:2735503/1‑69 (MQ=255) cTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAAtgtg > 1:3100235/1‑69 (MQ=255) cTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAAtgtg > 1:2041667/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACTTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:1362539/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGa > 1:3407375/1‑64 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:2400276/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:2851267/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:128895/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:2502376/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:2451538/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:1487136/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:629302/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:3429277/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:1686338/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:2148879/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:2117357/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtgtg > 1:2000681/1‑69 (MQ=255) cTAGTGCCTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGCTGCTGCGGCGAAtg > 1:2239587/1‑67 (MQ=255) | GATCAAATAACCGGTATTACTCCAGATAAGTTTGCCGTTGATCTCTCCGGTCGCTTCTAGTGCTTGCACAACCGGAACCAGCGCCTGGCTGATTAACGTTTCCATTCGATGCTGCGGCGAATGTG > minE/2951381‑2951505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |