Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,954,513 | G→A | 63.5% | P13P (CCG→CCA) | rimI → | acetylase for 30S ribosomal subunit protein S18 |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,954,513 | 0 | G | A | 63.5% | 34.1 / 37.9 | 63 | P13P (CCG→CCA) | rimI | acetylase for 30S ribosomal subunit protein S18 |
Reads supporting (aligned to +/- strand): ref base G (16/7); new base A (17/23); total (33/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.57e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 |
CGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACG > minE/2954451‑2954567 | cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAgctgct < 1:3297299/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:971409/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:2365717/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:2406522/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:2078817/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:2506051/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:1750366/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:257447/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:1657533/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:2677809/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:1649783/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:2705997/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:1559800/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:3115654/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:1528990/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:418529/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:1298758/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:515671/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:1250289/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:555147/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:598658/69‑1 (MQ=255) cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCt < 1:1157511/69‑1 (MQ=255) tGGCAACAAATTTTCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGTCt < 1:332150/64‑1 (MQ=255) ggCAACAAATTTGCACATATGAACACGATTTCTTCCCTCTAAACGACTGATTTACCGGCGGCTTACca < 1:2308998/68‑1 (MQ=255) ggCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACca < 1:1613751/68‑1 (MQ=255) ggCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACca < 1:481949/68‑1 (MQ=255) aaaTTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGa > 1:1070444/1‑68 (MQ=255) aaaTTTGCACATATGAACACGATTTCTTCCCTCGAAACGAATGATTTACCGGCGGCTTACCACATTGa > 1:1467647/1‑68 (MQ=255) caTATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc < 1:2366870/69‑1 (MQ=255) caTATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc < 1:2372810/69‑1 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGGGcc > 1:3063500/1‑67 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc > 1:2290443/1‑67 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc > 1:1002957/1‑67 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc > 1:537672/1‑67 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc > 1:1278550/1‑67 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc > 1:490430/1‑67 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc > 1:432022/1‑67 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc > 1:1336430/1‑67 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc > 1:2201821/1‑67 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc > 1:2180656/1‑67 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc > 1:2482225/1‑67 (MQ=255) tatGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGcc > 1:2172391/1‑67 (MQ=255) atGAATACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:487371/1‑69 (MQ=255) atGAACCCGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGcc > 1:673597/1‑66 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:1654222/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:76451/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:701419/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:624668/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:1213617/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:241438/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:398746/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:1482652/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:3279365/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:155596/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:1932683/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:2838685/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:1598173/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCAGCGGCTTACCACATTGAACAACGCGCCCAc > 1:1687329/1‑69 (MQ=255) atGAACACGATTTCTTCCCTCGAAACGACTGAATTACCAGCGGCATACCCCATAGAACAAcg > 1:2894708/1‑62 (MQ=255) tGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTAGCACATTGAACAACGCGCCCACg > 1:2404198/1‑69 (MQ=255) tGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACg > 1:224556/1‑69 (MQ=255) aCGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGTAGTGAAAAAACg < 1:1232725/69‑1 (MQ=255) aCGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACg < 1:1310226/69‑1 (MQ=255) | CGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACG > minE/2954451‑2954567 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |